iPTMnet Report for P84022 (SMAD3)


Protein Information

Cytoscape View


UniProt AC / UniProt ID P84022 / SMAD3_HUMAN
Protein Name Mothers against decapentaplegic homolog 3
Gene Name Name: SMAD3
Synonyms:MADH3;
Organism Homo sapiens (Human)

PRO ID PR:P84022
DAG View
PRO Name mothers against decapentaplegic homolog 3 (human)
Definition A smad3 that is encoded in the genome of human.
Short Label hSMAD3
Category organism-gene

Interactive Sequence View

Select/align proteoforms across species


P84022 (SMAD3) as Substrate



Site PTM Type PTM Enzyme Score Source PMID
Ubiquitination PRO
S2 Acetylation UniProt 19413330, 22814378
S3 Phosphorylation RLIMS-P 26497676, 30356176
T8 Phosphorylation neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 15241418, 19114991, 20736297, ...15520018, 27906182
T8 Phosphorylation P11802 (CDK4) neXtProt   phospho.ELM   PhosphoSitePlus   Signor 15241418, 20736297, 15520018, ...19114991
T8 Phosphorylation P24941 (CDK2) neXtProt   phospho.ELM   Signor 15241418, 15520018, 19114991
K13 Ubiquitination PhosphoSitePlus
K19 Acetylation PhosphoSitePlus
K20 Acetylation PhosphoSitePlus
K29 Acetylation PhosphoSitePlus
K33 Ubiquitination UniProt 21947082
K36 Sumoylation PhosphoSitePlus
S37 Phosphorylation PhosphoSitePlus
K53 Methylation PhosphoSitePlus
K63 Ubiquitination PhosphoSitePlus
T66 Phosphorylation RLIMS-P   Signor 18172167
T66 Phosphorylation P49841 (GSK3B) Signor 18172167
S78 Phosphorylation PhosphoSitePlus
K81 Ubiquitination PhosphoSitePlus   UniProt 21947082
Y88 Phosphorylation PhosphoSitePlus
K117 Acetylation PhosphoSitePlus
C121 Methylation IEDB 31844290
Y125 Phosphorylation PhosphoSitePlus
T132 Phosphorylation PhosphoSitePlus
T179 Phosphorylation neXtProt   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P   Signor   UniProt 29524413, 20736297, 28032440, ...27906182, 29097203, 19914168, 29137393, 10197981, 19914161, 26497676, 15241418, 24080014, 15520018, 16156666, 23077590, 19920136, 26297431, 19114991, 22927969, 30251686, 19218245
T179 Phosphorylation P11802 (CDK4) neXtProt   phospho.ELM   PhosphoSitePlus   Signor 15241418, 20736297, 19114991
T179 Phosphorylation P24941 (CDK2) neXtProt   phospho.ELM   Signor 15241418, 19114991
T179 Phosphorylation P28482 (MAPK1) phospho.ELM   PhosphoSitePlus   Signor 10197981, 16156666
T179 Phosphorylation P31749 (AKT1) PhosphoSitePlus 26297431
T179 Phosphorylation P49336 (CDK8) PhosphoSitePlus   Signor 19914161, 19914168
T179 Phosphorylation P50750 (CDK9) PhosphoSitePlus   Signor 19914161, 19914168
S204 Phosphorylation neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P+   RLIMS-P   Signor   UniProt 19458083, 26956045, 23077590, ...15241418, 30542739, 10197981, 20659902, 26297431, 27906182, 28032440, 19914168, 20736297, 15520018, 19914161, 16156666, 24080014, 29057934, 19218245
S204 Phosphorylation P11802 (CDK4) neXtProt   PhosphoSitePlus 15241418, 20736297
S204 Phosphorylation P27361 (MAPK3) RLIMS-P+ 24080014
S204 Phosphorylation P28482 (MAPK1) phospho.ELM   PhosphoSitePlus   RLIMS-P+   Signor 19914168, 15241418, 10197981, ...19914161, 24080014, 16156666
S204 Phosphorylation Q14680 (MELK) neXtProt 20659902
S204 Phosphorylation Q16539 (MAPK14) Signor 15520018
S208 Phosphorylation neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 29499695, 30171053, 23991075, ...26919443, 20736297, 28032440, 33676893, 19914168, 10197981, 19914161, 26497676, 15241418, 24080014, 15520018, 31722981, 16156666, 23077590, 26297431, 27906182, 30899443, 16528675, 19218245
S208 Phosphorylation P11802 (CDK4) neXtProt   PhosphoSitePlus 15241418, 20736297
S208 Phosphorylation P28482 (MAPK1) phospho.ELM   PhosphoSitePlus   Signor 15241418, 19914168, 10197981, ...19914161, 16156666
S208 Phosphorylation P49336 (CDK8) PhosphoSitePlus   Signor 19914161, 19914168
S208 Phosphorylation P50750 (CDK9) PhosphoSitePlus   Signor 19914161, 19914168
S208 Phosphorylation Q16539 (MAPK14) Signor 15520018
S213 Phosphorylation neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 19914168, 23991075, 10197981, ...15241418, 26297431, 27906182, 20736297, 24080014, 28471448, 15520018, 19914161, 28032440, 19114991, 16528675
S213 Phosphorylation P11802 (CDK4) neXtProt   phospho.ELM   PhosphoSitePlus   Signor 15241418, 20736297, 19114991
S213 Phosphorylation P24941 (CDK2) neXtProt   phospho.ELM   Signor 15241418, 19114991
S213 Phosphorylation P28482 (MAPK1) phospho.ELM   PhosphoSitePlus   Signor 10197981, 19914161, 19914168
S213 Phosphorylation P49336 (CDK8) PhosphoSitePlus   Signor 19914161, 19914168
S213 Phosphorylation P50750 (CDK9) PhosphoSitePlus   Signor 19914161, 19914168
S264 Phosphorylation PhosphoSitePlus
S275 Phosphorylation PhosphoSitePlus
S309 Phosphorylation PhosphoSitePlus
C320 Methylation IEDB 31844290
K333 Acetylation PhosphoSitePlus
K333 Methylation PhosphoSitePlus
K378 Acetylation PhosphoSitePlus   UniProt 16862174
T388 Phosphorylation PhosphoSitePlus   RLIMS-P 24485922
T412 Phosphorylation PhosphoSitePlus
S416 Phosphorylation phospho.ELM   PhosphoSitePlus   UniProt 18669648, 23186163
S418 Phosphorylation neXtProt   PhosphoSitePlus   Signor   UniProt 18794808
S418 Phosphorylation P78368 (CSNK1G2) neXtProt   Signor 18794808
S422 Phosphorylation neXtProt   phospho.ELM   PhosphoSitePlus 9380693
S422 Phosphorylation P36897 (TGFBR1) neXtProt 9380693
S423 Phosphorylation neXtProt   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P 22995475, 30135653, 29321644, ...29179471, 28454099, 30363688, 32515734, 26919443, 27906182, 24312578, 24759784, 25878769, 33295886, 26497676, 30805303, 30274147, 9380693, 31924815
S423 Phosphorylation P36897 (TGFBR1) neXtProt 9380693
S425 Phosphorylation neXtProt   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P 22995475, 30135653, 29321644, ...29179471, 28454099, 30363688, 32515734, 26919443, 27906182, 24312578, 24759784, 25878769, 33295886, 26497676, 30805303, 30274147, 9380693, 31924815
S425 Phosphorylation P36897 (TGFBR1) neXtProt 9380693
Site PTM Type PTM Enzyme Score Source PMID
T8 Phosphorylation HPRD 15241418, 19413330, 20068231
T8 Phosphorylation P11802 (CDK4) HPRD 15241418, 19413330, 20068231
T8 Phosphorylation P24941 (CDK2) HPRD 15241418, 19413330, 20068231
K19 Acetylation PRO 17074756
T179 Phosphorylation HPRD 15241418, 10197981, 16156666
T179 Phosphorylation P11802 (CDK4) HPRD 15241418
T179 Phosphorylation P24941 (CDK2) HPRD 15241418
T179 Phosphorylation P28482 (MAPK1) HPRD 10197981, 16156666
S204 Phosphorylation HPRD 15241418, 10197981, 16156666
S204 Phosphorylation P11802 (CDK4) HPRD 15241418
S204 Phosphorylation P24941 (CDK2) HPRD 15241418
S204 Phosphorylation P28482 (MAPK1) HPRD 10197981, 16156666
S208 Phosphorylation HPRD 15241418, 14512875, 10197981, ...16156666
S208 Phosphorylation P11802 (CDK4) HPRD 15241418
S208 Phosphorylation P24941 (CDK2) HPRD 15241418
S208 Phosphorylation P28482 (MAPK1) HPRD 10197981, 16156666
S208 Phosphorylation Q16539 (MAPK14) HPRD 14512875
S213 Phosphorylation HPRD 15241418, 10197981, 14512875
S213 Phosphorylation P11802 (CDK4) HPRD 15241418
S213 Phosphorylation P24941 (CDK2) HPRD 15241418
S213 Phosphorylation P28482 (MAPK1) HPRD 10197981
S213 Phosphorylation Q16539 (MAPK14) HPRD 14512875
S416 Phosphorylation HPRD 18669648
S422 Phosphorylation HPRD 9380693
S422 Phosphorylation O43318 (MAP3K7) HPRD 9380693
S423 Phosphorylation HPRD   PRO 17074756, 9380693
S423 Phosphorylation O43318 (MAP3K7) HPRD 9380693
S425 Phosphorylation HPRD   PRO 17074756, 9380693
S425 Phosphorylation O43318 (MAP3K7) HPRD 9380693


Proteoforms

PRO ID (Short Label) Sites PTM Enzyme Source PMID
PR:000049285 (hSMAD3/Phos:1) pS423,pS425 PRO
PR:000049286 (hSMAD3/Phos:2) pT179,pS423,pS425 PRO
PR:000049287 (hSMAD3/UbiqLys+) ub PRO
PR:000026333 (hSMAD3/iso:1/Phos:1) pS423,pS425 PRO
PR:000036557 (hSMAD3/iso:1/AcPhos:1) acK19 PRO 17074756
PR:000036557 (hSMAD3/iso:1/AcPhos:1) pS423,pS425 PRO 17074756


PTM-dependent PPI

PTM type Substrate Site Interactant Association type Source PMID
Phosphorylation P84022 (SMAD3) T388 Q13485 (SMAD4) increased_association eFIP 24485922
Phosphorylation P84022 (SMAD3) T388 P49336 (CDK8) increased_association eFIP 24485922


Proteoform PPIs

Protein 1 Relation Protein 2 Source PMID
PR:000026333 (hSMAD3/iso:1/Phos:1) Interaction PR:Q13485 (hSMAD4) PRO 15350224, 9311995
PR:P84022-1 (hSMAD3/iso:1) Interaction PR:000025959 (hTGFBR1/iso:1/SigPep-/Phos:1) PRO 12154125
PR:P84022-1 (hSMAD3/iso:1) Interaction PR:O95405-1 (hZFYVE9/iso:1) PRO 12154125


PTM sites affected in variants

Site Variant Source PMID Disease [Sample source]
K36 N36 Biomuta DOID:363 / uterine cancer [ cosmic ]
K63 T63 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
T66 I66 Biomuta DOID:1319 / brain cancer [ cosmic ]
K81 N81 Biomuta DOID:1793 / pancreatic cancer [ cosmic ] DOID:9256 / colorectal cancer [ cosmic ]
Y88 C88 Biomuta DOID:10534 / stomach cancer [ cosmic ]
C121 F121 Biomuta DOID:3571 / liver cancer [ cosmic, icgc ]
S204 Y204 Biomuta DOID:1612 / breast cancer [ cosmic, icgc ]
S204 F204 Biomuta DOID:5041 / esophageal cancer [ cosmic ]
S264 Y264 Biomuta DOID:8618 / oral cavity cancer [ cosmic ] DOID:11934 / head and neck cancer [ icgc ]
S264 P264 Biomuta [ cosmic ]
S264 F264 Biomuta [ cosmic ] DOID:1115 / sarcoma [ icgc ]
S264 C264 Biomuta DOID:2394 / ovarian cancer [ cosmic ]
S309 N309 Biomuta DOID:4159 / skin cancer [ cosmic ]
C320 Y320 Biomuta DOID:9256 / colorectal cancer [ cosmic ] DOID:1319 / brain cancer [ icgc ]
K333 E333 Biomuta DOID:3571 / liver cancer [ cosmic, icgc ]
S416 F416 Biomuta DOID:4362 / cervical cancer [ cosmic, icgc ] DOID:1612 / breast cancer [ cosmic ] DOID:8618 / oral cavity cancer [ cosmic ] DOID:11934 / head and neck cancer [ icgc ] DOID:9256 / colorectal cancer [ cosmic ]
S418 I418 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
S418 N418 Biomuta DOID:10534 / stomach cancer [ cosmic, icgc ]
S422 F422 Biomuta DOID:1793 / pancreatic cancer [ cosmic ] DOID:4159 / skin cancer [ cosmic ] DOID:10534 / stomach cancer [ cosmic ] DOID:4606 / bile duct cancer [ cosmic ]
S422 Y422 Biomuta DOID:1793 / pancreatic cancer [ cosmic ] DOID:1324 / lung cancer [ cosmic ]
S423 I423 Biomuta DOID:1793 / pancreatic cancer [ cosmic ]
S423 T423 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
S423 R423 Biomuta DOID:1793 / pancreatic cancer [ cosmic ] DOID:1324 / lung cancer [ cosmic, icgc ]
S423 N423 Biomuta DOID:1793 / pancreatic cancer [ cosmic ]
S423 G423 Biomuta DOID:2394 / ovarian cancer [ cosmic ]
S425 Y425 Biomuta DOID:363 / uterine cancer [ cosmic, icgc ]
S425 C425 Biomuta DOID:8618 / oral cavity cancer [ cosmic ] DOID:1612 / breast cancer [ cosmic ] DOID:9256 / colorectal cancer [ cosmic ]
S425 F425 Biomuta DOID:1793 / pancreatic cancer [ cosmic ] DOID:9256 / colorectal cancer [ cosmic ]