iPTMnet Report P43403 ZAP70

Protein Information

UniProt AC / UniProt ID	P43403 / ZAP70_HUMAN
Protein Name	Tyrosine-protein kinase ZAP-70
Gene Name	Name: ZAP70 Synonyms:SRK;
Organism	Homo sapiens (Human)

PRO ID	PR:P43403 DAG View
PRO Name	tyrosine-protein kinase ZAP-70 (human)
Definition	A tyrosine-protein kinase ZAP-70 that is encoded in the genome of human.
Short Label	hZAP70
Category	organism-gene

Interactive Sequence View

	Select/align proteoforms across species

P43403 (ZAP70) as Substrate

Condensed View

Site	PTM Type	PTM Enzyme	Score	Source	PMID
Y12	Phosphorylation		score1	PhosphoSitePlus
K25	Ubiquitination		score1	PhosphoSitePlus
Y46	Phosphorylation		score1	PhosphoSitePlus
Y69	Phosphorylation		score4	PhosphoSitePlus RLIMS-P Signor	7961936, 23874979
Y69	Phosphorylation	P06239 (LCK)	score3	PhosphoSitePlus Signor	7961936
K75	Ubiquitination		score1	PhosphoSitePlus
Y87	Phosphorylation		score1	PhosphoSitePlus
K100	Ubiquitination		score1	PhosphoSitePlus
S106	Phosphorylation		score1	PhosphoSitePlus
Y126	Phosphorylation		score4	neXtProt phospho.ELM PhosphoSitePlus RLIMS-P Signor	8756661, 10037717, 7961936
Y126	Phosphorylation	P06239 (LCK)	score2	PhosphoSitePlus	7961936
Y126	Phosphorylation	P43403 (ZAP70)	score4	neXtProt phospho.ELM PhosphoSitePlus Signor	8756661, 10037717, 7961936
K132	Ubiquitination		score1	PhosphoSitePlus
K151	Ubiquitination		score1	PhosphoSitePlus
Y164	Phosphorylation		score1	PhosphoSitePlus
S166	Phosphorylation		score1	PhosphoSitePlus
S167	Phosphorylation		score1	PhosphoSitePlus
K176	Ubiquitination		score1	PhosphoSitePlus
Y178	Phosphorylation		score3	PhosphoSitePlus RLIMS-P Signor	7961936
Y178	Phosphorylation	P06239 (LCK)	score3	PhosphoSitePlus Signor	7961936
S179	Phosphorylation		score1	PhosphoSitePlus
K186	Ubiquitination		score1	PhosphoSitePlus
K193	Ubiquitination		score1	PhosphoSitePlus
T197	Phosphorylation		score1	PhosphoSitePlus
Y198	Phosphorylation		score1	PhosphoSitePlus
Y204	Phosphorylation		score1	PhosphoSitePlus
K206	Ubiquitination		score1	PhosphoSitePlus
Y209	Phosphorylation		score1	PhosphoSitePlus
Y211	Phosphorylation		score1	PhosphoSitePlus
K217	Ubiquitination		score1	PhosphoSitePlus
K220	Ubiquitination		score1	PhosphoSitePlus
Y221	Phosphorylation		score1	PhosphoSitePlus
K242	Ubiquitination		score1	PhosphoSitePlus
Y248	Phosphorylation		score2	phospho.ELM PhosphoSitePlus UniProt	15592455, 15659558, 17192257
K251	Ubiquitination		score1	PhosphoSitePlus
S257	Phosphorylation		score1	PhosphoSitePlus
S258	Phosphorylation		score1	PhosphoSitePlus
S263	Phosphorylation		score1	PhosphoSitePlus
T278	Phosphorylation		score1	PhosphoSitePlus
T286	Phosphorylation		score1	PhosphoSitePlus
S289	Phosphorylation		score2	phospho.ELM PhosphoSitePlus UniProt	15144186
Y292	Phosphorylation		score4	neXtProt phospho.ELM PhosphoSitePlus RLIMS-P Signor UniProt	18548611, 7961936, 8943331, ...16094384, 25682964, 10779765, 23874979, 15144186, 15592455, 10037717, 9407100, 8756661, 9199177, 15659558, 26237552, 19690332, 17192257
Y292	Phosphorylation	P06239 (LCK)	score4	phospho.ELM PhosphoSitePlus	8756661, 26237552, 7961936
Y292	Phosphorylation	P43403 (ZAP70)	score4	neXtProt phospho.ELM PhosphoSitePlus Signor	8756661, 10037717, 7961936
T293	Phosphorylation		score3	PhosphoSitePlus RLIMS-P	29440413
T300	Phosphorylation		score1	PhosphoSitePlus
S301	Phosphorylation		score1	PhosphoSitePlus
K304	Ubiquitination		score1	PhosphoSitePlus
T312	Phosphorylation		score1	PhosphoSitePlus
S313	Phosphorylation		score1	PhosphoSitePlus
Y315	Phosphorylation		score4	IntAct neXtProt phospho.ELM PhosphoSitePlus RLIMS-P Signor UniProt	30356330, 7798261, 16094384, ...16339550, 18779373, 11514606, 30692994, 9151714, 8756661, 10037717, 21103055, 11828374, 15592455, 12522270, 32974219, 26237552
Y315	Phosphorylation	P06239 (LCK)	score2	PhosphoSitePlus	26237552
Y315	Phosphorylation	P43403 (ZAP70)	score4	neXtProt phospho.ELM PhosphoSitePlus Signor	8756661, 10037717, 7798261, ...11828374
S317	Phosphorylation		score1	PhosphoSitePlus
Y319	Phosphorylation		score4	IntAct neXtProt phospho.ELM PhosphoSitePlus RLIMS-P Signor UniProt	29915297, 10318843, 33482200, ...11514606, 15996927, 32974219, 18378687, 26783323, 17942891, 12522270, 33225946, 24256567, 27210755, 10202147, 15292262, 33917227, 21103055, 22665519, 23758320, 30356330, 20682913, 17960665, 15337788, 8756661, 36078131, 10037717, 15592455, 29456532, 26237552, 16094384, 17250586, 15268851, 11970985
Y319	Phosphorylation	P00519 (ABL1)	score3	neXtProt PhosphoSitePlus	15268851
Y319	Phosphorylation	P06239 (LCK)	score4	neXtProt PhosphoSitePlus	10202147, 26237552
Y319	Phosphorylation	P43403 (ZAP70)	score4	neXtProt PhosphoSitePlus Signor	8756661, 10037717
S320	Phosphorylation		score1	PhosphoSitePlus
K326	Ubiquitination		score1	PhosphoSitePlus
K329	Ubiquitination		score1	PhosphoSitePlus
S351	Phosphorylation		score1	PhosphoSitePlus
K361	Ubiquitination		score1	PhosphoSitePlus
K362	Ubiquitination		score1	PhosphoSitePlus
K369	Ubiquitination		score1	PhosphoSitePlus
K372	Ubiquitination		score1	PhosphoSitePlus
K377	Ubiquitination		score1	PhosphoSitePlus
Y397	Phosphorylation		score3	PhosphoSitePlus RLIMS-P	23874979
K450	Ubiquitination		score1	PhosphoSitePlus
Y451	Phosphorylation		score1	PhosphoSitePlus
K455	Ubiquitination		score1	PhosphoSitePlus
Y474	Phosphorylation		score3	phospho.ELM PhosphoSitePlus Signor	9685404
Y474	Phosphorylation	P06239 (LCK)	score3	phospho.ELM PhosphoSitePlus Signor	9685404
K476	Ubiquitination		score1	PhosphoSitePlus
K484	Ubiquitination		score1	PhosphoSitePlus
S491	Phosphorylation		score1	PhosphoSitePlus
Y492	Phosphorylation		score4	neXtProt phospho.ELM PhosphoSitePlus RLIMS-P Signor UniProt	7961936, 16094384, 16049944, ...17192257, 23874979, 8756661, 15592455, 7642520, 12522270, 9199177, 23620790, 7781602, 11270871, 26237552, 15659558
Y492	Phosphorylation	P06239 (LCK)	score4	neXtProt phospho.ELM PhosphoSitePlus Signor	7961936, 8756661, 7642520, ...7781602, 11270871, 26237552
Y492	Phosphorylation	P43403 (ZAP70)	score4	phospho.ELM PhosphoSitePlus Signor	8756661, 11270871, 7642520, ...7961936, 16049944
Y493	Phosphorylation		score4	neXtProt phospho.ELM PhosphoSitePlus RLIMS-P Signor UniProt	18548611, 15659558, 33482200, ...11514606, 23874979, 26783323, 12522270, 7961936, 21103055, 7781602, 11270871, 21757751, 23758320, 30356330, 15144186, 8756661, 15592455, 26237552, 7642520, 11976341, 16094384, 16049944, 27253329, 9199177, 24493801
Y493	Phosphorylation	P06239 (LCK)	score4	neXtProt phospho.ELM PhosphoSitePlus Signor	7961936, 8756661, 7642520, ...7781602, 11270871, 26237552
Y493	Phosphorylation	P43403 (ZAP70)	score4	phospho.ELM PhosphoSitePlus Signor	8756661, 11270871, 7642520, ...7961936, 16049944
T494	Phosphorylation		score1	PhosphoSitePlus
S497	O-Glycosylation		score1	GlyGen	27655845, 29351928
S497	Phosphorylation		score1	PhosphoSitePlus
K500	Ubiquitination		score1	PhosphoSitePlus
K504	Ubiquitination		score1	PhosphoSitePlus
Y506	Phosphorylation		score1	PhosphoSitePlus
S520	Phosphorylation		score3	PhosphoSitePlus RLIMS-P	14560012
S534	Phosphorylation		score1	PhosphoSitePlus
Y535	Phosphorylation		score1	PhosphoSitePlus
K538	Ubiquitination		score1	PhosphoSitePlus
K544	Ubiquitination		score3	PhosphoSitePlus UniProt	26903241
K556	Ubiquitination		score1	PhosphoSitePlus
Y569	Phosphorylation		score1	PhosphoSitePlus
Y597	Phosphorylation		score1	PhosphoSitePlus
Y598	Phosphorylation		score3	PhosphoSitePlus RLIMS-P	23874979
S599	Phosphorylation		score1	PhosphoSitePlus
K603	Acetylation		score2	PhosphoSitePlus UniProt	19608861
K603	Ubiquitination		score1	PhosphoSitePlus
T611	Phosphorylation		score1	PhosphoSitePlus
K613	Ubiquitination		score1	PhosphoSitePlus

Site	PTM Type	PTM Enzyme	Score	Source	PMID
Y69	Phosphorylation		score1	HPRD	7961936
Y69	Phosphorylation	P06239 (LCK)	score1	HPRD	7961936
Y126	Phosphorylation		score1	HPRD	7961936
Y126	Phosphorylation	P43403 (ZAP70)	score1	HPRD	7961936
Y178	Phosphorylation		score1	HPRD	7961936
Y178	Phosphorylation	P06239 (LCK)	score1	HPRD	7961936
Y248	Phosphorylation		score0	HPRD	15592455
S289	Phosphorylation		score0	HPRD	15144186
Y292	Phosphorylation		score2	HPRD	19901323, 7961936, 15144186
Y292	Phosphorylation	P43403 (ZAP70)	score2	HPRD	19901323, 7961936, 15144186
T293	Phosphorylation		score0	HPRD	15144186
Y315	Phosphorylation		score3	HPRD PRO	10037717
Y315	Phosphorylation	P43403 (ZAP70)	score1	HPRD	10037717
Y319	Phosphorylation		score2	HPRD	10318843, 7961936
Y319	Phosphorylation	P06239 (LCK)	score1	HPRD	10318843
Y319	Phosphorylation	P43403 (ZAP70)	score1	HPRD	7961936
Y474	Phosphorylation		score2	HPRD	7961936, 9685404
Y474	Phosphorylation	P06239 (LCK)	score2	HPRD	7961936, 9685404
Y492	Phosphorylation		score1	HPRD	7961936
Y492	Phosphorylation	P06239 (LCK)	score1	HPRD	7961936
Y493	Phosphorylation		score4	HPRD PRO	7961936, 15144186
Y493	Phosphorylation	P06239 (LCK)	score2	HPRD	7961936, 15144186
T494	Phosphorylation		score0	HPRD	15144186

Site	PTM Type	PTM Enzyme	Score	Source	PMID
Y8	Phosphorylation		score1	HPRD	10037717
Y8	Phosphorylation	P43403 (ZAP70)	score1	HPRD	10037717
Y12	Phosphorylation		score2	HPRD	10318843, 7961936
Y12	Phosphorylation	P06239 (LCK)	score1	HPRD	10318843
Y12	Phosphorylation	P43403 (ZAP70)	score1	HPRD	7961936
Y167	Phosphorylation		score2	HPRD	7961936, 9685404
Y167	Phosphorylation	P06239 (LCK)	score2	HPRD	7961936, 9685404
Y167	Phosphorylation	P29353 (SHC1)	score1	HPRD	7961936
Y185	Phosphorylation		score1	HPRD	7961936
Y185	Phosphorylation	P06239 (LCK)	score1	HPRD	7961936
Y186	Phosphorylation		score2	HPRD	7961936, 15144186
Y186	Phosphorylation	P06239 (LCK)	score2	HPRD	7961936, 15144186
T187	Phosphorylation		score0	HPRD	15144186

P43403 (ZAP70) as PTM Enzyme

Protein as Phosphorylation Enzyme

Substrate	Site	Score	Source	PMID
O43561-4 (LAT)	Y126	score1	HPRD	11368773
O43561-2 (LAT)	Y127	score3	HPRD neXtProt PhosphoSitePlus	11368773
O43561-4 (LAT)	Y131	score1	HPRD	11368773
O43561-2 (LAT)	Y132	score3	HPRD neXtProt PhosphoSitePlus	11368773
O43561 (LAT)	Y156	score2	HPRD Signor	11368773
O43561 (LAT)	Y161	score2	HPRD Signor	11368773
O43561-3 (LAT)	Y163	score1	HPRD	11368773
O43561-3 (LAT)	Y168	score1	HPRD	11368773
O43561-4 (LAT)	Y170	score1	HPRD	11368773
O43561-2 (LAT)	Y171	score3	HPRD neXtProt PhosphoSitePlus	11368773
O43561-4 (LAT)	Y190	score1	HPRD	11368773
O43561-2 (LAT)	Y191	score3	HPRD neXtProt PhosphoSitePlus	11368773
O43561 (LAT)	Y200	score2	HPRD Signor	11368773
O43561-3 (LAT)	Y207	score1	HPRD	11368773
O43561-1 (LAT)	Y220	score2	HPRD Signor	11368773
O43561-4 (LAT)	Y225	score1	HPRD	11368773
O43561-2 (LAT)	Y226	score3	HPRD neXtProt PhosphoSitePlus	11368773
O43561-3 (LAT)	Y227	score1	HPRD	11368773
O43561 (LAT)	Y255	score2	HPRD Signor	11368773
O43561-3 (LAT)	Y262	score1	HPRD	11368773
P15941-7 (MUC1)	Y229	score1	HPRD	14766232
P15941-11 (MUC1)	Y238	score1	HPRD	14766232
P15941 (MUC1)	Y1203	score1	Signor	14766232
P19174 (PLCG1)	Y783	score2	neXtProt	15161916
P19174 (PLCG1)	Y1253	score2	neXtProt	15161916
P29353-2 (SHC1)	Y239	score4	HPRD neXtProt PhosphoSitePlus	17016520, 19901323, 17389395, ...9710204, 10482988, 9388490, 16497976, 20068231, 9685404
P29353-2 (SHC1)	Y240	score4	HPRD neXtProt PhosphoSitePlus	19901323, 9388490, 17389395, ...9710204, 10482988, 9096689, 16497976, 20068231, 9685404
P29353-2 (SHC1)	Y317	score2	PhosphoSitePlus	9710204
P29353 (SHC1)	Y349	score4	HPRD phospho.ELM Signor	17016520, 19901323, 17389395, ...9710204, 15688026, 16497976, 20068231, 9685404
P29353 (SHC1)	Y350	score4	HPRD phospho.ELM Signor	19901323, 17389395, 9710204, ...15688026, 9685404, 20068231, 16497976
P29353-1 (SHC1)	Y427	score4	HPRD phospho.ELM Signor	18578522, 9710204, 9741627, ...10921922, 10482988, 10764799, 16497976, 20230923, 10896916, 18452278, 9121430, 12237775, 11075717, 17389395, 19651622, 17016520, 10816433, 19664994, 18669648, 9446569, 19901323, 20068231
P43403-2 (ZAP70)	Y8	score1	HPRD	10037717
P43403-2 (ZAP70)	Y12	score1	HPRD	7961936
P43403-1 (ZAP70)	Y126	score4	HPRD neXtProt phospho.ELM PhosphoSitePlus Signor	8756661, 10037717, 7961936
P43403 (ZAP70)	Y292	score4	HPRD neXtProt phospho.ELM PhosphoSitePlus Signor	8756661, 19901323, 10037717, ...7961936, 15144186
P43403-1 (ZAP70)	Y315	score4	HPRD neXtProt phospho.ELM PhosphoSitePlus Signor	8756661, 10037717, 7798261, ...11828374
P43403 (ZAP70)	Y319	score4	HPRD neXtProt PhosphoSitePlus Signor	8756661, 10037717, 7961936
P43403 (ZAP70)	Y492	score4	phospho.ELM PhosphoSitePlus Signor	8756661, 11270871, 7642520, ...7961936, 16049944
P43403 (ZAP70)	Y493	score4	phospho.ELM PhosphoSitePlus Signor	8756661, 11270871, 7642520, ...7961936, 16049944
P51452 (DUSP3)	Y38	score3	neXtProt PhosphoSitePlus	12447358
P51452 (DUSP3)	Y138	score3	HPRD neXtProt phospho.ELM PhosphoSitePlus Signor	12447358
Q13094 (LCP2)	Y113	score4	HPRD phospho.ELM PhosphoSitePlus Signor	8702662, 12817019, 10660534, ...9047237
Q13094 (LCP2)	Y128	score4	HPRD phospho.ELM PhosphoSitePlus Signor	8702662, 12817019, 10660534, ...9047237
Q13094 (LCP2)	Y145	score4	HPRD phospho.ELM PhosphoSitePlus Signor	8702662, 12817019, 8892604
Q13094 (LCP2)	Y423	score1	Signor	8702662
Q13094 (LCP2)	Y426	score1	Signor	8702662
Q16539 (MAPK14)	Y323	score3	neXtProt phospho.ELM PhosphoSitePlus Signor	15735648
Q8N1K5 (THEMIS)	Y540	score2	PhosphoSitePlus	23460737
Q8N1K5 (THEMIS)	Y541	score2	PhosphoSitePlus	23460737
Q9UJU6-1 (DBNL)	Y334	score3	HPRD neXtProt phospho.ELM PhosphoSitePlus Signor	14557276
Q9UJU6-2 (DBNL)	Y335	score1	HPRD	14557276
Q9UJU6 (DBNL)	Y344	score3	HPRD neXtProt phospho.ELM PhosphoSitePlus Signor	14557276
Q9UJU6-2 (DBNL)	Y345	score1	HPRD	14557276
Q9UQC2 (GAB2)	Y614	score3	HPRD phospho.ELM PhosphoSitePlus Signor	11572860
Q9UQQ2 (SH2B3)	Y273	score3	HPRD phospho.ELM Signor	9169414

Proteoforms

	PRO ID (Short Label)	Sites	PTM Enzyme	Source	PMID
	PR:000049722 (hZAP70/Phos:2)	pY315,pY493		PRO
	PR:000049723 (hZAP70/Phos:3)	pY493		PRO

PTM-dependent PPI

	PTM type	Substrate	Site	Interactant	Association type	Source	PMID
	Phosphorylation	P43403 (ZAP70)	Y319	P06239 (LCK)	unknown	eFIP	10318843

Proteoform PPIs

	Protein 1	Relation	Protein 2	Source	PMID
	PR:000036592 (hEZR/iso:1/Phos:5)	Interaction	PR:P43403 (hZAP70)	PRO	18025306

PTM sites affected in variants

Site	Variant	Source	Disease [Sample source]
K75	N75	Biomuta	DOID:9256 / colorectal cancer [ cosmic ]
K100	R100	Biomuta	DOID:10534 / stomach cancer [ cosmic ]
Y167	*	Biomuta	DOID:1324 / lung cancer [ cosmic ]
S179	F179	Biomuta	DOID:1324 / lung cancer [ cosmic, icgc ]
Y185	C185	Biomuta	DOID:1319 / brain cancer [ cosmic, icgc ]
Y209	H209	Biomuta	DOID:10534 / stomach cancer [ cosmic, icgc ]
Y211	*	Biomuta	DOID:1324 / lung cancer [ cosmic ]
K217	M217	Biomuta	DOID:9256 / colorectal cancer [ cosmic, icgc ]
S263	L263	Biomuta	DOID:1612 / breast cancer [ cosmic, icgc ]
T300	M300	Biomuta	DOID:9256 / colorectal cancer [ cosmic ] DOID:10534 / stomach cancer [ cosmic ]
S301	F301	Biomuta	DOID:4159 / skin cancer [ cosmic ]
T312	M312	Biomuta	DOID:10283 / prostate cancer [ cosmic ] DOID:5041 / esophageal cancer [ cosmic ]
K329	N329	Biomuta	DOID:8618 / oral cavity cancer [ cosmic ]
K362	N362	Biomuta	DOID:10283 / prostate cancer [ cosmic, icgc ]
K450	Q450	Biomuta	DOID:263 / kidney cancer [ cosmic ]
Y474	*	Biomuta	DOID:1324 / lung cancer [ cosmic ]
Y492	C492	Biomuta	DOID:1319 / brain cancer [ cosmic, icgc ]
K500	R500	Biomuta	DOID:10283 / prostate cancer [ cosmic ]
Y506	F506	Biomuta	DOID:4159 / skin cancer [ cosmic ]
Y506	H506	Biomuta	DOID:10534 / stomach cancer [ cosmic ]
S520	I520	Biomuta	DOID:1319 / brain cancer [ icgc ]

PTM Type		Select All, None
Acetylation	N-Glycosylation	O-Glycosylation
C-Glycosylation	S-Glycosylation	Methylation
Myristoylation	Phosphorylation	Sumoylation
Ubiquitination	S-Nitrosylation

Select organisms		Select All, None
Human	Mouse	Rat
Cow	Chicken	Zebrafish
Fruit fly	C. elegans	Baker's yeast
Fission yeast	A. thaliana	Maize
M. truncatula	Rice (japonica)	Rice (indica)

Or input other organisms

iPTMnet Report for P43403 (ZAP70)

Protein Information

Interactive Sequence View

P43403 (ZAP70) as Substrate

P43403 (ZAP70) as PTM Enzyme

Proteoforms

PTM-dependent PPI

Proteoform PPIs

PTM sites affected in variants