Protein Information

Cytoscape View


UniProt AC / UniProt ID P35568 / IRS1_HUMAN
Protein Name Insulin receptor substrate 1
Gene Name Name: IRS1
Organism Homo sapiens (Human)

PRO ID PR:P35568
DAG View
PRO Name insulin receptor substrate 1 (human)
Definition An insulin receptor substrate 1 that is encoded in the genome of human.
Short Label hIRS1
Category organism-gene

Interactive Sequence View

Select/align proteoforms across species


P35568 (IRS1) as Substrate



Site PTM Type PTM Enzyme Score Source PMID
Phosphorylation PRO
S3 Phosphorylation HPRD   PhosphoSitePlus 19651622
S24 Phosphorylation P53350 (PLK1) , P17252 (PRKCA) , P67870 (CSNK2B) HPRD   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor 15849359, 16970908, 16574739, ...19675138, 8349691, 25330241
Y46 Phosphorylation HPRD   phospho.ELM   PhosphoSitePlus 15592455, 18083107
Y47 Phosphorylation PhosphoSitePlus
K52 Acetylation PhosphoSitePlus
K61 Acetylation PhosphoSitePlus
Y87 Phosphorylation HPRD   PhosphoSitePlus 20058876
T88 Phosphorylation P67870 (CSNK2B) HPRD 8349691
S99 Phosphorylation P67870 (CSNK2B) HPRD 8349691
Y107 Phosphorylation PhosphoSitePlus
K169 Ubiquitination PhosphoSitePlus
Y183 Phosphorylation PhosphoSitePlus
S268 Phosphorylation O14920 (IKBKB) HPRD   phospho.ELM   PhosphoSitePlus 12351658
S270 Phosphorylation O14920 (IKBKB) , Q13535 (ATR) , P23443 (RPS6KB1) HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 12351658, 22348058, 28934129, ...18952604, 19651622, 9312143, 18077418
S272 Phosphorylation O14920 (IKBKB) HPRD   phospho.ELM 12351658
S273 Phosphorylation PhosphoSitePlus
S274 Phosphorylation O14920 (IKBKB) HPRD   phospho.ELM   PhosphoSitePlus 12351658
S277 Phosphorylation PhosphoSitePlus
S281 Phosphorylation PhosphoSitePlus
S303 Phosphorylation PhosphoSitePlus
T305 Phosphorylation PhosphoSitePlus
S307 Phosphorylation Q02750 (MAP2K1) , P45984 (MAPK9) , P23443 (RPS6KB1) , O14920 (IKBKB) , Q05655 (PRKCD) , P45983 (MAPK8) , P42345 (MTOR) HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P+   RLIMS-P   Signor   UniProt 22110065, 14697666, 20702573, ...12510059, 17575262, 26887316, 28866332, 21119656, 24846082, 22348058, 12730242, 22328823, 22266116, 24068326, 14583092, 22728372, 16210322, 15153564, 20594757, 29311680, 20541543, 18952604, 21900690, 23174405, 30287053, 25645159, 27506476, 25120613, 27222060, 26899161, 20351777, 27936435, 15199052, 19897715, 18187553, 15240096, 27544192, 31311314, 29177868, 28334990, 27686967, 30240640, 17276034, 23386514, 23565163, 30629257, 25355439, 20594161, 26604643, 29872751, 29036927, 23566306, 17481767, 25799226, 11606564, 23095254, 17077083, 14579029, 28656239, 9335553, 31396538, 15802620, 28934129, 23891856, 16754954, 22766331, 18380932, 27034691, 26698173, 29393871, 15516986, 29698741, 12714600, 16516141, 20382011, 14641015, 11160134, 15020250, 22046423, 28708100, 21505982, 16914728, 31137828, 21478152, 16055440, 17095576, 18372392, 22227580, 26704265, 18496818, 23481042, 14556646, 16129690, 30374127, 23958494, 15582605, 16839860, 16269459, 19524129, 22491740, 16046301, 23182460, 31223033, 12351658, 16099428, 18498745, 28790390, 19095745, 21786209, 21852229, 23592917, 12409308, 20384568, 28161417
T309 Phosphorylation PhosphoSitePlus
T311 Phosphorylation PhosphoSitePlus
S312 Phosphorylation P28482 (MAPK1) , O14733 (MAP2K7) , O14920 (IKBKB) , P45983 (MAPK8) HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 18160431, 15199052, 12510059, ...12351658, 22457223, 28334990, 17204522, 17360977, 29038536, 20685959, 22348058, 16505244, 19567513, 16516141, 21589939, 15613682, 18728222, 27340831, 19501568, 14769130, 15952059, 19593406, 17242212, 15001544, 18498745, 21252690, 20863384, 10722755, 16284649, 15044323, 15713122, 16960890, 16505239, 11606564, 27207509, 21937042, 17647275, 25580119, 16123357, 21852229, 22862962, 21040800
S315 Phosphorylation P45983 (MAPK8) , P45984 (MAPK9) PhosphoSitePlus   Signor 14579029, 12510059, 9335553
S323 Phosphorylation Q05655 (PRKCD) , P05771 (PRKCB) , Q05513 (PRKCZ) neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 16129678, 15516986, 16611834, ...14583092, 24275569, 15069075
S329 Phosphorylation PhosphoSitePlus
S330 Phosphorylation P67870 (CSNK2B) HPRD   PhosphoSitePlus 8349691
T335 Phosphorylation PhosphoSitePlus
S337 Phosphorylation RLIMS-P 23669880
S341 Phosphorylation O14920 (IKBKB) HPRD   phospho.ELM   PhosphoSitePlus 12351658
S345 Phosphorylation O14920 (IKBKB) HPRD   phospho.ELM   PhosphoSitePlus 12351658
S348 Phosphorylation phospho.ELM   PhosphoSitePlus   RLIMS-P   UniProt 16020478, 17360977, 19690332, ...18220336
S350 Phosphorylation PhosphoSitePlus
T351 Phosphorylation HPRD   PhosphoSitePlus 19664994
S362 Phosphorylation Q05655 (PRKCD) neXtProt   PhosphoSitePlus 18285345, 14583092
S372 Phosphorylation PhosphoSitePlus
R373 Methylation PhosphoSitePlus
S374 Phosphorylation phospho.ELM   PhosphoSitePlus 16020478
S383 Phosphorylation PhosphoSitePlus
S385 Phosphorylation PhosphoSitePlus
S388 Phosphorylation PhosphoSitePlus
S391 Phosphorylation PhosphoSitePlus
S417 Phosphorylation PhosphoSitePlus
S419 Phosphorylation PhosphoSitePlus
S441 Phosphorylation Q05655 (PRKCD) , Q05513 (PRKCZ) , P05771 (PRKCB) PhosphoSitePlus 16611834
S444 Phosphorylation PhosphoSitePlus
T446 Phosphorylation PhosphoSitePlus   RLIMS-P 17360977
S449 Phosphorylation PhosphoSitePlus
T453 Phosphorylation PhosphoSitePlus   UniProt 24275569
S463 Phosphorylation PhosphoSitePlus
Y465 Phosphorylation P08069 (IGF1R) PhosphoSitePlus   RLIMS-P   Signor 17827393, 27434075
Y483 Phosphorylation PhosphoSitePlus
S486 Phosphorylation PhosphoSitePlus
T495 Phosphorylation PhosphoSitePlus   RLIMS-P 17360977
T498 Phosphorylation PhosphoSitePlus
T502 Phosphorylation P68400 (CSNK2A1) HPRD   PhosphoSitePlus 8349691
S503 Phosphorylation PhosphoSitePlus
T525 Phosphorylation PhosphoSitePlus
S527 Phosphorylation P23443 (RPS6KB1) , O14920 (IKBKB) HPRD   phospho.ELM   PhosphoSitePlus   Signor   UniProt 19651622, 12351658, 24275569, ...18498745, 16914728
T530 Phosphorylation phospho.ELM   PhosphoSitePlus 16020478
S531 Phosphorylation O14920 (IKBKB) HPRD   phospho.ELM   PhosphoSitePlus 19651622, 12351658, 18220336
T533 Phosphorylation PhosphoSitePlus
S574 Phosphorylation Q05655 (PRKCD) HPRD   phospho.ELM   PhosphoSitePlus   RLIMS-P 15516986, 14583092, 16131083
S581 Phosphorylation PhosphoSitePlus
S603 Phosphorylation PhosphoSitePlus
S604 Phosphorylation PhosphoSitePlus
T605 Phosphorylation PhosphoSitePlus
T608 Phosphorylation PhosphoSitePlus
Y612 Phosphorylation P23458 (JAK1) , P45984 (MAPK9) , P08069 (IGF1R) HPRD   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P   Signor 17827393, 28934129, 28414818, ...21939528, 28955049, 17902048, 31396538, 26874193, 28345670, 11162588, 16188907, 20150288, 23460259, 29259227, 17242212, 15001544, 28161417, 25302072, 23688189, 15044323, 29486856, 12220227, 26297026, 14579029, 18083107, 19762915, 21829658, 25886138
S616 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1) , P45983 (MAPK8) , Q05655 (PRKCD) HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor 17360977, 26297026, 17242212, ...21937042, 14579029, 15001544, 16131083, 17640984, 14769130, 12510059, 9395471, 22476197, 16354680, 15044323, 15143153, 15713122, 8626671
S624 Phosphorylation PhosphoSitePlus
S629 Phosphorylation P31749 (AKT1) HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   UniProt 17081983, 16131083, 17640984, ...19651622, 24275569
Y632 Phosphorylation P45984 (MAPK9) , P23458 (JAK1) , P08069 (IGF1R) HPRD   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor 17827393, 8530377, 22558377, ...11162588, 28934129, 29698741, 14579029, 15001544, 28414818, 17640984, 18083107, 30863203, 26077338, 17279354, 31137828, 15044323
S636 Phosphorylation P23443 (RPS6KB1) , O75116 (ROCK2) , P28482 (MAPK1) , P45983 (MAPK8) HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 25667991, 30374127, 28934129, ...19581421, 20203102, 26077338, 15576463, 12765939, 18597775, 17360977, 22348058, 16131083, 16099428, 25813876, 14769130, 16098829, 19561084, 30381640, 11287630, 28652303, 22966072, 18952604, 17640984, 19651622, 16284649, 16354680, 21478152, 27434075, 18498745, 25799226, 17081983, 23512499, 14579029, 16123357, 21829658, 12510059
S639 Phosphorylation P28482 (MAPK1) neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P 25667991, 25799226, 16354680, ...23512499, 19561084, 30381640, 11287630, 30374127, 17640984, 20203102, 15576463, 16123357, 16099428, 21829658, 21478152, 14769130
Y662 Phosphorylation P08069 (IGF1R) , P23458 (JAK1) HPRD   phospho.ELM   PRO   PhosphoSitePlus   UniProt 15592455, 11162588, 8530377
S666 Phosphorylation HPRD   PhosphoSitePlus 8626671
S668 Phosphorylation PhosphoSitePlus
S672 Phosphorylation PhosphoSitePlus
S680 Phosphorylation PhosphoSitePlus
S694 Phosphorylation PhosphoSitePlus
Y695 Phosphorylation PhosphoSitePlus
Y732 Phosphorylation P23458 (JAK1) , P08069 (IGF1R) HPRD   PRO   PhosphoSitePlus 11162588, 8530377
S736 Phosphorylation HPRD 8626671
S766 Phosphorylation HPRD   PhosphoSitePlus 19664994
K772 Methylation PhosphoSitePlus
K772 Ubiquitination PhosphoSitePlus
S794 Phosphorylation Q9H0K1 (SIK2) HPRD   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 20135346, 31446747, 12006586, ...20363874, 20863384, 12624099, 17459875
Y800 Phosphorylation PhosphoSitePlus
T811 Phosphorylation P67870 (CSNK2B) HPRD 8349691
T847 Phosphorylation PhosphoSitePlus
T859 Phosphorylation PhosphoSitePlus
S862 Phosphorylation PhosphoSitePlus
K867 Ubiquitination PhosphoSitePlus
S892 Phosphorylation phospho.ELM   PhosphoSitePlus   RLIMS-P 16020478, 17360977
Y896 Phosphorylation P08069 (IGF1R) , P06241 (FYN) , P06213 (INSR) HPRD   neXtProt   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P   Signor 21939528, 17827393, 12220227, ...17902048, 8631859, 23983791, 11259577, 8530377, 25886138
T936 Phosphorylation PhosphoSitePlus
T938 Phosphorylation PhosphoSitePlus
Y941 Phosphorylation P08069 (IGF1R) HPRD   PRO   PhosphoSitePlus   RLIMS-P   Signor   UniProt 22617042, 17827393, 12220227, ...20685959, 25299696, 26378251, 8530377
S974 Phosphorylation O75116 (ROCK2) neXtProt 16098829
S984 O-Glycosylation PhosphoSitePlus
S984 Phosphorylation RLIMS-P 25580119
S985 O-Glycosylation PhosphoSitePlus
Y989 Phosphorylation P08069 (IGF1R) , P06213 (INSR) PhosphoSitePlus   RLIMS-P   Signor 22709426, 17827393, 23045700, ...8530377
S1005 Phosphorylation PhosphoSitePlus   RLIMS-P 17360977
S1011 O-Glycosylation PhosphoSitePlus
Y1012 Phosphorylation PhosphoSitePlus
S1025 Phosphorylation PhosphoSitePlus
S1037 Phosphorylation RLIMS-P 25580119
S1041 Phosphorylation PhosphoSitePlus
S1043 Phosphorylation PhosphoSitePlus
T1045 Phosphorylation RLIMS-P 16244361
S1078 Phosphorylation phospho.ELM   PhosphoSitePlus 16503154
S1084 Phosphorylation PhosphoSitePlus
S1100 Phosphorylation P17612 (PRKACA) HPRD   PhosphoSitePlus   Signor 19651622, 17360977
S1101 Phosphorylation P05129 (PRKCG) , Q05513 (PRKCZ) , Q04759 (PRKCQ) , Q05655 (PRKCD) , P23443 (RPS6KB1) HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 17709744, 23352416, 24036112, ...17360977, 24810050, 18220336, 17647275, 22046423, 28934129, 18952604, 20203102, 25580119, 16914728, 26298773, 28674284, 15364919, 24979806, 30374127, 17081983
T1103 Phosphorylation HPRD   PhosphoSitePlus 17081983
S1105 Phosphorylation PhosphoSitePlus
T1107 Phosphorylation PhosphoSitePlus
S1109 Phosphorylation PhosphoSitePlus
T1111 Phosphorylation phospho.ELM   PhosphoSitePlus 18220336
T1116 Phosphorylation PhosphoSitePlus
S1142 Phosphorylation PhosphoSitePlus
S1143 Phosphorylation HPRD   PhosphoSitePlus 17081983
S1145 Phosphorylation HPRD   phospho.ELM   PhosphoSitePlus 16020478, 17081983
Y1179 Phosphorylation P06213 (INSR) , P08069 (IGF1R) , P06241 (FYN) HPRD   neXtProt   PRO   PhosphoSitePlus   Signor 17827393, 8631859, 9756938, ...8530377
S1222 Phosphorylation P17612 (PRKACA) phospho.ELM   PhosphoSitePlus   Signor 16020478, 17360977
S1223 Phosphorylation P17612 (PRKACA) HPRD   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor 16020478, 17360977, 16131083
Y1229 Phosphorylation P06213 (INSR) , P08069 (IGF1R) HPRD   RLIMS-P   Signor 17827393, 9756938, 12220227


Proteoforms

PRO ID (Short Label) Sites PTM Enzyme Source PMID
PR:000048051 (hIRS1/Phos:2) pY612,pY662,pY732,pY896,pY941,pY1179 PRO
PR:000048052 (hIRS1/PhosTyr+) p PRO


PTM-dependent PPI

PTM type Substrate Site Interactant Association type Source PMID
Phosphorylation P35568 (IRS1) Y612 P00533 (EGFR) increased_association eFIP 17902048
Phosphorylation P35568 (IRS1) Y612 P08069 (IGF1R) increased_association eFIP 17902048
Phosphorylation P35568 (IRS1) S1223 Q06124 (PTPN11) unknown eFIP 16020478
Phosphorylation P35568 (IRS1) S636 O00329 (PIK3CD) decreased_association eFIP 23604550
Phosphorylation P35568 (IRS1) S312 O00459 (PIK3R2) unknown eFIP 16505239


PTM sites affected in variants

Site Variant Source PMID Disease [Sample source]
S24 N24 Biomuta DOID:3070 / malignant glioma [ tcga ]
Y46 H46 Biomuta DOID:10534 / stomach cancer [ cosmic, icgc, tcga ]
Y46 * Biomuta DOID:1612 / breast cancer [ tcga ]
Y47 C47 Biomuta DOID:3070 / malignant glioma [ cosmic ]
T88 A88 Biomuta DOID:11054 / urinary bladder cancer [ cosmic, icgc ]
S270 G270 Biomuta DOID:9256 / colorectal cancer [ tcga ]
S272 P272 Biomuta DOID:1319 / brain cancer [ icgc ]
S277 C277 Biomuta DOID:1612 / breast cancer [ tcga ]
S323 A323 Biomuta DOID:363 / uterine cancer [ cosmic, icgc, tcga ]
S330 N330 Biomuta DOID:363 / uterine cancer [ tcga ]
T335 I335 Biomuta DOID:1909 / melanoma [ icgc, tcga ]
R373 C373 Biomuta DOID:1324 / lung cancer [ cosmic, icgc, tcga ]
S383 L383 Biomuta DOID:1909 / melanoma [ cosmic ]
S385 * Biomuta DOID:1324 / lung cancer [ tcga ]
S385 L385 Biomuta DOID:9256 / colorectal cancer [ icgc, tcga ] DOID:1909 / melanoma [ cosmic ]
T495 A495 Biomuta DOID:10534 / stomach cancer [ cosmic ]
T498 I498 Biomuta DOID:1909 / melanoma [ icgc, tcga ] DOID:11934 / head and neck cancer [ tcga ]
S531 Y531 Biomuta DOID:3571 / liver cancer [ icgc ]
T533 I533 Biomuta DOID:10283 / prostate cancer [ cosmic ]
S581 Y581 Biomuta DOID:1324 / lung cancer [ cosmic, tcga ]
Y632 C632 Biomuta DOID:1324 / lung cancer [ cosmic ]
S672 C672 Biomuta DOID:1324 / lung cancer [ cosmic, tcga ]
Y732 * Biomuta DOID:3070 / malignant glioma [ cosmic, icgc, tcga ]
Y800 C800 Biomuta DOID:1324 / lung cancer [ cosmic ]
Y800 * Biomuta DOID:1909 / melanoma [ icgc, tcga ]
T811 A811 Biomuta DOID:10534 / stomach cancer [ tcga ]
S974 I974 Biomuta DOID:8618 / oral cavity cancer [ icgc ]
S1005 L1005 Biomuta DOID:363 / uterine cancer [ tcga ]
S1109 C1109 Biomuta DOID:1324 / lung cancer [ cosmic, tcga ]
S1223 G1223 Biomuta DOID:1909 / melanoma [ tcga ]