Protein Information

Cytoscape View


UniProt AC / UniProt ID Q9Y6D9 / MD1L1_HUMAN
Protein Name Mitotic spindle assembly checkpoint protein MAD1
Gene Name Name: MAD1L1
Synonyms:MAD1,TXBP181;
Organism Homo sapiens (Human)

PRO ID PR:Q9Y6D9
DAG View
PRO Name mitotic spindle assembly checkpoint protein MAD1 (human)
Definition A mitotic spindle assembly checkpoint protein MAD1 that is encoded in the genome of human.
Short Label hMAD1L1
Category organism-gene

Interactive Sequence View

Select/align proteoforms across species


Q9Y6D9 (MAD1L1) as Substrate



Site PTM Type PTM Enzyme Score Source PMID
M1 Acetylation UniProt 22814378, 22223895
T8 Phosphorylation PhosphoSitePlus
S12 Phosphorylation phospho.ELM   PhosphoSitePlus 22067460
T13 Phosphorylation phospho.ELM   PhosphoSitePlus 22067460
S16 Phosphorylation phospho.ELM   PhosphoSitePlus   UniProt 22067460, 18669648, 23186163
S22 Phosphorylation P53350 (PLK1) PhosphoSitePlus 18922800
S29 Phosphorylation P53350 (PLK1) PhosphoSitePlus 18922800
K61 Acetylation PhosphoSitePlus   UniProt 19608861
K61 Sumoylation PhosphoSitePlus
K61 Ubiquitination PhosphoSitePlus
S62 Phosphorylation PhosphoSitePlus
S77 Phosphorylation phospho.ELM   PhosphoSitePlus 22067460
K79 Ubiquitination PhosphoSitePlus
S90 Phosphorylation phospho.ELM   PhosphoSitePlus 22067460
T91 Phosphorylation PhosphoSitePlus
S92 Phosphorylation phospho.ELM   PhosphoSitePlus 22067460
Y96 Phosphorylation PhosphoSitePlus
K123 Ubiquitination PhosphoSitePlus
K143 Acetylation PhosphoSitePlus
K143 Ubiquitination PhosphoSitePlus
K148 Ubiquitination PhosphoSitePlus
K163 Ubiquitination PhosphoSitePlus
S185 Phosphorylation PhosphoSitePlus
K207 Ubiquitination PhosphoSitePlus
S214 Phosphorylation Q13315 (ATM) phospho.ELM   PhosphoSitePlus   RLIMS-P   UniProt 17525332, 24728176, 32944396
K231 Ubiquitination PhosphoSitePlus
S233 Phosphorylation PhosphoSitePlus
K313 Ubiquitination PhosphoSitePlus
T323 Phosphorylation PhosphoSitePlus
K351 Ubiquitination PhosphoSitePlus
S377 Phosphorylation PhosphoSitePlus
K406 Ubiquitination PhosphoSitePlus
T423 Phosphorylation PhosphoSitePlus
Y427 Phosphorylation phospho.ELM 18669648
S428 Phosphorylation phospho.ELM   PhosphoSitePlus   UniProt 23186163, 16964243, 18220336, ...15302935, 16565220, 22067460, 18669648, 20068231
T432 Phosphorylation PhosphoSitePlus
K467 Ubiquitination PhosphoSitePlus
K478 Acetylation PhosphoSitePlus
K481 Ubiquitination PhosphoSitePlus
S484 Phosphorylation PhosphoSitePlus
S485 Phosphorylation PhosphoSitePlus
S486 Phosphorylation PhosphoSitePlus
S490 Phosphorylation phospho.ELM   PhosphoSitePlus 22067460
S494 Phosphorylation PhosphoSitePlus
T500 Phosphorylation PhosphoSitePlus
K504 Ubiquitination PhosphoSitePlus
S513 Phosphorylation PhosphoSitePlus
Y535 Phosphorylation PhosphoSitePlus
S538 Phosphorylation PhosphoSitePlus
S546 Phosphorylation PhosphoSitePlus
S551 Phosphorylation phospho.ELM   PhosphoSitePlus 22067460
S562 Phosphorylation PhosphoSitePlus
T583 Phosphorylation PhosphoSitePlus
S597 Phosphorylation PhosphoSitePlus
S598 Phosphorylation PhosphoSitePlus   UniProt 29162720
K599 Ubiquitination PhosphoSitePlus
S610 Phosphorylation PhosphoSitePlus   UniProt 29162720
K614 Ubiquitination PhosphoSitePlus
K619 Ubiquitination PhosphoSitePlus
T624 Phosphorylation PhosphoSitePlus
K625 Ubiquitination PhosphoSitePlus
Y634 Phosphorylation PhosphoSitePlus   UniProt 29162720
T644 Phosphorylation RLIMS-P 28072388
T680 Phosphorylation P53350 (PLK1) PhosphoSitePlus   RLIMS-P 18922800
S699 Phosphorylation PhosphoSitePlus
T708 Phosphorylation PhosphoSitePlus
T716 Phosphorylation PhosphoSitePlus   RLIMS-P   UniProt 28072388, 29162720, 32202322
Site PTM Type PTM Enzyme Score Source PMID
S12 Phosphorylation HPRD 20068231
S16 Phosphorylation HPRD 18669648, 20068231
S214 Phosphorylation HPRD 17525332
T323 Phosphorylation HPRD 20068231
Y427 Phosphorylation HPRD 18669648, 20068231, 16565220
S428 Phosphorylation HPRD 19413330, 18691976, 16964243, ...19664995, 18452278, 19651622, 18669648, 20068231
S485 Phosphorylation HPRD 20068231
S490 Phosphorylation HPRD 19691289
T550 Phosphorylation HPRD 20068231
S594 Phosphorylation HPRD 20068231


PTM-dependent PPI

PTM type Substrate Site Interactant Association type Source PMID
Phosphorylation Q9Y6D9 (MAD1L1) S214 P13010 (XRCC5) unknown eFIP 32944396
Phosphorylation Q9Y6D9 (MAD1L1) T716 Q12834 (CDC20) increased_association eFIP 28072388
Phosphorylation Q9Y6D9 (MAD1L1) T716 Q12834 (CDC20) increased_association eFIP 29868582


Proteoform PPIs

Protein 1 Relation Protein 2 Source PMID
PR:O43683 (hBUB1) Interaction PR:Q9Y6D9 (hMAD1L1) PRO 10198256
PR:O43684 (hBUB3) Interaction PR:Q9Y6D9 (hMAD1L1) PRO 10198256
PR:Q13257 (hMAD2L1) Interaction PR:Q9Y6D9 (hMAD1L1) PRO
PR:Q9Y6D9 (hMAD1L1) Interaction PR:Q13257 (hMAD2L1) PRO 10198256
PR:Q9Y6D9 (hMAD1L1) Interaction PR:O43684 (hBUB3) PRO 10198256
PR:Q9Y6D9 (hMAD1L1) Interaction PR:O43683 (hBUB1) PRO 10198256


PTM sites affected in variants

Site Variant Source PMID Disease [Sample source]
S16 F16 Biomuta DOID:3070 / malignant glioma [ cosmic, icgc, tcga ]
S77 N77 Biomuta DOID:3571 / liver cancer [ icgc ] DOID:0070003 / blastoma [ cosmic ]
K79 R79 Biomuta DOID:363 / uterine cancer [ tcga ]
S185 * Biomuta DOID:9256 / colorectal cancer [ tcga ]
T432 M432 Biomuta DOID:2531 / hematologic cancer [ icgc ]
K478 N478 Biomuta DOID:363 / uterine cancer [ cosmic, icgc, tcga ]
S485 C485 Biomuta DOID:3571 / liver cancer [ icgc ] DOID:0070003 / blastoma [ cosmic ]
S490 G490 Biomuta DOID:363 / uterine cancer [ cosmic, icgc, tcga ]
K614 N614 Biomuta DOID:1612 / breast cancer [ tcga ]
T708 I708 Biomuta DOID:263 / kidney cancer [ cosmic, icgc, tcga ]