iPTMnet Report for Q00987 (MDM2)


Protein Information

Cytoscape View


UniProt AC / UniProt ID Q00987 / MDM2_HUMAN
Protein Name E3 ubiquitin-protein ligase Mdm2
Gene Name Name: MDM2
Organism Homo sapiens (Human)

PRO ID PR:Q00987
DAG View
PRO Name E3 ubiquitin-protein ligase Mdm2 (human)
Definition An E3 ubiquitin-protein ligase Mdm2 that is encoded in the genome of human.
Short Label hMDM2
Category organism-gene

Interactive Sequence View

Select/align proteoforms across species


Q00987 (MDM2) as Substrate



Site PTM Type PTM Enzyme Score Source PMID
Ubiquitination PRO
T4 Phosphorylation RLIMS-P 26531827
S17 Phosphorylation PhosphoSitePlus   RLIMS-P 10751633, 27050388
K36 Ubiquitination PhosphoSitePlus
K39 Ubiquitination PhosphoSitePlus
K45 Ubiquitination PhosphoSitePlus
Y60 Phosphorylation PhosphoSitePlus
K70 Ubiquitination PhosphoSitePlus
C77 S-Nitrosylation dbSNO 22178444, 15688001
K94 Ubiquitination PhosphoSitePlus
K98 Ubiquitination PhosphoSitePlus
S118 Phosphorylation PhosphoSitePlus   RLIMS-P   Signor 24567357, 20708156
S118 Phosphorylation P48730 (CSNK1D) PhosphoSitePlus   Signor 20708156
S121 Phosphorylation PhosphoSitePlus   Signor 20708156
S121 Phosphorylation P48730 (CSNK1D) PhosphoSitePlus   Signor 20708156
K146 Ubiquitination PhosphoSitePlus
S148 Phosphorylation PhosphoSitePlus
S157 Phosphorylation neXtProt   phospho.ELM   PhosphoSitePlus 15688025
S157 Phosphorylation P49137 (MAPKAPK2) neXtProt   PhosphoSitePlus 15688025
T158 Phosphorylation PhosphoSitePlus 23132866
T158 Phosphorylation Q9NQU5 (PAK6) PhosphoSitePlus 23132866
S160 Phosphorylation PhosphoSitePlus
S166 Phosphorylation IntAct   neXtProt   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P   Signor   UniProt 26636645, 28993697, 30177839, ...25431954, 27986870, 19166854, 19751709, 18332867, 32681102, 22955948, 16103882, 25482947, 17341630, 17107963, 30622244, 15688025, 15625077, 34992144, 21909130, 23186163, 32453417, 24762959, 23203124, 11715018, 19008219, 23843462, 11960368, 11504915, 19690332, 15001356, 26993775, 26196320, 27617579, 33400740, 17565390, 27591897, 15527798, 25392082, 22525275, 20708156, 15026814, 26984736, 18467333, 28928040, 36149602, 31534052, 16983334, 15169778, 32945383, 27659528, 19756449, 19306413, 19247369, 11850850, 26462020, 20840854, 28005077, 18359851, 28468838
S166 Phosphorylation O00141 (SGK1) neXtProt 19756449
S166 Phosphorylation O43293 (DAPK3) neXtProt   phospho.ELM   PhosphoSitePlus   Signor 15001356
S166 Phosphorylation P11309 (PIM1) neXtProt   PhosphoSitePlus   Signor 18467333
S166 Phosphorylation P31749 (AKT1) neXtProt   PhosphoSitePlus   Signor 15169778, 15527798, 11850850, ...11960368, 11715018, 11504915
S166 Phosphorylation P31751 (AKT2) Signor 11504915
S166 Phosphorylation P48730 (CSNK1D) PhosphoSitePlus 20708156
S166 Phosphorylation P49137 (MAPKAPK2) neXtProt   PhosphoSitePlus   Signor 15688025
S166 Phosphorylation Q9P1W9 (PIM2) neXtProt 18467333
T168 Phosphorylation PhosphoSitePlus
S172 Phosphorylation PhosphoSitePlus 20708156
S172 Phosphorylation P48730 (CSNK1D) PhosphoSitePlus 20708156
S176 Phosphorylation PhosphoSitePlus 20708156
S176 Phosphorylation P48730 (CSNK1D) PhosphoSitePlus 20708156
K182 Acetylation PhosphoSitePlus
K185 Acetylation PhosphoSitePlus
S186 Phosphorylation IEDB   IntAct   neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor 32897882, 23132866, 27659528, ...19166854, 11715018, 25392082, 22525275, 32681102, 11923280, 18467333, 11504915, 18359851, 15001356
S186 Phosphorylation O43293 (DAPK3) PhosphoSitePlus 15001356
S186 Phosphorylation P11309 (PIM1) neXtProt   PhosphoSitePlus   Signor 18467333
S186 Phosphorylation P31749 (AKT1) neXtProt   PhosphoSitePlus   Signor 11923280, 11504915, 11715018
S186 Phosphorylation P31751 (AKT2) Signor 11504915
S186 Phosphorylation Q9NQU5 (PAK6) PhosphoSitePlus 23132866
S186 Phosphorylation Q9P1W9 (PIM2) neXtProt 18467333
S188 Phosphorylation IEDB   IntAct   neXtProt   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P   Signor 32897882, 15169778, 19166854, ...15527798
S188 Phosphorylation P31749 (AKT1) neXtProt   PhosphoSitePlus   Signor 15169778, 15527798
S190 Phosphorylation IEDB   PhosphoSitePlus 32897882
S192 Phosphorylation PhosphoSitePlus
S196 Phosphorylation PhosphoSitePlus
T216 Phosphorylation neXtProt   RLIMS-P 25431954, 11983168, 11359766
T218 Phosphorylation PRO   PhosphoSitePlus
S220 Phosphorylation PhosphoSitePlus 20708156
S220 Phosphorylation P48730 (CSNK1D) PhosphoSitePlus 20708156
S229 Phosphorylation PhosphoSitePlus 20708156
S229 Phosphorylation P48730 (CSNK1D) PhosphoSitePlus 20708156
S240 Phosphorylation phospho.ELM   PhosphoSitePlus   UniProt 20708156, 12167711
S240 Phosphorylation P48730 (CSNK1D) PhosphoSitePlus 20708156
S242 Phosphorylation phospho.ELM   PhosphoSitePlus   UniProt 12167711
S246 Phosphorylation phospho.ELM   PhosphoSitePlus   Signor   UniProt 20708156, 12167711
S246 Phosphorylation P48730 (CSNK1D) Signor 20708156
S253 Phosphorylation PhosphoSitePlus   Signor 20708156, 12167711
S253 Phosphorylation P48730 (CSNK1D) PhosphoSitePlus   Signor 20708156, 12167711
S256 Phosphorylation PhosphoSitePlus
S260 Phosphorylation IntAct   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 19833129, 12383858, 12167711, ...26754547, 20708156, 16335531
S260 Phosphorylation P48730 (CSNK1D) PhosphoSitePlus 20708156
S260 Phosphorylation P53350 (PLK1) PhosphoSitePlus   Signor 19833129, 12383858
S260 Phosphorylation P68400 (CSNK2A1) PhosphoSitePlus 16335531
S262 Phosphorylation phospho.ELM   PhosphoSitePlus   Signor   UniProt 20708156, 12167711
S262 Phosphorylation P48730 (CSNK1D) PhosphoSitePlus   Signor 20708156, 12167711
S269 Phosphorylation phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor 10561590, 12167711, 20708156, ...16335531, 15703839
S269 Phosphorylation P48730 (CSNK1D) PhosphoSitePlus   Signor 20708156, 12167711
S269 Phosphorylation P68400 (CSNK2A1) PhosphoSitePlus 16335531, 15703839
Y276 Phosphorylation PhosphoSitePlus   RLIMS-P   Signor 16702947, 21081495
Y276 Phosphorylation P00519 (ABL1) PhosphoSitePlus   Signor 16702947, 21081495
T279 Phosphorylation PhosphoSitePlus 20708156
T279 Phosphorylation P48730 (CSNK1D) PhosphoSitePlus 20708156
Y281 Phosphorylation PhosphoSitePlus 25624478
Y281 Phosphorylation P12931 (SRC) PhosphoSitePlus 25624478
S286 Phosphorylation PhosphoSitePlus
T288 Phosphorylation PhosphoSitePlus   RLIMS-P 26531827
S290 Phosphorylation PhosphoSitePlus 20708156
S290 Phosphorylation P48730 (CSNK1D) PhosphoSitePlus 20708156
Y302 Phosphorylation PhosphoSitePlus 25624478
Y302 Phosphorylation P12931 (SRC) PhosphoSitePlus 25624478
T306 Phosphorylation PhosphoSitePlus   RLIMS-P 26531827
S307 Phosphorylation PhosphoSitePlus   RLIMS-P 26531827
K334 Ubiquitination PhosphoSitePlus
K336 Ubiquitination PhosphoSitePlus
K338 Ubiquitination PhosphoSitePlus
S342 Phosphorylation PhosphoSitePlus   RLIMS-P 16943424, 20708156
S342 Phosphorylation P48730 (CSNK1D) PhosphoSitePlus 20708156
K344 Ubiquitination PhosphoSitePlus
K346 Ubiquitination PhosphoSitePlus
N349 N-Glycosylation GlyGen 29671580
S350 Phosphorylation PhosphoSitePlus 20708156
S350 Phosphorylation P48730 (CSNK1D) PhosphoSitePlus 20708156
T351 Phosphorylation PhosphoSitePlus 20708156
T351 Phosphorylation P48730 (CSNK1D) PhosphoSitePlus 20708156
K363 Ubiquitination PhosphoSitePlus
K364 Ubiquitination PhosphoSitePlus
T365 Phosphorylation PhosphoSitePlus 20708156
T365 Phosphorylation P48730 (CSNK1D) PhosphoSitePlus 20708156
S373 Phosphorylation PhosphoSitePlus 20708156
S373 Phosphorylation P48730 (CSNK1D) PhosphoSitePlus 20708156
S386 Phosphorylation neXtProt   PRO   PhosphoSitePlus   RLIMS-P   Signor   UniProt 20708156, 27462439, 19816404
S386 Phosphorylation P48730 (CSNK1D) PhosphoSitePlus 20708156
S386 Phosphorylation Q13315 (ATM) neXtProt   PhosphoSitePlus   Signor 19816404
S388 Phosphorylation PhosphoSitePlus
Y394 Phosphorylation phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor 14757045, 16702947, 12110584
Y394 Phosphorylation A0A173G4P4 (Abl fusion) phospho.ELM 14757045, 16702947, 12110584
Y394 Phosphorylation P00519 (ABL1) PhosphoSitePlus   Signor 16702947, 12110584
S395 Phosphorylation neXtProt   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P+   RLIMS-P   Signor   UniProt 26636645, 33400740, 32453417, ...17936559, 16702947, 27215386, 30745421, 23405218, 22976441, 27462439, 23150762, 22264786, 11331603, 19816404, 23603988
S395 Phosphorylation Q13315 (ATM) neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P+   Signor 11331603, 22976441, 17936559
Y405 Phosphorylation PhosphoSitePlus   Signor 16702947, 21081495
Y405 Phosphorylation P00519 (ABL1) PhosphoSitePlus   Signor 16702947, 21081495
S406 Phosphorylation PhosphoSitePlus
S407 Phosphorylation neXtProt   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P   Signor   UniProt 14654783, 19816404
S407 Phosphorylation Q13535 (ATR) neXtProt   phospho.ELM   PhosphoSitePlus   Signor 14654783
T419 Phosphorylation PRO   PhosphoSitePlus   UniProt 20708156, 19816404
T419 Phosphorylation P48730 (CSNK1D) PhosphoSitePlus 20708156
S425 Phosphorylation PhosphoSitePlus   UniProt 19816404
S429 Phosphorylation neXtProt   PhosphoSitePlus   RLIMS-P   Signor   UniProt 32350255, 19816404, 27462439
S429 Phosphorylation Q13315 (ATM) neXtProt   PhosphoSitePlus   Signor 19816404
K446 Sumoylation PhosphoSitePlus
K446 Ubiquitination PhosphoSitePlus
K454 Ubiquitination PhosphoSitePlus
K466 Ubiquitination PhosphoSitePlus
K467 Ubiquitination PhosphoSitePlus
K473 Ubiquitination PhosphoSitePlus
Site PTM Type PTM Enzyme Score Source PMID
N349 N-Glycosylation PRO 29671580
Site PTM Type PTM Enzyme Score Source PMID
S166 Phosphorylation HPRD 15527798
S166 Phosphorylation P31749 (AKT1) HPRD 15527798
S172 Phosphorylation HPRD 11504915, 19664995
S172 Phosphorylation P31749 (AKT1) HPRD 11504915, 19664995
S192 Phosphorylation HPRD 11504915
S192 Phosphorylation P31749 (AKT1) HPRD 11504915
Y400 Phosphorylation HPRD 12110584
Y400 Phosphorylation P00519 (ABL1) HPRD 12110584
S401 Phosphorylation HPRD 11331603
S401 Phosphorylation Q13315 (ATM) HPRD 11331603


Proteoforms

PRO ID (Short Label) Sites PTM Enzyme Source PMID
PR:000048357 (hMDM2/Phos:2) pS166,pS188 PRO
PR:000048358 (hMDM2/Phos:3) pS166,pS188,pT218 PRO
PR:000048359 (hMDM2/Phos:4) pS166,pS188,pS386,pS395,pS407,pT419 PRO
PR:000048360 (hMDM2/UbiqLys+) ub PRO


PTM-dependent PPI

PTM type Substrate Site Interactant Association type Source PMID
Phosphorylation P04637 (TP53) S15 Q00987 (MDM2) inhibited_association eFIP 10601022
Phosphorylation P04637 (TP53) T18 Q00987 (MDM2) inhibited_association eFIP 19332559
Phosphorylation P04637 (TP53) T18 Q00987 (MDM2) decreased_association eFIP 20158876
Phosphorylation P04637 (TP53) S315 Q00987 (MDM2) inhibited_association eFIP 26387611
Phosphorylation P04637 (TP53) S20 Q00987 (MDM2) inhibited_association eFIP 21338495
Phosphorylation P04637 (TP53) S15 Q00987 (MDM2) inhibited_association eFIP 10656682
Phosphorylation P04637 (TP53) T18 Q00987 (MDM2) inhibited_association eFIP 19357310
Phosphorylation P04637 (TP53) T18 Q00987 (MDM2) inhibited_association eFIP 21386980
Phosphorylation P04637 (TP53) S106 Q00987 (MDM2) decreased_association eFIP 23201157
Phosphorylation P04637 (TP53) S15 Q00987 (MDM2) decreased_association eFIP 30476192
Phosphorylation P04637 (TP53) S15 Q00987 (MDM2) increased_association eFIP 16632641
Phosphorylation P04637 (TP53) S15 Q00987 (MDM2) inhibited_association eFIP 10435622
Phosphorylation P04637 (TP53) S15 Q00987 (MDM2) inhibited_association eFIP 26540344
Phosphorylation P04637 (TP53) S20 Q00987 (MDM2) inhibited_association eFIP 21317463
Phosphorylation P04637 (TP53) S20 Q00987 (MDM2) inhibited_association eFIP 31594538
Phosphorylation P04637 (TP53) S20 Q00987 (MDM2) inhibited_association eFIP 31979361
Phosphorylation P04637 (TP53) S37 Q00987 (MDM2) inhibited_association eFIP 23437037
Phosphorylation P04637 (TP53) S20 Q00987 (MDM2) decreased_association eFIP 10597229
Phosphorylation P06400 (RB1) S567 Q00987 (MDM2) increased_association eFIP 28050229
Phosphorylation P25098 (GRK2) S670 Q00987 (MDM2) unknown eFIP 33806062
Phosphorylation Q00987 (MDM2) S166 P15692 (VEGFA) unknown eFIP 27591897
Phosphorylation Q00987 (MDM2) S186 P11309 (PIM1) unknown eFIP 19166854
Phosphorylation Q9UER7 (DAXX) S564 Q00987 (MDM2) unknown eFIP 23405218
Phosphorylation Q9Y5J3 (HEY1) S68 Q00987 (MDM2) decreased_association eFIP 27129302


Proteoform PPIs

Protein 1 Relation Protein 2 Source PMID
PR:P29590-5 (hPML/iso:5) Interaction PR:Q00987 (hMDM2) PRO 12915590


PTM sites affected in variants

Site Variant Source PMID Disease [Sample source]
S17 L17 Biomuta DOID:4159 / skin cancer [ cosmic ]
K36 R36 Biomuta DOID:1781 / thyroid gland cancer [ cosmic ] DOID:1781 / thyroid cancer [ icgc ]
K70 Q70 Biomuta DOID:184 / bone cancer [ cosmic ]
K70 R70 Biomuta DOID:4159 / skin cancer [ cosmic ]
S121 F121 Biomuta DOID:1319 / brain cancer [ icgc ] DOID:1612 / breast cancer [ cosmic ]
S160 F160 Biomuta DOID:1324 / lung cancer [ cosmic ]
S166 F166 Biomuta DOID:1324 / lung cancer [ cosmic ]
S176 F176 Biomuta DOID:1612 / breast cancer [ cosmic ] DOID:1324 / lung cancer [ cosmic ]
K185 R185 Biomuta DOID:1612 / breast cancer [ cosmic ] DOID:2531 / hematologic cancer [ cosmic ]
S190 F190 Biomuta DOID:4159 / skin cancer [ cosmic ]
S192 F192 Biomuta DOID:4159 / skin cancer [ cosmic ]
T216 S216 Biomuta DOID:5041 / esophageal cancer [ cosmic ]
T218 M218 Biomuta DOID:10534 / stomach cancer [ cosmic, icgc ] DOID:5041 / esophageal cancer [ cosmic ]
S220 * Biomuta DOID:1793 / pancreatic cancer [ cosmic ]
S220 W220 Biomuta DOID:1612 / breast cancer [ cosmic ]
S220 L220 Biomuta DOID:363 / uterine cancer [ cosmic, icgc ] DOID:4159 / skin cancer [ cosmic ]
S229 N229 Biomuta DOID:9256 / colorectal cancer [ cosmic ] DOID:1319 / brain cancer [ cosmic ]
S240 L240 Biomuta DOID:11054 / urinary bladder cancer [ cosmic, icgc ]
S253 Y253 Biomuta DOID:363 / uterine cancer [ icgc ] DOID:8618 / oral cavity cancer [ cosmic ] DOID:11934 / head and neck cancer [ icgc ]
S256 * Biomuta DOID:363 / uterine cancer [ cosmic ] DOID:4606 / bile duct cancer [ cosmic ]
S262 N262 Biomuta DOID:263 / kidney cancer [ cosmic ]
S269 * Biomuta DOID:11054 / urinary bladder cancer [ cosmic ]
Y276 H276 Biomuta DOID:1793 / pancreatic cancer [ cosmic ]
Y276 N276 Biomuta DOID:4159 / skin cancer [ cosmic ]
Y276 C276 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
T279 P279 Biomuta DOID:10534 / stomach cancer [ cosmic ]
Y281 H281 Biomuta DOID:1319 / brain cancer [ cosmic ]
T306 S306 Biomuta DOID:8618 / oral cavity cancer [ cosmic ]
S307 L307 Biomuta DOID:4159 / skin cancer [ cosmic ]
K334 Q334 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
K364 T364 Biomuta DOID:363 / uterine cancer [ cosmic ]
T365 N365 Biomuta DOID:11615 / penile cancer [ cosmic ]
Y405 C405 Biomuta DOID:1324 / lung cancer [ cosmic ]
S407 R407 Biomuta DOID:2998 / testicular cancer [ cosmic ]
S407 G407 Biomuta DOID:1612 / breast cancer [ cosmic ]
S407 N407 Biomuta DOID:363 / uterine cancer [ cosmic ]
T419 I419 Biomuta [ cosmic ]
S425 C425 Biomuta DOID:3571 / liver cancer [ cosmic ]
S429 R429 Biomuta DOID:363 / uterine cancer [ cosmic ] DOID:10283 / prostate cancer [ cosmic ]