iPTMnet Report for Q02750 (MAP2K1)


Protein Information

Cytoscape View


UniProt AC / UniProt ID Q02750 / MP2K1_HUMAN
Protein Name Dual specificity mitogen-activated protein kinase kinase 1
Gene Name Name: MAP2K1
Synonyms:MEK1,PRKMK1;
Organism Homo sapiens (Human)

PRO ID PR:Q02750
DAG View
PRO Name dual specificity mitogen-activated protein kinase kinase 1 (human)
Definition A dual specificity mitogen-activated protein kinase kinase 1 that is encoded in the genome of human.
Short Label hMAP2K1
Category organism-gene

Interactive Sequence View

Select/align proteoforms across species


Q02750 (MAP2K1) as Substrate



Site PTM Type PTM Enzyme Score Source PMID
T23 Phosphorylation phospho.ELM 7873542
S24 Phosphorylation neXtProt   phospho.ELM 21441910, 7873542
S24 Phosphorylation Q6VAB6 (KSR2) neXtProt 21441910
S25 Phosphorylation phospho.ELM 7873542
K36 Ubiquitination PhosphoSitePlus
T55 Phosphorylation PhosphoSitePlus 21851590
T55 Phosphorylation O14757 (CHEK1) PhosphoSitePlus 21851590
K57 Ubiquitination PhosphoSitePlus
K64 Ubiquitination PhosphoSitePlus
K70 Ubiquitination PhosphoSitePlus
S72 Phosphorylation neXtProt   PhosphoSitePlus 21441910
S72 Phosphorylation Q6VAB6 (KSR2) neXtProt 21441910
K88 Ubiquitination PhosphoSitePlus
K97 Ubiquitination PhosphoSitePlus
K104 Sumoylation PhosphoSitePlus
K104 Ubiquitination PhosphoSitePlus
K168 Ubiquitination PhosphoSitePlus
K175 Acetylation PhosphoSitePlus
K175 Ubiquitination PhosphoSitePlus
K192 Ubiquitination PhosphoSitePlus
S194 Phosphorylation PhosphoSitePlus
K205 Ubiquitination PhosphoSitePlus
S212 Phosphorylation neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P 12506122, 22177953
S218 Phosphorylation neXtProt   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P   Signor   UniProt 27169363, 10359597, 29753091, ...10037701, 8952507, 17667937, 8034665, 29433126, 33808483, 20956560, 8131746, 15466476, 11042690, 14993270, 7873542, 26242183, 16585161, 7936666, 8631303, 19804365, 8621729, 10409742, 17210721, 23319808, 7624324, 22177953, 15175348
S218 Phosphorylation O15530 (PDPK1) neXtProt 15175348
S218 Phosphorylation P00540 (MOS) neXtProt 7873542
S218 Phosphorylation P04049 (RAF1) neXtProt   phospho.ELM   PhosphoSitePlus   Signor 11042690, 8131746, 8621729, ...10359597, 16585161
S218 Phosphorylation P10398 (ARAF) neXtProt   PhosphoSitePlus   Signor 8621729
S218 Phosphorylation P15056 (BRAF) neXtProt   PhosphoSitePlus 17667937, 17210721, 8621729
S218 Phosphorylation P41279 (MAP3K8) neXtProt   phospho.ELM   PhosphoSitePlus   Signor 8631303, 15466476, 19804365
S218 Phosphorylation P53350 (PLK1) PhosphoSitePlus 26242183
S218 Phosphorylation Q13233 (MAP3K1) neXtProt 8034665
S218 Phosphorylation Q5TCX8 (MAP3K21) neXtProt   Signor 23319808
S222 Phosphorylation neXtProt   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P   Signor   UniProt 27169363, 10359597, 29753091, ...17192257, 10037701, 20956560, 17667937, 8034665, 29433126, 33808483, 8952507, 8131746, 15466476, 11042690, 21793567, 14993270, 7873542, 26242183, 16585161, 8631303, 19804365, 8621729, 17210721, 8039496, 23319808, 7624324, 22177953, 15175348
S222 Phosphorylation O15530 (PDPK1) neXtProt   PhosphoSitePlus   Signor 15175348
S222 Phosphorylation P00540 (MOS) neXtProt 7873542
S222 Phosphorylation P04049 (RAF1) neXtProt   phospho.ELM   PhosphoSitePlus   Signor 8131746, 16585161, 8621729, ...10359597, 11042690
S222 Phosphorylation P10398 (ARAF) neXtProt   PhosphoSitePlus   Signor 8621729
S222 Phosphorylation P15056 (BRAF) neXtProt   PhosphoSitePlus 17667937, 17210721, 8621729
S222 Phosphorylation P41279 (MAP3K8) neXtProt   phospho.ELM   PhosphoSitePlus   Signor 8131746, 15466476, 19804365, ...8631303
S222 Phosphorylation P53350 (PLK1) PhosphoSitePlus 26242183
S222 Phosphorylation Q13233 (MAP3K1) neXtProt 8034665
S222 Phosphorylation Q5TCX8 (MAP3K21) neXtProt   Signor 23319808
T226 Phosphorylation PhosphoSitePlus
S231 Phosphorylation PhosphoSitePlus
S244 Phosphorylation Signor 8131746
S244 Phosphorylation P41279 (MAP3K8) Signor 8131746
S248 Phosphorylation Signor 8131746
S248 Phosphorylation P41279 (MAP3K8) Signor 8131746
T286 Phosphorylation neXtProt   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P   Signor   UniProt 18691976, 8114697, 18617556, ...25971971, 11684694, 29968772, 12609978
T286 Phosphorylation P06493 (CDK1) phospho.ELM   PhosphoSitePlus   Signor 12609978, 11684694, 8114697, ...25971971
T286 Phosphorylation P27361 (MAPK3) PhosphoSitePlus 25971971
T286 Phosphorylation P28482 (MAPK1) PhosphoSitePlus 25971971
T286 Phosphorylation Q00535 (CDK5) neXtProt 11684694
T292 Phosphorylation neXtProt   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P   Signor 27169363, 8114697, 25971971, ...17928366, 23735655, 22177953, 29804890, 29968772, 10567369, 8019005, 9351825, 12609978, 8013650, 14993270
T292 Phosphorylation P06493 (CDK1) PhosphoSitePlus   Signor 12609978, 25971971, 8114697
T292 Phosphorylation P27361 (MAPK3) neXtProt   PhosphoSitePlus 25971971, 8013650, 14993270
T292 Phosphorylation P28482 (MAPK1) neXtProt   phospho.ELM   PhosphoSitePlus 25971971, 10567369, 9351825
T292 Phosphorylation Q00535 (CDK5) PhosphoSitePlus 25971971
S298 Phosphorylation neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 17314031, 27169363, 17667937, ...11948406, 7873542, 16815847, 17928366, 16129686, 30971268, 18593598, 12876277, 23653349, 9351825, 19930650, 27542844, 23746211, 14993270
S298 Phosphorylation O75914 (PAK3) neXtProt 12876277
S298 Phosphorylation Q02750 (MAP2K1) neXtProt 7873542
S298 Phosphorylation Q13153 (PAK1) neXtProt   phospho.ELM   PhosphoSitePlus   Signor 16129686, 11948406, 9351825, ...12876277, 18593598, 14993270
S299 Phosphorylation PhosphoSitePlus
Y300 Phosphorylation neXtProt   PhosphoSitePlus 7873542
Y300 Phosphorylation Q02750 (MAP2K1) neXtProt 7873542
K344 Ubiquitination PhosphoSitePlus
K353 Ubiquitination PhosphoSitePlus
K362 Acetylation PhosphoSitePlus
S377 Phosphorylation PhosphoSitePlus
S385 Phosphorylation PhosphoSitePlus
T386 Phosphorylation neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor 27169363, 29968772, 10567369, ...8013650
T386 Phosphorylation P27361 (MAPK3) neXtProt 8013650
T386 Phosphorylation P28482 (MAPK1) neXtProt   phospho.ELM   PhosphoSitePlus   Signor 10567369
T388 Phosphorylation PhosphoSitePlus   RLIMS-P 27169363
Site PTM Type PTM Enzyme Score Source PMID
S218 Phosphorylation HPRD   PRO 8394352, 7731720, 8157000, ...8131746, 15466214, 8621729, 10409742
S218 Phosphorylation P04049 (RAF1) HPRD 7731720, 8157000, 8621729, ...10409742, 8394352
S218 Phosphorylation P10398 (ARAF) HPRD 7731720, 8157000, 8621729, ...8394352
S218 Phosphorylation P15056 (BRAF) HPRD 7731720, 8157000, 8621729, ...8394352
S222 Phosphorylation HPRD   PRO 8394352, 7731720, 8157000, ...8131746, 15466214, 8621729
S222 Phosphorylation P04049 (RAF1) HPRD 7731720, 8157000, 8621729, ...8394352
S222 Phosphorylation P10398 (ARAF) HPRD 7731720, 8157000, 8621729, ...8394352
S222 Phosphorylation P15056 (BRAF) HPRD 7731720, 8157000, 8621729, ...8394352
S231 Phosphorylation HPRD 18691976
T286 Phosphorylation HPRD 11684694, 18691976
T286 Phosphorylation P04049 (RAF1) HPRD 11684694, 18691976
T286 Phosphorylation Q00535 (CDK5) HPRD 11684694, 18691976
T292 Phosphorylation HPRD 8157000, 12594221
T292 Phosphorylation P04049 (RAF1) HPRD 8157000
T292 Phosphorylation P28482 (MAPK1) HPRD 8157000, 12594221
S298 Phosphorylation HPRD 9351825
S298 Phosphorylation Q13153 (PAK1) HPRD 9351825
S304 Phosphorylation HPRD 8226933
S304 Phosphorylation Q02750 (MAP2K1) HPRD 8226933
S385 Phosphorylation HPRD 18691976, 20058876
T386 Phosphorylation HPRD 8157000, 18691976
T386 Phosphorylation P04049 (RAF1) HPRD 8157000, 18691976


Q02750 (MAP2K1) as PTM Enzyme



Substrate Site Score Source PMID
O75676-2 (RPS6KA4) S196 HPRD 10806207
O75676-2 (RPS6KA4) S343 HPRD 10806207, 18691976, 19651622, ...20068231
O75676-1 (RPS6KA4) S347 HPRD 10806207, 18691976, 19651622, ...16964243, 20068231
O75676-2 (RPS6KA4) S360 HPRD 10806207
O75676-2 (RPS6KA4) T562 HPRD 10806207
O75676-1 (RPS6KA4) T568 HPRD 10806207
P04049 (RAF1) S338 PhosphoSitePlus 18775988
P05787 (KRT8) S74 PhosphoSitePlus 15939799
P15172 (MYOD1) Y156 RLIMS-P+ 21454680
P17542 (TAL1) S122 PhosphoSitePlus   Signor 11904294
P27361 (MAPK3) T202 neXtProt   phospho.ELM   PhosphoSitePlus   Signor 16585161, 8626767, 15939799, ...11971971, 9677429, 8226933
P27361 (MAPK3) Y204 neXtProt   phospho.ELM   PhosphoSitePlus   Signor 16585161, 8626767, 15939799, ...16153436, 11971971, 9677429, 8226933
P27361 (MAPK3) T207 PhosphoSitePlus 26823016
P27361 (MAPK3) Y210 PhosphoSitePlus 26823016
P28482 (MAPK1) T185 neXtProt   PhosphoSitePlus   Signor 11971971, 9677429, 21768338, ...21336309
P28482 (MAPK1) Y187 HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   Signor 17016520, 18691976, 18578522, ...17389395, 21768338, 21336309, 18452278, 19651622, 18669648, 19415658, 16153436, 18707149, 11971971, 19901323, 9677429, 20068231, 16497976
P32121 (ARRB2) T382 Signor 28169830
P35568 (IRS1) S307 Signor 11160134
P49841 (GSK3B) Y216 neXtProt   phospho.ELM   PhosphoSitePlus   Signor 15020233
P55211-1 (CASP9) T125 HPRD 12792650, 18669648
Q00613 (HSF1) S326 PhosphoSitePlus 25679764
Q02750 (MAP2K1) S298 neXtProt 7873542
Q02750 (MAP2K1) Y300 neXtProt 7873542
Q02750-1 (MAP2K1) S304 HPRD 8226933


Proteoforms

PRO ID (Short Label) Sites PTM Enzyme Source PMID
PR:000048292 (hMAP2K1/InitMet-/Phos:1) pS218,pS222 PRO
PR:000048293 (hMAP2K1/InitMet-/Phos:2) pS218,pS222,pT286,pT292 PRO
PR:000048294 (hMAP2K1/InitMet-/Phos:3) pS218,pS222,pT292 PRO
PR:000048295 (hMAP2K1/InitMet-/Phos:4) pT286,pT292 PRO
PR:000026220 (hMAP2K1/iso:1/Phos:1) pS218,pS222 PRO 8131746


PTM-dependent PPI

PTM type Substrate Site Interactant Association type Source PMID
Phosphorylation Q02750 (MAP2K1) S298 P28482 (MAPK1) increased_association eFIP 17928366
Phosphorylation Q02750 (MAP2K1) T292 P60484 (PTEN) decreased_association eFIP 23453810
Phosphorylation Q02750 (MAP2K1) S298 P28482 (MAPK1) increased_association eFIP 14993270


PTM sites affected in variants

Site Variant Source PMID Disease [Sample source]
T23 P23 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
T55 P55 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
K57 N57 Biomuta [ cosmic ] DOID:10283 / prostate cancer [ cosmic ] DOID:3571 / liver cancer [ cosmic ] DOID:1324 / lung cancer [ civic, cosmic, icgc ] DOID:2531 / hematologic cancer [ cosmic ] DOID:4606 / bile duct cancer [ cosmic ] DOID:4159 / skin cancer [ cosmic, icgc ] DOID:2394 / ovarian cancer [ cosmic ] DOID:1793 / pancreatic cancer [ cosmic ] DOID:10534 / stomach cancer [ cosmic ] DOID:11934 / head and neck cancer [ icgc ] DOID:9256 / colorectal cancer [ cosmic ] DOID:8618 / oral cavity cancer [ cosmic ]
K57 R57 Biomuta DOID:2531 / hematologic cancer [ cosmic ]
K57 T57 Biomuta DOID:1324 / lung cancer [ cosmic, icgc ] DOID:2531 / hematologic cancer [ cosmic ] DOID:9256 / colorectal cancer [ cosmic ] DOID:10534 / stomach cancer [ cosmic ] DOID:8618 / oral cavity cancer [ cosmic ] DOID:9253 / gastrointestinal stromal tumor [ cosmic ]
K57 E57 Biomuta DOID:4606 / bile duct cancer [ cosmic ] DOID:2531 / hematologic cancer [ cosmic ] DOID:1612 / breast cancer [ cosmic ] DOID:4159 / skin cancer [ cosmic ] DOID:9256 / colorectal cancer [ cosmic ] DOID:1319 / brain cancer [ cosmic ]
K64 E64 Biomuta DOID:10534 / stomach cancer [ cosmic ]
S72 G72 Biomuta DOID:10534 / stomach cancer [ cosmic ]
K192 N192 Biomuta DOID:1781 / thyroid gland cancer [ cosmic ] DOID:1781 / thyroid cancer [ icgc ]
S194 A194 Biomuta DOID:1324 / lung cancer [ cosmic ]
S212 N212 Biomuta DOID:363 / uterine cancer [ cosmic ] DOID:9256 / colorectal cancer [ cosmic ]
S218 F218 Biomuta DOID:10534 / stomach cancer [ cosmic ]
S231 L231 Biomuta DOID:363 / uterine cancer [ cosmic, icgc ] DOID:1612 / breast cancer [ cosmic ] DOID:10534 / stomach cancer [ cosmic ] DOID:4159 / skin cancer [ cosmic ] DOID:9256 / colorectal cancer [ cosmic, icgc ]
S377 F377 Biomuta DOID:1612 / breast cancer [ cosmic ]