iPTMnet Report P45985 MAP2K4

Protein Information

UniProt AC / UniProt ID	P45985 / MP2K4_HUMAN
Protein Name	Dual specificity mitogen-activated protein kinase kinase 4
Gene Name	Name: MAP2K4 Synonyms:JNKK1,MEK4,MKK4,PRKMK4,SEK1,SERK1,SKK1;
Organism	Homo sapiens (Human)

PRO ID	PR:P45985 DAG View
PRO Name	dual specificity mitogen-activated protein kinase kinase 4 (human)
Definition	A dual specificity mitogen-activated protein kinase kinase 4 that is encoded in the genome of human.
Short Label	hMAP2K4
Category	organism-gene

Interactive Sequence View

	Select/align proteoforms across species

P45985 (MAP2K4) as Substrate

Condensed View

Site	PTM Type	PTM Enzyme	Score	Source	PMID
A2	Acetylation		score1	UniProt	19413330
S5	Phosphorylation		score1	PhosphoSitePlus
S7	O-Glycosylation		score1	GlyGen	29351928
S7	Phosphorylation		score1	PhosphoSitePlus
S18	Phosphorylation		score1	PhosphoSitePlus
S26	Phosphorylation		score1	PhosphoSitePlus
S36	O-Glycosylation		score1	GlyGen	29351928
K42	Methylation		score1	PhosphoSitePlus
K45	Methylation		score1	PhosphoSitePlus
K54	Methylation		score1	PhosphoSitePlus
T56	Phosphorylation		score1	PhosphoSitePlus
R58	Methylation		score2	PhosphoSitePlus UniProt	24129315
T78	Phosphorylation		score1	PhosphoSitePlus
S80	Phosphorylation		score4	neXtProt phospho.ELM PhosphoSitePlus RLIMS-P Signor	20589745, 16000309, 22276160, ...17568772, 11707464, 15998799, 30680530, 25825522, 17276500, 15911620
S80	Phosphorylation	O00141 (SGK1)	score4	neXtProt PhosphoSitePlus	17568772, 25825522
S80	Phosphorylation	P31749 (AKT1)	score4	neXtProt PhosphoSitePlus Signor	11707464, 15911620
S83	Phosphorylation		score0	RLIMS-P	15911620
K86	Ubiquitination		score1	PhosphoSitePlus
K88	Ubiquitination		score1	PhosphoSitePlus
S90	Phosphorylation		score2	phospho.ELM PhosphoSitePlus UniProt	18669648
K103	Ubiquitination		score1	PhosphoSitePlus
K131	Acetylation		score1	PhosphoSitePlus
K131	Ubiquitination		score1	PhosphoSitePlus
K140	Ubiquitination		score1	PhosphoSitePlus
K143	Ubiquitination		score1	PhosphoSitePlus
K214	Ubiquitination		score1	PhosphoSitePlus
K231	Ubiquitination		score1	PhosphoSitePlus
S257	Phosphorylation		score4	neXtProt phospho.ELM PRO PhosphoSitePlus RLIMS-P Signor UniProt	19369195, 23157661, 26028649, ...33028008, 33101607, 30166526, 22539006, 24275569, 23755811, 9003778, 9162092, 15328343
S257	Phosphorylation	P45985 (MAP2K4)	score3	neXtProt phospho.ELM PhosphoSitePlus	9162092
S257	Phosphorylation	Q16584 (MAP3K11)	score4	neXtProt PhosphoSitePlus Signor	9003778, 15328343
T261	Phosphorylation		score4	neXtProt phospho.ELM PRO PhosphoSitePlus RLIMS-P Signor UniProt	33028008, 23157661, 20589745, ...26028649, 16000309, 33101607, 10727406, 30166526, 11053428, 23755811, 9003778, 8974401, 9712898, 9162092, 9182538, 15911620
T261	Phosphorylation	P45985 (MAP2K4)	score3	neXtProt phospho.ELM PhosphoSitePlus	9162092
T261	Phosphorylation	Q02779 (MAP3K10)	score2	neXtProt	9182538
T261	Phosphorylation	Q13233 (MAP3K1)	score1	Signor	9712898
T261	Phosphorylation	Q16584 (MAP3K11)	score4	neXtProt phospho.ELM PhosphoSitePlus Signor	11053428, 9003778, 10727406
T261	Phosphorylation	Q99683 (MAP3K5)	score4	phospho.ELM PhosphoSitePlus Signor	8974401, 15911620
Y269	Phosphorylation		score1	PhosphoSitePlus
Y307	Phosphorylation		score1	PhosphoSitePlus
T391	Phosphorylation		score1	PhosphoSitePlus
S393	Phosphorylation		score1	PhosphoSitePlus
S394	Phosphorylation		score1	PhosphoSitePlus

Site	PTM Type	PTM Enzyme	Score	Source	PMID
S5	Phosphorylation		score0	HPRD	20068231
S26	Phosphorylation		score0	HPRD	19413330, 20068231
S36	Phosphorylation		score0	HPRD	20068231
S80	Phosphorylation		score1	HPRD	11707464
S80	Phosphorylation	P31749 (AKT1)	score1	HPRD	11707464
S90	Phosphorylation		score0	HPRD	18669648
S257	Phosphorylation		score1	HPRD	9162092
S257	Phosphorylation	P45985 (MAP2K4)	score1	HPRD	9162092
T261	Phosphorylation		score1	HPRD	9162092
T261	Phosphorylation	P45985 (MAP2K4)	score1	HPRD	9162092
T391	Phosphorylation		score0	HPRD	20068231
S393	Phosphorylation		score0	HPRD	19413330
S394	Phosphorylation		score0	HPRD	19413330, 20068231

P45985 (MAP2K4) as PTM Enzyme

Protein as Phosphorylation Enzyme

Substrate	Site	Score	Source	PMID
P04637 (TP53)	S33	score2	RLIMS-P+	22730327
P19793 (RXRA)	Y249	score3	phospho.ELM PhosphoSitePlus Signor	10938283
P45983 (MAPK8)	T183	score4	neXtProt PhosphoSitePlus Signor	7839144, 9724739, 23416355, ...11062067
P45983 (MAPK8)	Y185	score4	HPRD neXtProt phospho.ELM PhosphoSitePlus Signor	7839144, 9724739, 23416355, ...18669648, 16153436, 11062067, 15911620
P45983-3 (MAPK8)	T228	score2	PhosphoSitePlus	23416355
P45983-3 (MAPK8)	S284	score2	PhosphoSitePlus	23416355
P45983-3 (MAPK8)	S377	score2	PhosphoSitePlus	23416355
P45984 (MAPK9)	T183	score1	Signor	17761173
P45984 (MAPK9)	Y185	score4	HPRD neXtProt phospho.ELM PhosphoSitePlus Signor	15911620, 22730327, 11062067
P45985 (MAP2K4)	S257	score3	HPRD neXtProt phospho.ELM PhosphoSitePlus	9162092
P45985-1 (MAP2K4)	T261	score3	HPRD neXtProt phospho.ELM PhosphoSitePlus	9162092
P53778 (MAPK12)	T183	score2	neXtProt	11062067
P53778 (MAPK12)	Y185	score4	HPRD neXtProt phospho.ELM	19413330, 11062067
P53779-3 (MAPK10)	T183	score2	HPRD	19651622, 10715136
P53779-3 (MAPK10)	Y185	score2	HPRD	19651622, 10715136
P53779-1 (MAPK10)	T221	score3	HPRD Signor	19651622, 10715136, 17761173
P53779 (MAPK10)	Y223	score4	HPRD neXtProt phospho.ELM PhosphoSitePlus Signor	19651622, 10715136, 11062067, ...15911620
Q16539 (MAPK14)	T180	score4	neXtProt PhosphoSitePlus Signor	7839144, 7535770
Q16539 (MAPK14)	Y182	score3	neXtProt PhosphoSitePlus	7839144

Proteoforms

	PRO ID (Short Label)	Sites	PTM Enzyme	Source	PMID
	PR:000048298 (hMAP2K4/Phos:1)	pS257,pT261		PRO

PTM sites affected in variants

Site	Variant	Source	Disease [Sample source]
S18	R18	Biomuta	DOID:2531 / hematologic cancer [ cosmic ]
S18	G18	Biomuta	DOID:1612 / breast cancer [ cosmic ]
K42	*	Biomuta	DOID:1781 / thyroid gland cancer [ cosmic ]
K45	*	Biomuta	DOID:4159 / skin cancer [ cosmic ]
R58	S58	Biomuta	DOID:10283 / prostate cancer [ cosmic, icgc ]
S80	N80	Biomuta	DOID:9256 / colorectal cancer [ cosmic, icgc ]
S83	A83	Biomuta	DOID:1793 / pancreatic cancer [ icgc ]
S83	*	Biomuta	DOID:9256 / colorectal cancer [ cosmic ]
S90	F90	Biomuta	DOID:4159 / skin cancer [ cosmic, icgc ]
K131	R131	Biomuta	DOID:9256 / colorectal cancer [ cosmic ]
K131	E131	Biomuta	DOID:4606 / bile duct cancer [ cosmic ]
K140	Q140	Biomuta	DOID:9256 / colorectal cancer [ cosmic ]
K143	T143	Biomuta	DOID:9256 / colorectal cancer [ cosmic ]
K231	I231	Biomuta	DOID:1612 / breast cancer [ cosmic, icgc ]
S257	Y257	Biomuta	DOID:5041 / esophageal cancer [ cosmic ]
S257	T257	Biomuta	DOID:9256 / colorectal cancer [ cosmic ]
S257	F257	Biomuta	DOID:4159 / skin cancer [ cosmic, icgc ] DOID:1612 / breast cancer [ cosmic, icgc ] DOID:9256 / colorectal cancer [ cosmic ]
S257	C257	Biomuta	DOID:1612 / breast cancer [ cosmic ]
T261	R261	Biomuta	DOID:10534 / stomach cancer [ cosmic ]
Y269	H269	Biomuta	DOID:9256 / colorectal cancer [ cosmic ]
Y269	C269	Biomuta	DOID:9256 / colorectal cancer [ cosmic ]

PTM Type		Select All, None
Acetylation	N-Glycosylation	O-Glycosylation
C-Glycosylation	S-Glycosylation	Methylation
Myristoylation	Phosphorylation	Sumoylation
Ubiquitination	S-Nitrosylation

Select organisms		Select All, None
Human	Mouse	Rat
Cow	Chicken	Zebrafish
Fruit fly	C. elegans	Baker's yeast
Fission yeast	A. thaliana	Maize
M. truncatula	Rice (japonica)	Rice (indica)

Or input other organisms

iPTMnet Report for P45985 (MAP2K4)

Protein Information

Interactive Sequence View

P45985 (MAP2K4) as Substrate

P45985 (MAP2K4) as PTM Enzyme

Proteoforms

PTM sites affected in variants