iPTMnet Report for O00418 (EEF2K)


Protein Information

Cytoscape View


UniProt AC / UniProt ID O00418 / EF2K_HUMAN
Protein Name Eukaryotic elongation factor 2 kinase
Gene Name Name: EEF2K
Organism Homo sapiens (Human)

PRO ID PR:O00418
DAG View
PRO Name eukaryotic elongation factor 2 kinase (human)
Definition An elongation factor 2 kinase that is encoded in the genome of human.
Short Label hEEF2K
Category organism-gene

Interactive Sequence View

Select/align proteoforms across species


O00418 (EEF2K) as Substrate



Site PTM Type PTM Enzyme Score Source PMID
A2 Acetylation UniProt 22223895
S18 Phosphorylation HPRD   phospho.ELM   PhosphoSitePlus   UniProt 19413330, 21406692, 23186163, ...16964243, 18767875, 19664994, 19664995, 15144186, 19651622, 17322306, 18669648, 19690332, 20068231, 17081983
S27 Phosphorylation HPRD   PhosphoSitePlus   UniProt 19413330, 17081983, 24275569
S31 Phosphorylation HPRD   PhosphoSitePlus 19413330, 17081983
Y37 Phosphorylation PhosphoSitePlus
K58 Ubiquitination PhosphoSitePlus
Y59 Phosphorylation PhosphoSitePlus
Y60 Phosphorylation PhosphoSitePlus
S61 Phosphorylation PhosphoSitePlus   RLIMS-P   UniProt 22216903
S61 Phosphorylation O00418 (EEF2K) UniProt 22216903
K65 Ubiquitination PhosphoSitePlus
S66 Phosphorylation PhosphoSitePlus   UniProt 22216903
S66 Phosphorylation O00418 (EEF2K) UniProt 22216903
Y69 Phosphorylation HPRD   PhosphoSitePlus 17081983
S70 Phosphorylation HPRD   PhosphoSitePlus   UniProt 23186163, 17081983
S71 Phosphorylation PhosphoSitePlus
S72 Phosphorylation HPRD   phospho.ELM   PhosphoSitePlus   UniProt 18669648, 23186163, 21659604
S72 Phosphorylation P42345 (MTOR) PhosphoSitePlus 21659604
S74 Phosphorylation HPRD   phospho.ELM   PhosphoSitePlus   UniProt 18669648, 23186163, 21659604
S74 Phosphorylation P42345 (MTOR) PhosphoSitePlus 21659604
S78 Phosphorylation HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   UniProt 22669845, 23186163, 32054489, ...14709557, 22216903, 14684180, 21112387, 22329831, 15024086
S78 Phosphorylation O00418 (EEF2K) neXtProt   UniProt 22669845, 21112387, 22329831, ...22216903
S78 Phosphorylation P54646 (PRKAA2) HPRD 14709557, 15024086
S78 Phosphorylation Q13131 (PRKAA1) HPRD 14709557, 15024086
S78 Phosphorylation Q96QT4 (TRPM7) neXtProt   PhosphoSitePlus 21112387
K82 Ubiquitination PhosphoSitePlus
K86 Ubiquitination PhosphoSitePlus
K93 Ubiquitination PhosphoSitePlus
K132 Ubiquitination PhosphoSitePlus
S135 Phosphorylation HPRD   phospho.ELM   PhosphoSitePlus 17287340
C146 Methylation IEDB 31844290
K151 Ubiquitination PhosphoSitePlus
K162 Ubiquitination PhosphoSitePlus
K170 Ubiquitination PhosphoSitePlus
R178 Methylation PhosphoSitePlus
K202 Ubiquitination PhosphoSitePlus
K223 Ubiquitination PhosphoSitePlus
K235 Ubiquitination PhosphoSitePlus
K238 Ubiquitination PhosphoSitePlus
S241 Phosphorylation PhosphoSitePlus
S243 Phosphorylation PhosphoSitePlus   UniProt 23186163
T340 Phosphorylation PhosphoSitePlus
K341 Ubiquitination PhosphoSitePlus
S345 Phosphorylation PhosphoSitePlus   RLIMS-P 22216903
K347 Ubiquitination PhosphoSitePlus
T348 Phosphorylation HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   UniProt 22669845, 22329831, 24275569, ...27956550, 25670349, 18669648, 22115317, 22216903, 23186163, 20068231
T348 Phosphorylation O00418 (EEF2K) neXtProt   PhosphoSitePlus   UniProt 22669845, 22329831, 18669648, ...22216903
T353 Phosphorylation neXtProt   PhosphoSitePlus   RLIMS-P   UniProt 22669845, 22329831, 22115317, ...22216903, 23186163
T353 Phosphorylation O00418 (EEF2K) neXtProt   PhosphoSitePlus   UniProt 22669845, 22329831, 22216903
S359 Phosphorylation HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 18337751, 11500363, 12171600, ...22216903
S359 Phosphorylation O15264 (MAPK13) HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   Signor 12171600, 18337751, 11500363
S359 Phosphorylation P06493 (CDK1) neXtProt   PhosphoSitePlus   Signor 18337751
T364 Phosphorylation PhosphoSitePlus
S366 Phosphorylation HPRD   IEDB   neXtProt   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P   Signor   UniProt 32897882, 22669845, 22329831, ...20837011, 32054489, 14709557, 22216903, 11500364, 15024086, 11171059
S366 Phosphorylation O00418 (EEF2K) neXtProt   PhosphoSitePlus   UniProt 11500364, 22669845, 22329831, ...22216903
S366 Phosphorylation P17612 (PRKACA) HPRD   neXtProt 11500364, 14709557, 15024086, ...11171059
S366 Phosphorylation P23443 (RPS6KB1) HPRD   neXtProt   PhosphoSitePlus   Signor 14709557, 11500364, 15024086, ...11171059
S366 Phosphorylation P54646 (PRKAA2) HPRD 11500364, 14709557, 15024086, ...11171059
S366 Phosphorylation Q13131 (PRKAA1) HPRD 11500364, 14709557, 15024086, ...11171059
S366 Phosphorylation Q15418 (RPS6KA1) HPRD   neXtProt   PhosphoSitePlus   Signor 11500364, 20837011, 14709557, ...15024086, 11171059
S366 Phosphorylation Q9UBS0 (RPS6KB2) neXtProt   PhosphoSitePlus 11500364
S377 Phosphorylation HPRD   neXtProt   phospho.ELM   PhosphoSitePlus 12171600
S377 Phosphorylation P49137 (MAPKAPK2) HPRD   neXtProt   phospho.ELM   PhosphoSitePlus 12171600
S377 Phosphorylation Q16644 (MAPKAPK3) HPRD   neXtProt 12171600
S377 Phosphorylation Q8IW41 (MAPKAPK5) HPRD   neXtProt 12171600
S380 Phosphorylation PhosphoSitePlus
S392 Phosphorylation PhosphoSitePlus
S395 Phosphorylation PhosphoSitePlus
S396 Phosphorylation HPRD   neXtProt   phospho.ELM   PhosphoSitePlus 12171600, 11500363, 14709557
S396 Phosphorylation O00418 (EEF2K) PhosphoSitePlus 14709557
S396 Phosphorylation O15264 (MAPK13) HPRD   neXtProt   PhosphoSitePlus 12171600, 11500363
S396 Phosphorylation P45983 (MAPK8) HPRD 12171600
S396 Phosphorylation P45984 (MAPK9) HPRD 12171600
S396 Phosphorylation P53778 (MAPK12) HPRD 12171600
S396 Phosphorylation Q15759 (MAPK11) HPRD   neXtProt   PhosphoSitePlus 12171600
S396 Phosphorylation Q16539 (MAPK14) HPRD   neXtProt 12171600
S398 Phosphorylation HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 20472894, 22669845, 22749997, ...23182879, 14709557, 15024086
S398 Phosphorylation P54646 (PRKAA2) HPRD   PhosphoSitePlus 23182879, 14709557, 15024086
S398 Phosphorylation Q13131 (PRKAA1) HPRD   neXtProt   PhosphoSitePlus   Signor 22669845, 14709557, 15024086
S399 Phosphorylation PhosphoSitePlus
T401 Phosphorylation PhosphoSitePlus
S416 Phosphorylation PhosphoSitePlus
S435 Phosphorylation PhosphoSitePlus   UniProt 23186163
S438 Phosphorylation PhosphoSitePlus
S441 Phosphorylation neXtProt   PhosphoSitePlus 22669845, 22329831, 22216903
S441 Phosphorylation O00418 (EEF2K) neXtProt   PhosphoSitePlus 22669845, 22329831, 22216903
Y443 Phosphorylation PhosphoSitePlus
S445 Phosphorylation HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 22669845, 23186163, 16964243, ...19651622, 24275569, 22216903, 22329831, 20068231
S445 Phosphorylation O00418 (EEF2K) neXtProt   PhosphoSitePlus   Signor   UniProt 22669845, 22329831, 16964243, ...22216903
K447 Ubiquitination PhosphoSitePlus
S462 Phosphorylation PhosphoSitePlus
K467 Sumoylation PhosphoSitePlus
Y468 Phosphorylation HPRD   phospho.ELM 18669648
S470 Phosphorylation HPRD   phospho.ELM   PhosphoSitePlus   UniProt 19413330, 21406692, 23186163, ...17081983, 18767875, 17322306, 18669648, 20068231, 19415658, 19651622
S474 Phosphorylation HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   UniProt 19413330, 22669845, 23186163, ...17081983, 19651622, 18669648, 24275569, 22216903, 22329831, 19415658, 20068231
S474 Phosphorylation O00418 (EEF2K) neXtProt   PhosphoSitePlus   UniProt 22669845, 22329831, 17081983, ...22216903
S477 Phosphorylation HPRD   phospho.ELM   PhosphoSitePlus   RLIMS-P   UniProt 18669648, 23186163, 22216903
S478 Phosphorylation PhosphoSitePlus
K485 Ubiquitination PhosphoSitePlus
S491 Phosphorylation neXtProt   PhosphoSitePlus   RLIMS-P   UniProt 22329831, 22669845, 23186163, ...22216903
S491 Phosphorylation O00418 (EEF2K) neXtProt   UniProt 22669845, 22329831, 22216903
S492 Phosphorylation PhosphoSitePlus
S500 Phosphorylation HPRD   neXtProt   PhosphoSitePlus   RLIMS-P 22669845, 22329831, 27956550, ...22216903, 11171059
S500 Phosphorylation O00418 (EEF2K) neXtProt   PhosphoSitePlus 22669845, 22329831, 22216903
S500 Phosphorylation P17612 (PRKACA) HPRD   neXtProt 11171059
K516 Ubiquitination PhosphoSitePlus
K517 Ubiquitination PhosphoSitePlus
K520 Ubiquitination PhosphoSitePlus
S521 Phosphorylation PhosphoSitePlus
K596 Ubiquitination PhosphoSitePlus
Y600 Phosphorylation HPRD   PhosphoSitePlus 20068231
K603 Ubiquitination PhosphoSitePlus
S627 Phosphorylation PhosphoSitePlus   RLIMS-P 22216903
K684 Ubiquitination PhosphoSitePlus
K705 Ubiquitination PhosphoSitePlus
K715 Ubiquitination PhosphoSitePlus


O00418 (EEF2K) as PTM Enzyme



Substrate Site Score Source PMID
O00418 (EEF2K) S61 UniProt 22216903
O00418 (EEF2K) S66 UniProt 22216903
O00418 (EEF2K) S78 neXtProt   UniProt 22669845, 21112387, 22329831, ...22216903
O00418 (EEF2K) T348 neXtProt   PhosphoSitePlus   UniProt 22669845, 22329831, 18669648, ...22216903
O00418 (EEF2K) T353 neXtProt   PhosphoSitePlus   UniProt 22669845, 22329831, 22216903
O00418 (EEF2K) S366 neXtProt   PhosphoSitePlus   UniProt 11500364, 22669845, 22329831, ...22216903
O00418 (EEF2K) S396 PhosphoSitePlus 14709557
O00418 (EEF2K) S441 neXtProt   PhosphoSitePlus 22669845, 22329831, 22216903
O00418 (EEF2K) S445 neXtProt   PhosphoSitePlus   Signor   UniProt 22669845, 22329831, 16964243, ...22216903
O00418 (EEF2K) S474 neXtProt   PhosphoSitePlus   UniProt 22669845, 22329831, 17081983, ...22216903
O00418 (EEF2K) S491 neXtProt   UniProt 22669845, 22329831, 22216903
O00418 (EEF2K) S500 neXtProt   PhosphoSitePlus 22669845, 22329831, 22216903
P13639 (EEF2) T54 HPRD 91085562, 20068231
P13639 (EEF2) T57 HPRD   neXtProt   Signor 17287340, 18212344, 12171600, ...9133370, 19651622, 18669648, 8386634, 20068231, 18767875, 20166139, 18614030, 11500363, 12194824, 18188169
P13639 (EEF2) T59 HPRD   Signor 19413330, 2261989, 18767875, ...19651622, 18669648, 8386634, 20068231


Proteoforms

PRO ID (Short Label) Sites PTM Enzyme Source PMID
PR:000047251 (hEEF2K/Phos:2) pS366 PRO


PTM-dependent PPI

PTM type Substrate Site Interactant Association type Source PMID
Phosphorylation O00418 (EEF2K) S78 P0DP23 (CALM1) unknown eFIP 14684180


PTM sites affected in variants

Site Variant Source PMID Disease [Sample source]
S18 F18 Biomuta DOID:4159 / skin cancer [ cosmic ]
S31 N31 Biomuta DOID:4362 / cervical cancer [ cosmic, icgc ]
S61 I61 Biomuta DOID:363 / uterine cancer [ icgc ]
S61 G61 Biomuta DOID:4362 / cervical cancer [ icgc ]
Y69 H69 Biomuta DOID:2531 / hematologic cancer [ cosmic ]
S71 F71 Biomuta DOID:10534 / stomach cancer [ cosmic ]
S74 C74 Biomuta DOID:1324 / lung cancer [ cosmic, icgc ]
K132 Q132 Biomuta DOID:1612 / breast cancer [ cosmic, icgc ]
S135 C135 Biomuta DOID:5041 / esophageal cancer [ cosmic ] DOID:1324 / lung cancer [ cosmic ]
C146 R146 Biomuta DOID:9256 / colorectal cancer [ cosmic, icgc ]
R178 W178 Biomuta DOID:4159 / skin cancer [ cosmic ] DOID:9256 / colorectal cancer [ cosmic, icgc ]
S243 Y243 Biomuta DOID:1781 / thyroid gland cancer [ cosmic ] DOID:1781 / thyroid cancer [ icgc ]
S243 P243 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
S243 F243 Biomuta DOID:5041 / esophageal cancer [ cosmic ]
K347 N347 Biomuta DOID:363 / uterine cancer [ cosmic, icgc ] DOID:3571 / liver cancer [ cosmic ]
T364 N364 Biomuta DOID:263 / kidney cancer [ cosmic ]
S366 F366 Biomuta DOID:5041 / esophageal cancer [ cosmic ] DOID:11054 / urinary bladder cancer [ cosmic, icgc ]
S366 C366 Biomuta DOID:1324 / lung cancer [ cosmic, icgc ]
S377 L377 Biomuta DOID:4159 / skin cancer [ cosmic ]
S392 F392 Biomuta DOID:4159 / skin cancer [ cosmic, icgc ]
S396 F396 Biomuta DOID:4159 / skin cancer [ cosmic, icgc ]
S398 C398 Biomuta DOID:1612 / breast cancer [ cosmic ]
S399 L399 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
S416 G416 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
S462 L462 Biomuta DOID:1612 / breast cancer [ cosmic ]
Y468 * Biomuta DOID:8618 / oral cavity cancer [ cosmic ]
S478 F478 Biomuta DOID:4159 / skin cancer [ cosmic ]
S500 L500 Biomuta DOID:9256 / colorectal cancer [ cosmic ]