iPTMnet Report for Q13485 (SMAD4)


Protein Information

Cytoscape View


UniProt AC / UniProt ID Q13485 / SMAD4_HUMAN
Protein Name Mothers against decapentaplegic homolog 4
Gene Name Name: SMAD4
Synonyms:DPC4,MADH4;
Organism Homo sapiens (Human)

PRO ID PR:Q13485
DAG View
PRO Name mothers against decapentaplegic homolog 4 (human)
Definition A smad4 that is encoded in the genome of human.
Short Label hSMAD4
Category organism-gene

Interactive Sequence View

Select/align proteoforms across species


Q13485 (SMAD4) as Substrate



Site PTM Type PTM Enzyme Score Source PMID
T9 Phosphorylation Q9UBE8 (NLK) neXtProt   PhosphoSitePlus   RLIMS-P 19690946
S22 Phosphorylation PhosphoSitePlus
S32 Phosphorylation PhosphoSitePlus
K37 Acetylation PhosphoSitePlus   UniProt 19608861
K45 Acetylation PhosphoSitePlus
K45 Ubiquitination PhosphoSitePlus
K48 Acetylation PhosphoSitePlus
T59 O-Glycosylation GlyGen 33199824, 33214551, 34019948, ...29351928
T62 O-Glycosylation GlyGen 33199824, 33214551, 34019948, ...29351928
T63 O-Glycosylation GlyGen 33199824, 33214551, 34019948, ...29351928
S69 O-Glycosylation GlyGen 33199824, 33214551, 34019948, ...29351928
K70 Ubiquitination PhosphoSitePlus
T77 Phosphorylation Q15831 (STK11) neXtProt   PhosphoSitePlus   RLIMS-P 20974850
Y95 Phosphorylation PhosphoSitePlus   RLIMS-P 30664791
K106 Acetylation PhosphoSitePlus
K113 Sumoylation PhosphoSitePlus
K113 Ubiquitination PhosphoSitePlus
K122 Ubiquitination PhosphoSitePlus
C123 Methylation IEDB 31844290
C127 Methylation IEDB 31844290
S138 Phosphorylation Q9UBE8 (NLK) neXtProt   PhosphoSitePlus   RLIMS-P 19690946
K159 Sumoylation PhosphoSitePlus
S178 Phosphorylation PhosphoSitePlus
Y195 Phosphorylation PhosphoSitePlus
T197 Phosphorylation PhosphoSitePlus
S206 O-Glycosylation GlyGen 33199824, 33214551, 34019948, ...29351928
T209 O-Glycosylation GlyGen 33199824, 33214551, 34019948, ...29351928
T211 O-Glycosylation GlyGen 33199824, 33214551, 34019948, ...29351928
S221 O-Glycosylation GlyGen 33199824, 33214551, 34019948, ...29351928
S223 O-Glycosylation GlyGen 33199824, 33214551, 34019948, ...29351928
T265 Phosphorylation PhosphoSitePlus   RLIMS-P 33199824
T269 Phosphorylation PhosphoSitePlus   RLIMS-P 33199824
T273 Phosphorylation PhosphoSitePlus   RLIMS-P 33199824
T277 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3) HPRD   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor 33199824, 27308538, 28115363, ...12801888
Y301 Phosphorylation PhosphoSitePlus
Y322 Phosphorylation PhosphoSitePlus
S343 Phosphorylation Q14680 (MELK) neXtProt   PhosphoSitePlus 20659902
Y353 Phosphorylation RLIMS-P 31681835
K385 Ubiquitination PhosphoSitePlus
C401 Methylation IEDB 31844290
Y412 Phosphorylation RLIMS-P 31681835
K428 Acetylation PhosphoSitePlus   UniProt 19608861
S504 Phosphorylation PhosphoSitePlus
K507 Acetylation PhosphoSitePlus   UniProt 19608861
K507 Ubiquitination PhosphoSitePlus
Y513 Phosphorylation PhosphoSitePlus
K519 Ubiquitination PhosphoSitePlus   UniProt 19135894
Site PTM Type PTM Enzyme Score Source PMID
T277 Phosphorylation PRO 12801888
K507 Ubiquitination PRO 18783722
K519 Ubiquitination PRO 19135894


Proteoforms

PRO ID (Short Label) Sites PTM Enzyme Source PMID
PR:000025956 (hSMAD4/iso:1/MonoUbiq:1) ubK507 PRO 18783722
PR:000025958 (hSMAD4/iso:1/MonoUbiq:2) ubK519 PRO 19135894
PR:000036483 (hSMAD4/iso:1/Phos:1) pT277 PRO 12801888


PTM-dependent PPI

PTM type Substrate Site Interactant Association type Source PMID
Phosphorylation P84022 (SMAD3) T388 Q13485 (SMAD4) increased_association eFIP 24485922
Phosphorylation Q15796 (SMAD2) S465 Q13485 (SMAD4) unknown eFIP 9346908


Proteoform PPIs

Protein 1 Relation Protein 2 Source PMID
PR:000025934 (hSMAD2/iso:Long/Phos:1) Interaction PR:Q13485 (hSMAD4) PRO 9311995
PR:000025935 (hSMAD2/iso:Long/Phos:2) Interaction PR:Q13485 (hSMAD4) PRO 12193595
PR:000025936 (hSMAD2/iso:Long/Phos:3) Interaction PR:Q13485 (hSMAD4) PRO 11027280
PR:000026333 (hSMAD3/iso:1/Phos:1) Interaction PR:Q13485 (hSMAD4) PRO 15350224, 9311995
PR:000026334 (hSMAD1/iso:1/Phos:1) Interaction PR:Q13485 (hSMAD4) PRO 9136927


PTM sites affected in variants

Site Variant Source PMID Disease [Sample source]
S32 T32 Biomuta DOID:4362 / cervical cancer [ icgc ]
K45 N45 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
K70 T70 Biomuta DOID:363 / uterine cancer [ cosmic, icgc ]
K70 * Biomuta DOID:1793 / pancreatic cancer [ cosmic ]
T77 A77 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
Y95 * Biomuta DOID:1793 / pancreatic cancer [ cosmic ]
Y95 F95 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
Y95 S95 Biomuta DOID:1793 / pancreatic cancer [ cosmic ]
Y95 H95 Biomuta DOID:9256 / colorectal cancer [ cosmic ] DOID:4606 / bile duct cancer [ cosmic ] DOID:1324 / lung cancer [ cosmic ]
Y95 N95 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
Y95 C95 Biomuta DOID:1793 / pancreatic cancer [ cosmic ] DOID:1324 / lung cancer [ cosmic, icgc ] DOID:263 / kidney cancer [ cosmic, icgc ]
K106 R106 Biomuta DOID:1324 / lung cancer [ cosmic ]
K122 * Biomuta DOID:4159 / skin cancer [ cosmic ] DOID:10534 / stomach cancer [ cosmic ]
C123 R123 Biomuta DOID:4159 / skin cancer [ cosmic ]
C127 S127 Biomuta DOID:1793 / pancreatic cancer [ cosmic ]
C127 F127 Biomuta DOID:1793 / pancreatic cancer [ cosmic ] DOID:9256 / colorectal cancer [ cosmic ] DOID:9253 / gastrointestinal stromal tumor [ cosmic ] DOID:1324 / lung cancer [ cosmic ]
C127 G127 Biomuta DOID:4606 / bile duct cancer [ cosmic ]
C127 * Biomuta DOID:1324 / lung cancer [ cosmic ]
S138 L138 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
S138 * Biomuta DOID:1793 / pancreatic cancer [ cosmic ] DOID:9256 / colorectal cancer [ cosmic ] DOID:1324 / lung cancer [ cosmic ]
K159 N159 Biomuta DOID:1793 / pancreatic cancer [ cosmic ]
S178 * Biomuta DOID:4606 / bile duct cancer [ cosmic ] DOID:4362 / cervical cancer [ cosmic ] DOID:1793 / pancreatic cancer [ cosmic ] DOID:10534 / stomach cancer [ cosmic ] DOID:9256 / colorectal cancer [ cosmic ] DOID:11054 / urinary bladder cancer [ cosmic ]
Y195 * Biomuta DOID:8618 / oral cavity cancer [ cosmic ] DOID:9256 / colorectal cancer [ cosmic ]
T197 I197 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
T197 A197 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
S221 P221 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
T265 I265 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
T273 S273 Biomuta DOID:11054 / urinary bladder cancer [ cosmic ]
Y322 * Biomuta DOID:1793 / pancreatic cancer [ cosmic ]
S343 * Biomuta DOID:1793 / pancreatic cancer [ cosmic ] DOID:1612 / breast cancer [ cosmic ] DOID:1324 / lung cancer [ cosmic ] DOID:9256 / colorectal cancer [ cosmic ] DOID:4606 / bile duct cancer [ cosmic ]
S343 L343 Biomuta DOID:9256 / colorectal cancer [ cosmic ] DOID:11054 / urinary bladder cancer [ cosmic ]
Y353 * Biomuta DOID:10534 / stomach cancer [ cosmic ]
Y353 C353 Biomuta DOID:9256 / colorectal cancer [ cosmic ] DOID:1324 / lung cancer [ cosmic ] DOID:4362 / cervical cancer [ cosmic, icgc ] DOID:5041 / esophageal cancer [ cosmic ] DOID:1793 / pancreatic cancer [ cosmic ] DOID:10534 / stomach cancer [ cosmic ] DOID:9253 / gastrointestinal stromal tumor [ cosmic ]
Y353 D353 Biomuta DOID:10534 / stomach cancer [ cosmic ]
Y353 H353 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
Y353 N353 Biomuta DOID:9256 / colorectal cancer [ cosmic ] DOID:10283 / prostate cancer [ cosmic, icgc ] DOID:10534 / stomach cancer [ cosmic ] DOID:4606 / bile duct cancer [ cosmic ]
K385 * Biomuta DOID:9256 / colorectal cancer [ cosmic ]
C401 R401 Biomuta DOID:3571 / liver cancer [ cosmic ]
C401 F401 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
Y412 * Biomuta DOID:1793 / pancreatic cancer [ cosmic ]
Y412 D412 Biomuta DOID:11054 / urinary bladder cancer [ cosmic, icgc ]
K428 M428 Biomuta DOID:9256 / colorectal cancer [ cosmic, icgc ] DOID:1324 / lung cancer [ cosmic ]
K428 R428 Biomuta DOID:4606 / bile duct cancer [ cosmic ]
K428 T428 Biomuta DOID:363 / uterine cancer [ cosmic, icgc ] DOID:9256 / colorectal cancer [ cosmic ]
K428 N428 Biomuta DOID:1793 / pancreatic cancer [ cosmic ] DOID:1612 / breast cancer [ cosmic ] DOID:3571 / liver cancer [ cosmic ] DOID:1324 / lung cancer [ cosmic, icgc ] DOID:9256 / colorectal cancer [ cosmic ]
S504 R504 Biomuta [ cosmic ] DOID:10283 / prostate cancer [ cosmic, icgc ] DOID:2394 / ovarian cancer [ cosmic ] DOID:1793 / pancreatic cancer [ cosmic ] DOID:10534 / stomach cancer [ cosmic, icgc ] DOID:9256 / colorectal cancer [ cosmic ] DOID:3119 / gastrointestinal system cancer [ cosmic ]
K507 E507 Biomuta DOID:9256 / colorectal cancer [ cosmic ] DOID:10534 / stomach cancer [ cosmic ]
K507 N507 Biomuta DOID:9256 / colorectal cancer [ cosmic ] DOID:10534 / stomach cancer [ cosmic, icgc ] DOID:1324 / lung cancer [ cosmic ]
K507 Q507 Biomuta DOID:9256 / colorectal cancer [ cosmic ] DOID:4606 / bile duct cancer [ cosmic ]
Y513 * Biomuta DOID:9256 / colorectal cancer [ cosmic ]
Y513 H513 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
K519 * Biomuta DOID:1324 / lung cancer [ cosmic ]
K519 N519 Biomuta DOID:363 / uterine cancer [ cosmic, icgc ]
K519 E519 Biomuta DOID:9256 / colorectal cancer [ cosmic ] DOID:10534 / stomach cancer [ cosmic ]