Protein Information

Cytoscape View


UniProt AC / UniProt ID O96017 / CHK2_HUMAN
Protein Name Serine/threonine-protein kinase Chk2
Gene Name Name: CHEK2
Synonyms:CDS1,CHK2,RAD53;
Organism Homo sapiens (Human)

PRO ID PR:O96017
DAG View
PRO Name serine/threonine-protein kinase Chk2 (human)
Definition A serine/threonine-protein kinase Chk2 that is encoded in the genome of human.
Short Label hCHEK2
Category organism-gene

Interactive Sequence View

Select/align proteoforms across species


O96017 (CHEK2) as Substrate



Site PTM Type PTM Enzyme Score Source PMID
S12 Phosphorylation PhosphoSitePlus
S15 Phosphorylation PhosphoSitePlus   RLIMS-P 20542099, 14970866, 12637545, ...15322239, 17653574, 17940507
S19 Phosphorylation O96017 (CHEK2) , Q13315 (ATM) phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor 19390600, 30273361, 25535901, ...17363488, 16940182, 10973490, 20713355, 22808196, 18812180, 18538787
S24 Phosphorylation PhosphoSitePlus
T26 Phosphorylation P53350 (PLK1) , Q13315 (ATM) phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor 12024051, 12493754, 10973490
S28 Phosphorylation Q13315 (ATM) phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor 10973490, 12493754, 12024051
S33 Phosphorylation O96017 (CHEK2) , Q13315 (ATM) phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor 25535901, 17363488, 19666469, ...20160490, 10973490, 20713355, 14695167, 16940182, 18538787
S35 Phosphorylation O96017 (CHEK2) , Q13315 (ATM) phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor 25535901, 17363488, 19666469, ...20160490, 10973490, 20713355, 14695167, 16940182, 18538787
S39 Phosphorylation PhosphoSitePlus
S40 Phosphorylation PhosphoSitePlus
S41 Phosphorylation PhosphoSitePlus
S42 Phosphorylation PhosphoSitePlus
T43 Phosphorylation PhosphoSitePlus
S44 Phosphorylation PhosphoSitePlus
T45 Phosphorylation PhosphoSitePlus
S50 Phosphorylation Q13315 (ATM) phospho.ELM   PhosphoSitePlus   Signor 10973490
S52 Phosphorylation PhosphoSitePlus
S55 Phosphorylation PhosphoSitePlus
S62 Phosphorylation Q9H4B4 (PLK3) neXtProt   PhosphoSitePlus   RLIMS-P   UniProt 16481012, 12242661, 21376736
T65 Phosphorylation PhosphoSitePlus
S67 Phosphorylation PhosphoSitePlus
T68 Phosphorylation P33981 (TTK) , P78527 (PRKDC) , Q13535 (ATR) , P53350 (PLK1) , Q683Z8 (CHK2) , Q9NYL2 (MAP3K20) , Q13315 (ATM) , O96017 (CHEK2) neXtProt   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P   Signor   UniProt 23690919, 31269698, 12493754, ...18948271, 24415942, 16481012, 15361851, 27888802, 11901158, 17940507, 16461339, 17384638, 22170260, 21310354, 25111195, 24553354, 27991505, 30337689, 17513615, 15048074, 21765476, 19798429, 19001859, 29340034, 12386164, 11085506, 15533933, 23383325, 20424615, 23977391, 20126263, 28049532, 24190430, 26441204, 22099307, 21338495, 15876876, 23272087, 29563987, 22623962, 29643855, 17671118, 24492479, 16794575, 12607004, 20637859, 15322239, 30272263, 15700044, 12024051, 21945437, 15342622, 26333122, 18985806, 12242661, 19658174, 20305300, 19451272, 18762365, 12637545, 16024600, 24153426, 24662822, 18957425, 26333361, 20713355, 28103869, 26041102, 20637980, 28967905, 25527123, 16705183, 15668230, 21102523, 22312557, 15618221, 23598415, 28259974, 26158765, 19390600, 25895498, 10744722, 28852018, 27001483, 24798733, 24069304, 24882580, 27125496, 15784679, 22589537, 25613496, 11390408, 16150728, 21390062, 26751069, 28539821, 15963507, 30245854, 23013471, 21772273, 28094252, 10973490, 17715138, 16940182, 27767095, 16596250, 16322218, 24792164, 19782031, 11025670, 19330022, 26416514, 19377277, 30900384, 21147465, 19151762, 15140942, 30473536, 29399146, 27029031, 23705030, 27076856, 20599567, 30483783, 20406977, 18644861, 25886177, 24844881, 29741650, 26542114, 12805407, 19411857, 16311512, 26919204, 25875766, 29868498, 16478990, 18812180, 24758542, 24498068, 15213307, 16456034, 25535901, 28038466, 17236549, 16741947, 11668173, 29444163, 30304835, 16798742, 26485758, 12447382, 30959320, 24812435, 11096068, 21624128, 17363488, 17685927, 23666059, 26442630, 14695167, 11053450
S73 Phosphorylation Q9H4B4 (PLK3) neXtProt   PhosphoSitePlus   RLIMS-P   UniProt 16481012, 12242661, 21376736
K119 Ubiquitination PhosphoSitePlus
S120 Phosphorylation O96017 (CHEK2) PhosphoSitePlus 18538787
K131 Ubiquitination PhosphoSitePlus
S140 Phosphorylation O96017 (CHEK2) PhosphoSitePlus 18948271
S164 Phosphorylation P53350 (PLK1) PhosphoSitePlus 20126263
T205 Phosphorylation P53350 (PLK1) PhosphoSitePlus   RLIMS-P 20126263
S210 Phosphorylation P53350 (PLK1) PhosphoSitePlus   RLIMS-P 20126263
K224 Ubiquitination PhosphoSitePlus
T225 Phosphorylation O96017 (CHEK2) PhosphoSitePlus 18538787
K235 Acetylation PhosphoSitePlus
S260 Phosphorylation O96017 (CHEK2) PhosphoSitePlus 18538787
K279 Ubiquitination PhosphoSitePlus
K287 Ubiquitination PhosphoSitePlus
S357 Phosphorylation Q13315 (ATM) neXtProt 10973490
S372 Phosphorylation PhosphoSitePlus   RLIMS-P 20713355
K373 Ubiquitination PhosphoSitePlus
T378 Phosphorylation PhosphoSitePlus   RLIMS-P 20713355
S379 Phosphorylation O96017 (CHEK2) neXtProt   PRO   PhosphoSitePlus   RLIMS-P   Signor   UniProt 18644861, 20713355, 18538787
T383 Phosphorylation P78527 (PRKDC) , O96017 (CHEK2) , Q683Z8 (CHK2) phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P   Signor   UniProt 11390408, 12805407, 18644861, ...24798733, 15668230, 11901158, 20713355
T387 Phosphorylation Q683Z8 (CHK2) , P78527 (PRKDC) , O96017 (CHEK2) phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P   Signor   UniProt 11390408, 16941491, 12805407, ...18644861, 15668230, 11901158, 15361830, 20713355, 26158765
T389 Phosphorylation PhosphoSitePlus   RLIMS-P 20713355
Y390 Phosphorylation PhosphoSitePlus   RLIMS-P 20713355
T432 Phosphorylation O96017 (CHEK2) PhosphoSitePlus 18538787
S435 Phosphorylation O96017 (CHEK2) PhosphoSitePlus 18538787
K437 Ubiquitination PhosphoSitePlus
K444 Ubiquitination PhosphoSitePlus
S456 Phosphorylation phospho.ELM   PhosphoSitePlus   RLIMS-P   UniProt 26751770, 17715138
K458 Ubiquitination PhosphoSitePlus
K472 Methylation PhosphoSitePlus
K472 Ubiquitination PhosphoSitePlus
K494 Ubiquitination PhosphoSitePlus
S516 Phosphorylation O96017 (CHEK2) , Q683Z8 (CHK2) phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor 21239475, 22004065, 12805407, ...18644861, 12855706, 23855452, 20713355, 27935866, 18538787
T517 Phosphorylation PhosphoSitePlus
C539 S-Nitrosylation dbSNO 26338901
Site PTM Type PTM Enzyme Score Source PMID
S19 Phosphorylation Q13315 (ATM) , Q13535 (ATR) HPRD 10973490
T26 Phosphorylation P53350 (PLK1) , Q13535 (ATR) , Q13315 (ATM) HPRD 10973490, 12493754, 12024051
S28 Phosphorylation Q13315 (ATM) , Q13535 (ATR) HPRD 10973490, 12024051
S33 Phosphorylation Q13315 (ATM) , Q13535 (ATR) HPRD 10973490
S35 Phosphorylation Q13315 (ATM) , Q13535 (ATR) HPRD 10973490
S50 Phosphorylation Q13535 (ATR) , Q13315 (ATM) HPRD 10973490
T68 Phosphorylation Q9H4B4 (PLK3) , P53350 (PLK1) , O96017 (CHEK2) , Q13315 (ATM) HPRD 16794575, 10973490, 11901158, ...12493754, 12242661
T383 Phosphorylation O96017 (CHEK2) HPRD 11390408
T387 Phosphorylation O96017 (CHEK2) HPRD 11390408
S516 Phosphorylation O96017 (CHEK2) HPRD 12805407, 11390408
Site PTM Type PTM Enzyme Score Source PMID
S19 Phosphorylation Q13535 (ATR) , Q13315 (ATM) HPRD 10973490
T26 Phosphorylation Q13315 (ATM) , P53350 (PLK1) , Q13535 (ATR) HPRD 10973490, 12493754, 12024051
S28 Phosphorylation Q13315 (ATM) , Q13535 (ATR) HPRD 10973490, 12024051
S33 Phosphorylation Q13315 (ATM) , Q13535 (ATR) HPRD 10973490
S35 Phosphorylation Q13535 (ATR) , Q13315 (ATM) HPRD 10973490
S50 Phosphorylation Q13315 (ATM) , Q13535 (ATR) HPRD 10973490
T68 Phosphorylation Q13315 (ATM) , O96017 (CHEK2) , P53350 (PLK1) , Q9H4B4 (PLK3) HPRD 16794575, 10973490, 11901158, ...12493754, 12242661
T354 Phosphorylation O96017 (CHEK2) HPRD 11390408
T358 Phosphorylation O96017 (CHEK2) HPRD 11390408
S487 Phosphorylation O96017 (CHEK2) HPRD 12805407, 11390408
Site PTM Type PTM Enzyme Score Source PMID
S19 Phosphorylation Q13315 (ATM) , Q13535 (ATR) HPRD 10973490
T26 Phosphorylation Q13315 (ATM) , P53350 (PLK1) , Q13535 (ATR) HPRD 10973490, 12493754, 12024051
S28 Phosphorylation Q13315 (ATM) , Q13535 (ATR) HPRD 10973490, 12024051
S33 Phosphorylation Q13315 (ATM) , Q13535 (ATR) HPRD 10973490
S35 Phosphorylation Q13315 (ATM) , Q13535 (ATR) HPRD 10973490
S50 Phosphorylation Q13315 (ATM) , Q13535 (ATR) HPRD 10973490
T68 Phosphorylation Q9H4B4 (PLK3) , P53350 (PLK1) , Q13315 (ATM) HPRD 16794575, 10973490, 11901158, ...12493754, 12242661
T426 Phosphorylation O96017 (CHEK2) HPRD 11390408
T430 Phosphorylation O96017 (CHEK2) HPRD 11390408
S559 Phosphorylation O96017 (CHEK2) HPRD 12805407, 11390408


O96017 (CHEK2) as PTM Enzyme



Substrate Site Score Source PMID
O00716 (E2F3) S124 PhosphoSitePlus 19917728
O15151 (MDM4) S342 HPRD   PhosphoSitePlus   Signor 16943424, 16163388
O15151 (MDM4) S367 HPRD   PhosphoSitePlus   Signor 16943424, 18356162, 16163388, ...16511560
O43255 (SIAH2) T26 PhosphoSitePlus 26751770
O43255 (SIAH2) S28 PhosphoSitePlus 26751770
O43255 (SIAH2) S68 PhosphoSitePlus 26751770
O43255 (SIAH2) T119 PhosphoSitePlus 26751770
O60673 (REV3L) S1075 PhosphoSitePlus 18622427
O96017 (CHEK2) S19 PhosphoSitePlus 18538787
O96017 (CHEK2) S33 PhosphoSitePlus 18538787
O96017 (CHEK2) S35 PhosphoSitePlus 18538787
O96017-1 (CHEK2) T68 HPRD   PhosphoSitePlus   Signor 12242661, 12493754, 16150728, ...12805407, 18644861, 12024051, 11901158, 10973490
O96017 (CHEK2) S120 PhosphoSitePlus 18538787
O96017 (CHEK2) S140 PhosphoSitePlus 18948271
O96017 (CHEK2) T225 PhosphoSitePlus 18538787
O96017 (CHEK2) S260 PhosphoSitePlus 18538787
O96017-12 (CHEK2) T354 HPRD 11390408
O96017-12 (CHEK2) T358 HPRD 11390408
O96017 (CHEK2) S379 neXtProt   PhosphoSitePlus   Signor   UniProt 18644861, 18538787
O96017 (CHEK2) T383 HPRD   PhosphoSitePlus   Signor   UniProt 12805407, 11390408, 11901158, ...18644861
O96017 (CHEK2) T387 HPRD   PhosphoSitePlus   Signor   UniProt 12805407, 11390408, 11901158, ...18644861
O96017-9 (CHEK2) T426 HPRD 11390408
O96017-9 (CHEK2) T430 HPRD 11390408
O96017 (CHEK2) T432 PhosphoSitePlus 18538787
O96017 (CHEK2) S435 PhosphoSitePlus 18538787
O96017-12 (CHEK2) S487 HPRD 12805407, 11390408
O96017 (CHEK2) S516 HPRD   PhosphoSitePlus   Signor 12805407, 12855706, 11390408, ...18644861, 18538787
O96017-9 (CHEK2) S559 HPRD 12805407, 11390408
P04637 (TP53) S15 PhosphoSitePlus   Signor 17339337, 10673501, 10710310, ...10656682
P04637 (TP53) T18 PhosphoSitePlus   Signor 17339337, 10673501, 10710310, ...10656682
P04637 (TP53) S20 PhosphoSitePlus   Signor 17339337, 10673500, 10801407, ...10710310, 10673501
P04637 (TP53) S37 PhosphoSitePlus   Signor 10673501, 10710310, 10656682, ...15659650
P04637 (TP53) S366 PhosphoSitePlus   Signor 10673501, 15659650
P04637 (TP53) S378 PhosphoSitePlus   Signor 17339337, 10710310, 15659650
P06400 (RB1) S612 neXtProt   PhosphoSitePlus   Signor 17380128
P10636-8 (MAPT) S262 PhosphoSitePlus 20159774
P10636 (MAPT) S579 Signor 21215781
P16403 (H1-2) S36 PhosphoSitePlus 17877366
P16403 (H1-2) S55 PhosphoSitePlus 17877366
P16403 (H1-2) S89 PhosphoSitePlus 17877366
P16403 (H1-2) T92 PhosphoSitePlus 17877366
P16403 (H1-2) T167 PhosphoSitePlus 17877366
P18887 (XRCC1) S184 PhosphoSitePlus 18971944
P18887 (XRCC1) S210 PhosphoSitePlus 18971944
P18887 (XRCC1) T284 PhosphoSitePlus   Signor 18971944
P21127 (CDK11B) S752 PhosphoSitePlus 23178491
P23560 (BDNF) T62 PhosphoSitePlus 23712400
P29590-3 (PML) S117 PhosphoSitePlus   Signor 12402044
P30304 (CDC25A) S124 PhosphoSitePlus   Signor 11298456, 12676583
P30304 (CDC25A) S178 HPRD   PhosphoSitePlus   Signor 12676583, 14559997
P30304-2 (CDC25A) S239 HPRD 12676583
P30304-2 (CDC25A) S253 HPRD 12676583
P30304 (CDC25A) S279 HPRD   PhosphoSitePlus   Signor 12676583
P30304 (CDC25A) S293 HPRD   PhosphoSitePlus   Signor 12676583
P30307 (CDC25C) S216 HPRD   neXtProt   PhosphoSitePlus   Signor 12835754, 10097108, 10744722, ...9543386, 16481012, 9836640, 10557092, 9278512, 16794575, 11333986, 11551930, 10786669, 20068231
P33981 (TTK) T288 PhosphoSitePlus   Signor 19151762, 24764296
P38398-8 (BRCA1) S941 HPRD 10724175
P38398 (BRCA1) S988 HPRD   PhosphoSitePlus   Signor 14701743, 10724175, 12111733
P40337 (VHL) S111 PhosphoSitePlus   Signor 22071692
P54132 (BLM) T182 PhosphoSitePlus 26028025
P61289 (PSME3) S247 PhosphoSitePlus 25361978
Q01094 (E2F1) S364 HPRD   PhosphoSitePlus   Signor 22558186, 12717439
Q08050-2 (FOXM1) S361 PhosphoSitePlus 17101782
Q08050 (FOXM1) S376 neXtProt   Signor 17101782
Q13263 (TRIM28) S473 PhosphoSitePlus 22715096, 22491012
Q13972 (RASGRF1) S287 Signor 17110335
Q15717 (ELAVL1) S88 PhosphoSitePlus 17317627, 21745814
Q15717 (ELAVL1) S100 PhosphoSitePlus 17317627, 21745814
Q15717 (ELAVL1) T118 PhosphoSitePlus 17317627, 21745814
Q9NRM7 (LATS2) S172 neXtProt   PhosphoSitePlus 21118956
Q9NRM7 (LATS2) S380 neXtProt   PhosphoSitePlus 21118956
Q9NRM7 (LATS2) S408 neXtProt   PhosphoSitePlus 21118956
Q9NRM7 (LATS2) S446 neXtProt   PhosphoSitePlus 21118956
Q9NY61 (AATF) S141 HPRD 17157788
Q9NY61 (AATF) S143 PhosphoSitePlus 17157788
Q9NY61 (AATF) S477 PhosphoSitePlus 17157788
Q9NY61 (AATF) S510 PhosphoSitePlus 17157788
Q9UQ88 (CDK11A) S740 neXtProt 23178491


Proteoforms

PRO ID (Short Label) Sites PTM Enzyme Source PMID
PR:000046702 (hCHEK2/Phos:2) pT68,pS379,pT383,pT387 PRO


PTM-dependent PPI

PTM type Substrate Site Interactant Association type Source PMID
Phosphorylation O96017 (CHEK2) T68 P28482 (MAPK1) unknown eFIP 21772273
Phosphorylation P04049 (RAF1) S338 O96017 (CHEK2) unknown eFIP 26333361


Proteoform PPIs

Protein 1 Relation Protein 2 Source PMID
PR:P29590-5 (hPML/iso:5) Interaction PR:O96017 (hCHEK2) PRO 12402044


PTM sites affected in variants

Site Variant Source PMID Disease [Sample source]
S19 * Biomuta DOID:2994 / germ cell cancer [ cosmic ]
S24 C24 Biomuta DOID:3571 / liver cancer [ icgc, tcga ] DOID:0070003 / blastoma [ cosmic ]
S42 C42 Biomuta DOID:4362 / cervical cancer [ tcga ]
T45 M45 Biomuta DOID:2394 / ovarian cancer [ tcga ] DOID:2994 / germ cell cancer [ cosmic ]
S52 P52 Biomuta DOID:1324 / lung cancer [ cosmic ]
S62 Y62 Biomuta DOID:3070 / malignant glioma [ tcga ]
S210 * Biomuta DOID:363 / uterine cancer [ cosmic, icgc, tcga ]
S210 L210 Biomuta DOID:1612 / breast cancer [ clinvar ]
K235 E235 Biomuta DOID:3070 / malignant glioma [ tcga ]
K235 Q235 Biomuta DOID:9256 / colorectal cancer [ icgc, tcga ] DOID:1909 / melanoma [ cosmic ]
K279 T279 Biomuta DOID:363 / uterine cancer [ cosmic, icgc, tcga ]
S357 F357 Biomuta DOID:1612 / breast cancer [ clinvar ]
S372 F372 Biomuta DOID:11934 / head and neck cancer [ tcga ]
K373 N373 Biomuta DOID:4362 / cervical cancer [ tcga ]
K373 E373 Biomuta DOID:1612 / breast cancer [ tcga ] DOID:3571 / liver cancer [ tcga ] DOID:1324 / lung cancer [ tcga ] DOID:263 / kidney cancer [ tcga ] DOID:2531 / hematologic cancer [ tcga ] DOID:169 / neuroendocrine tumor [ tcga ] DOID:363 / uterine cancer [ tcga ] DOID:2394 / ovarian cancer [ tcga ] DOID:5041 / esophageal cancer [ tcga ] DOID:2994 / germ cell cancer [ tcga ] DOID:1909 / melanoma [ tcga ] DOID:3070 / malignant glioma [ tcga ] DOID:1793 / pancreatic cancer [ tcga ] DOID:4362 / cervical cancer [ tcga ] DOID:10534 / stomach cancer [ tcga ] DOID:1115 / sarcoma [ tcga ] DOID:11934 / head and neck cancer [ tcga ] DOID:9256 / colorectal cancer [ tcga ] DOID:3953 / adrenal gland cancer [ tcga ] DOID:3963 / thyroid carcinoma [ icgc, tcga ] DOID:11054 / urinary bladder cancer [ tcga ]
T378 A378 Biomuta DOID:363 / uterine cancer [ tcga ]
S379 F379 Biomuta DOID:1612 / breast cancer [ tcga ]
S379 C379 Biomuta DOID:1612 / breast cancer [ cosmic ]
Y390 C390 Biomuta DOID:3070 / malignant glioma [ cosmic ]
Y390 * Biomuta DOID:3963 / thyroid carcinoma [ cosmic, icgc, tcga ]
Y390 N390 Biomuta DOID:11054 / urinary bladder cancer [ tcga ]