Protein Information

Cytoscape View


UniProt AC / UniProt ID Q13464 / ROCK1_HUMAN
Protein Name Rho-associated protein kinase 1
Gene Name Name: ROCK1
Organism Homo sapiens (Human)

PRO ID PR:Q13464
DAG View
PRO Name Rho-associated protein kinase 1 (human)
Definition A Rho-associated protein kinase 1 that is encoded in the genome of human.
Short Label hROCK1
Category organism-gene

Interactive Sequence View

Select/align proteoforms across species


Q13464 (ROCK1) as Substrate



Site PTM Type PTM Enzyme Score Source PMID
S2 Acetylation UniProt 22814378
S2 Phosphorylation PhosphoSitePlus
T3 Phosphorylation PhosphoSitePlus
K13 Ubiquitination PhosphoSitePlus
K65 Ubiquitination PhosphoSitePlus
K80 Ubiquitination PhosphoSitePlus
K109 Ubiquitination PhosphoSitePlus
S118 Phosphorylation PhosphoSitePlus
K200 Ubiquitination PhosphoSitePlus
K225 Ubiquitination PhosphoSitePlus
Y255 Phosphorylation PhosphoSitePlus
S282 Phosphorylation PhosphoSitePlus
K294 Ubiquitination PhosphoSitePlus
K307 Ubiquitination PhosphoSitePlus
K339 Ubiquitination PhosphoSitePlus
T398 Phosphorylation PhosphoSitePlus
Y405 Phosphorylation PhosphoSitePlus
S407 Phosphorylation PhosphoSitePlus
K430 Ubiquitination PhosphoSitePlus
T455 Phosphorylation HPRD   PhosphoSitePlus 18767875
S456 Phosphorylation HPRD   PhosphoSitePlus 18767875
S533 Phosphorylation PhosphoSitePlus
K539 Ubiquitination PhosphoSitePlus
S567 Phosphorylation PhosphoSitePlus
T569 Phosphorylation PhosphoSitePlus
S572 Phosphorylation PhosphoSitePlus
S574 Phosphorylation PhosphoSitePlus
S576 Phosphorylation PhosphoSitePlus
S580 Phosphorylation PhosphoSitePlus
Y603 Phosphorylation PhosphoSitePlus
Y604 Phosphorylation PhosphoSitePlus
K647 Acetylation PhosphoSitePlus   UniProt 19608861
S679 Phosphorylation PhosphoSitePlus
S710 Phosphorylation PhosphoSitePlus
K718 Acetylation PhosphoSitePlus
S742 Phosphorylation PhosphoSitePlus
K782 Ubiquitination PhosphoSitePlus
K857 Ubiquitination PhosphoSitePlus
T887 Phosphorylation PhosphoSitePlus
T890 Phosphorylation PhosphoSitePlus
T897 Phosphorylation PhosphoSitePlus
K898 Ubiquitination PhosphoSitePlus
Y913 Phosphorylation PhosphoSitePlus
T917 Phosphorylation PhosphoSitePlus
K921 Ubiquitination PhosphoSitePlus
K950 Ubiquitination PhosphoSitePlus
T1024 Phosphorylation P41743 (PRKCI) neXtProt   PhosphoSitePlus 21685893
K1057 Ubiquitination PhosphoSitePlus
K1093 Ubiquitination PhosphoSitePlus
S1098 Phosphorylation HPRD   phospho.ELM   PhosphoSitePlus 18669648
S1100 Phosphorylation HPRD   phospho.ELM   PhosphoSitePlus 18669648
T1101 Phosphorylation HPRD   phospho.ELM   PhosphoSitePlus 18669648, 20068231
S1102 Phosphorylation HPRD   phospho.ELM   PhosphoSitePlus 19651622, 18669648
S1105 Phosphorylation HPRD   PhosphoSitePlus   UniProt 18691976, 20068231, 19690332, ...19651622
S1108 Phosphorylation PhosphoSitePlus
T1112 Phosphorylation PhosphoSitePlus
S1119 Phosphorylation PhosphoSitePlus
Y1153 Phosphorylation PhosphoSitePlus
T1180 Phosphorylation HPRD   phospho.ELM   PhosphoSitePlus 17525332
T1189 Phosphorylation PhosphoSitePlus
K1194 Acetylation PhosphoSitePlus
Y1287 Phosphorylation PhosphoSitePlus
S1322 Phosphorylation PhosphoSitePlus
S1328 Phosphorylation PhosphoSitePlus   UniProt 23186163
T1331 Phosphorylation PhosphoSitePlus
S1333 Phosphorylation P41743 (PRKCI) neXtProt   PhosphoSitePlus   RLIMS-P 24168723, 21685893
T1334 Phosphorylation P41743 (PRKCI) neXtProt   PhosphoSitePlus 21685893
S1336 O-Glycosylation GlyGen 28510447, 29351928
S1336 Phosphorylation PhosphoSitePlus
T1337 Phosphorylation P41743 (PRKCI) neXtProt   PhosphoSitePlus 21685893
S1341 Phosphorylation P41743 (PRKCI) neXtProt   PhosphoSitePlus 21685893


Q13464 (ROCK1) as PTM Enzyme



Substrate Site Score Source PMID
O00429 (DNM1L) S637 neXtProt 22326220
O14649 (KCNK3) S336 Signor 21838752
O14649 (KCNK3) S393 Signor 21838752
O14950 (MYL12B) T19 PhosphoSitePlus   Signor 15226371, 12185584
O14950 (MYL12B) S20 PhosphoSitePlus   Signor 12185584, 15676024, 15226371, ...18648664, 10233893
O14974-1 (PPP1R12A) T696 HPRD   phospho.ELM   PhosphoSitePlus   Signor 8662509, 18212344, 11719507, ...18767875, 11931630, 15281089, 12600888, 12220642, 18669648, 10601309, 11384979, 18077418
O14974 (PPP1R12A) S852 PhosphoSitePlus 15676024, 10926869
O14974-1 (PPP1R12A) T853 HPRD   PhosphoSitePlus 10601309, 12220642, 19255442, ...12563012
O43293 (DAPK3) T265 neXtProt   PhosphoSitePlus 17158456
O43293 (DAPK3) T299 neXtProt   PhosphoSitePlus 17158456, 20854903
O60237 (PPP1R12B) T646 PhosphoSitePlus   Signor 22937917, 16431080
P04637 (TP53) S15 RLIMS-P+ 28131915
P07737 (PFN1) S138 neXtProt   PhosphoSitePlus   Signor 22479341, 18573880
P08670 (VIM) S72 HPRD   phospho.ELM   PhosphoSitePlus 15676024, 19007248, 18669648, ...9565595, 20166139, 23737341, 20068231
P14136-1 (GFAP) T7 HPRD   phospho.ELM   PhosphoSitePlus 12686604, 9099667
P14136-1 (GFAP) S13 HPRD   phospho.ELM   PhosphoSitePlus 7822264, 12686604, 9099667, ...2155236, 2114109
P14136-1 (GFAP) S17 HPRD 7822264, 2155236, 2114109
P14136-1 (GFAP) S38 HPRD   phospho.ELM   PhosphoSitePlus 7822264, 12686604, 9099667, ...2155236, 2114109
P15311 (EZR) T567 HPRD   phospho.ELM   PhosphoSitePlus   Signor 10970850, 19255442, 21979959, ...11387207, 19386264
P17661 (DES) S12 PhosphoSitePlus 12686604
P17661 (DES) T17 HPRD   PhosphoSitePlus   Signor 12686604, 10574968
P17661 (DES) S60 PhosphoSitePlus 12686604
P17661 (DES) T76 HPRD   PhosphoSitePlus   Signor 12686604, 10574968
P17661 (DES) T77 HPRD   PhosphoSitePlus   Signor 12686604, 10574968
P19105 (MYL12A) T18 phospho.ELM   Signor 12185584
P19105 (MYL12A) S19 phospho.ELM   Signor 12185584
P19634 (SLC9A1) T653 PhosphoSitePlus 25578862
P24844 (MYL9) T19 PhosphoSitePlus 26169356
P24844 (MYL9) S20 PhosphoSitePlus   Signor 26169356, 12802051, 8702756
P26038 (MSN) T558 HPRD   PhosphoSitePlus 19255442, 9516463, 9856983
P29966 (MARCKS) S159 PhosphoSitePlus 11162562
P35241-1 (RDX) T564 HPRD   PhosphoSitePlus 9456324, 19255442, 15676024, ...20068231
P35241-1 (RDX) T573 HPRD 9456324
P35611 (ADD1) T445 HPRD   phospho.ELM   PhosphoSitePlus   Signor 10209029
P35611-1 (ADD1) T480 HPRD   PhosphoSitePlus   Signor 10209029
P35611-3 (ADD1) T511 HPRD 10209029
P48436 (SOX9) S181 phospho.ELM   PhosphoSitePlus   Signor 20039424
P49815 (TSC2) T1203 RLIMS-P+ 21561859
P53667-1 (LIMK1) T508 HPRD   neXtProt   PhosphoSitePlus   Signor 11340065, 10559936, 10652353
P53671 (LIMK2) T505 HPRD   neXtProt   PhosphoSitePlus   Signor 11340065, 11171090, 11018042
P53671-1 (LIMK2) T526 HPRD 11340065, 11018042
P60484 (PTEN) S229 phospho.ELM   PhosphoSitePlus   Signor 15793569
P60484 (PTEN) T232 phospho.ELM   PhosphoSitePlus   Signor 15793569
P61587 (RND3) S7 PhosphoSitePlus 15775972
P61587 (RND3) S11 PhosphoSitePlus   Signor 15775972
P61587 (RND3) S218 PhosphoSitePlus 23622247
P61587 (RND3) S240 PhosphoSitePlus 23622247
Q05397 (PTK2) S732 neXtProt   phospho.ELM   PhosphoSitePlus 16760434
Q14160 (SCRIB) S1378 PhosphoSitePlus 26101221
Q14160 (SCRIB) S1508 PhosphoSitePlus 26101221
Q16555 (DPYSL2) T555 HPRD   PhosphoSitePlus 22443207, 10818093
Q8N264-1 (ARHGAP24) S391 HPRD   neXtProt   PhosphoSitePlus   Signor 16862148
Q8N264-1 (ARHGAP24) S402 HPRD   neXtProt   PhosphoSitePlus   Signor 16862148
Q8N264 (ARHGAP24) S413 HPRD   neXtProt   PhosphoSitePlus   Signor 16862148
Q8N264 (ARHGAP24) S415 HPRD   neXtProt   PhosphoSitePlus   Signor 16862148
Q8N264 (ARHGAP24) S437 HPRD   neXtProt   PhosphoSitePlus   Signor 16862148
Q8N264 (ARHGAP24) T452 HPRD   neXtProt   PhosphoSitePlus   Signor 16862148
Q8N264 (ARHGAP24) S573 HPRD   Signor 16862148
Q8N264 (ARHGAP24) S574 HPRD   Signor 16862148
Q8N264 (ARHGAP24) T575 HPRD   Signor 16862148
Q8N264-1 (ARHGAP24) T576 HPRD   Signor 16862148
Q8N264 (ARHGAP24) T577 HPRD   Signor 16862148
Q9NRY4 (ARHGAP35) S1150 PhosphoSitePlus   Signor 19103606
Q9NRY4 (ARHGAP35) T1173 PhosphoSitePlus 19103606
Q9NRY4 (ARHGAP35) S1174 PhosphoSitePlus 19103606
Q9NRY4 (ARHGAP35) T1226 PhosphoSitePlus 19103606
Q9NRY4 (ARHGAP35) S1236 PhosphoSitePlus   Signor 19103606
Q9UPT6 (MAPK8IP3) S314 PhosphoSitePlus   Signor 15767678, 19036714
Q9UPT6 (MAPK8IP3) S364 PhosphoSitePlus   Signor 15767678, 19036714
Q9UPT6 (MAPK8IP3) S365 PhosphoSitePlus   Signor 15767678, 19036714
Q9Y613 (FHOD1) S1131 PhosphoSitePlus   Signor 18239683
Q9Y613 (FHOD1) S1137 PhosphoSitePlus   Signor 18239683
Q9Y613 (FHOD1) T1141 PhosphoSitePlus   Signor 18239683


PTM-dependent PPI

PTM type Substrate Site Interactant Association type Source PMID
Phosphorylation P12931 (SRC) S43 Q13464 (ROCK1) unknown eFIP 33388549


PTM sites affected in variants

Site Variant Source PMID Disease [Sample source]
K65 E65 Biomuta DOID:363 / uterine cancer [ cosmic, icgc, tcga ]
K65 N65 Biomuta DOID:9256 / colorectal cancer [ icgc, tcga ] DOID:1909 / melanoma [ cosmic ]
K65 I65 Biomuta DOID:363 / uterine cancer [ cosmic, icgc, tcga ]
K109 E109 Biomuta DOID:11934 / head and neck cancer [ tcga ]
K109 I109 Biomuta DOID:9256 / colorectal cancer [ icgc, tcga ] DOID:1909 / melanoma [ cosmic ]
S118 F118 Biomuta DOID:11934 / head and neck cancer [ tcga ]
K225 R225 Biomuta DOID:363 / uterine cancer [ tcga ]
K225 * Biomuta DOID:3571 / liver cancer [ icgc ] DOID:0070003 / blastoma [ cosmic ]
T398 I398 Biomuta DOID:3963 / thyroid carcinoma [ cosmic, icgc ]
S407 C407 Biomuta DOID:10283 / prostate cancer [ cosmic ]
T455 N455 Biomuta DOID:11054 / urinary bladder cancer [ tcga ]
T455 I455 Biomuta DOID:1909 / melanoma [ tcga ]
S533 * Biomuta DOID:1612 / breast cancer [ cosmic ]
S572 N572 Biomuta DOID:363 / uterine cancer [ tcga ]
S574 L574 Biomuta DOID:11934 / head and neck cancer [ tcga ]
Y604 * Biomuta DOID:3571 / liver cancer [ icgc ]
S679 * Biomuta DOID:10534 / stomach cancer [ tcga ]
K718 T718 Biomuta DOID:3571 / liver cancer [ icgc ] DOID:0070003 / blastoma [ cosmic ]
K857 T857 Biomuta DOID:363 / uterine cancer [ tcga ]
K857 N857 Biomuta DOID:363 / uterine cancer [ tcga ]
K921 N921 Biomuta DOID:9256 / colorectal cancer [ icgc, tcga ] DOID:1909 / melanoma [ cosmic ]
K950 N950 Biomuta DOID:263 / kidney cancer [ cosmic, icgc, tcga ]
S1100 C1100 Biomuta DOID:1324 / lung cancer [ cosmic, icgc, tcga ]
S1100 F1100 Biomuta DOID:3070 / malignant glioma [ tcga ]
S1105 G1105 Biomuta DOID:1324 / lung cancer [ cosmic ]
T1112 P1112 Biomuta DOID:1324 / lung cancer [ cosmic ]
Y1153 C1153 Biomuta DOID:3963 / thyroid carcinoma [ cosmic, icgc ]
T1180 N1180 Biomuta DOID:3571 / liver cancer [ icgc ] DOID:0070003 / blastoma [ cosmic ]
S1341 F1341 Biomuta DOID:10283 / prostate cancer [ cosmic ]