Protein Information

Cytoscape View


UniProt AC / UniProt ID P54646 / AAPK2_HUMAN
Protein Name 5'-AMP-activated protein kinase catalytic subunit alpha-2
Gene Name Name: PRKAA2
Synonyms:AMPK,AMPK2;
Organism Homo sapiens (Human)

PRO ID PR:P54646
DAG View
PRO Name 5'-AMP-activated protein kinase catalytic subunit alpha-2 (human)
Definition A 5'-AMP-activated protein kinase catalytic subunit alpha-2 that is encoded in the genome of human.
Short Label hPRKAA2
Category organism-gene

Interactive Sequence View

Select/align proteoforms across species


P54646 (PRKAA2) as Substrate



Site PTM Type PTM Enzyme Score Source PMID
K41 Ubiquitination PhosphoSitePlus
K45 Ubiquitination PhosphoSitePlus
K69 Acetylation PhosphoSitePlus
K69 Ubiquitination PhosphoSitePlus
T85 Phosphorylation RLIMS-P 33898950
K141 Ubiquitination PhosphoSitePlus
S161 Phosphorylation PhosphoSitePlus
S165 Phosphorylation PhosphoSitePlus
T172 Phosphorylation Q96RR4 (CAMKK2) , O43318 (MAP3K7) , Q15831 (STK11) HPRD   neXtProt   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P+   RLIMS-P   Signor   UniProt 29480469, 33391485, 22315316, ...31624493, 32681102, 23044421, 18474106, 32393385, 27626315, 22821946, 12145153, 32151973, 26997114, 21778691, 29442133, 22517326, 22359610, 27385336, 29221189, 32398698, 29152112, 25080865, 30011295, 33261193, 23805277, 32377712, 25846006, 32765083, 24767988, 25325265, 31835577, 21562078, 25084564, 30323244, 17083919, 32046043, 20460103, 30061832, 32570982, 33923896, 10642499, 26193886, 21474604, 33008449, 23773688, 17849173, 26861281, 24100028, 29799853, 21062936, 33194631, 16818670, 30467274, 27693630, 22428043, 32356550, 25241895, 26542945, 15980064, 27230293, 22029423, 22492524, 32029897, 32962063, 27633131, 15371448, 31627466, 24434520, 26374855, 27133129, 29654166, 14976552, 21474997, 21673100, 25497784, 22952994, 24823908, 33400924, 24972246, 32639656, 31641367, 26489410, 26804254, 32511787, 14985505, 18693249, 29399091, 23216827, 31515527, 26999735, 33477536, 29755474, 25537507, 28994003, 28611773, 24691558, 20217115, 23942093, 32916992, 31372798, 31001279, 20538596, 33015052, 33543028, 27887844, 24425763, 31404503, 20112400, 33040052, 31763031, 26952277, 29914450, 28974016, 33097833, 22514710, 25849721, 23741428, 19958286, 23935921, 29491475, 33411680, 33819291, 31024752, 32273511, 14614828, 32590330, 33139797, 32244181, 21507243, 22719961, 24746562, 17178807, 24511687, 30018557, 33391522, 33898950, 22451268, 16600998, 23747367, 22284532, 22975059, 33219302, 30864681, 23238131, 30129983, 21673972, 32601620, 33613155, 19666001, 33241200, 24450630, 22291597, 24387300, 33919842, 31569635, 32195105, 19036825, 18691976, 21318153, 25409232, 33766052, 24467442, 12764152, 24389872, 31817196, 20363874, 25061501, 23298764, 26893937, 22574218, 17339833, 27494895, 31541080, 33916175, 14693511, 33207837, 26742424, 23967345, 27809416, 32020870, 32766475, 32152146, 29988028, 30720062, 32083006, 33184378, 31969827, 18614941, 33953705, 22972179
S173 Phosphorylation P17612 (PRKACA) phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor 18669648, 31798532, 19942859, ...18220336
C174 S-Nitrosylation dbSNO 26338901
S176 Phosphorylation HPRD   phospho.ELM   PhosphoSitePlus 18691976, 18220336
Y179 Phosphorylation PhosphoSitePlus
R227 Methylation PhosphoSitePlus
Y232 Phosphorylation PhosphoSitePlus
T243 Phosphorylation RLIMS-P 32937443
K260 Ubiquitination PhosphoSitePlus
K269 Ubiquitination PhosphoSitePlus
Y324 Phosphorylation HPRD   PhosphoSitePlus 20068231
S345 Phosphorylation RLIMS-P 33898950
S377 Phosphorylation HPRD   phospho.ELM   PhosphoSitePlus   RLIMS-P   UniProt 19413330, 18691976, 23186163, ...19369195, 33898950, 18669648, 19415658
N388 N-Glycosylation GlyGen 29741879, 29671580
K391 Ubiquitination PhosphoSitePlus
K401 Sumoylation PhosphoSitePlus
K401 Ubiquitination PhosphoSitePlus
Y436 Phosphorylation PhosphoSitePlus   RLIMS-P 27626315
S481 Phosphorylation PhosphoSitePlus
S483 Phosphorylation PhosphoSitePlus
S484 Phosphorylation PhosphoSitePlus
T485 Phosphorylation Q15831 (STK11) neXtProt   PhosphoSitePlus   RLIMS-P 33898950, 12764152
S489 Phosphorylation PhosphoSitePlus
S491 Phosphorylation P31749 (AKT1) neXtProt   PhosphoSitePlus   RLIMS-P 16340011, 24467442, 26797128, ...27784766
S500 Phosphorylation HPRD   PhosphoSitePlus 18691976
S501 Phosphorylation PhosphoSitePlus
S509 Phosphorylation PhosphoSitePlus
S511 Phosphorylation PhosphoSitePlus
S515 Phosphorylation PhosphoSitePlus
T521 Phosphorylation PhosphoSitePlus
T524 Phosphorylation PhosphoSitePlus
S527 Phosphorylation PhosphoSitePlus
S534 Phosphorylation PhosphoSitePlus


P54646 (PRKAA2) as PTM Enzyme



Substrate Site Score Source PMID
F1D8S2 (NR2A1) S313 HPRD 12740371
O00418 (EEF2K) S78 HPRD 14709557, 15024086
O00418 (EEF2K) S366 HPRD 11500364, 14709557, 15024086, ...11171059
O00418 (EEF2K) S398 HPRD   PhosphoSitePlus 23182879, 14709557, 15024086
O00763-1 (ACACB) S222 HPRD   Signor 20166139, 12413941, 14613924, ...9148944, 14532170
O14920 (IKBKB) S177 neXtProt   PhosphoSitePlus   Signor 21673972
O14920 (IKBKB) S181 neXtProt   PhosphoSitePlus   Signor 21673972
O15151 (MDM4) S342 RLIMS-P+ 24190973
O43524 (FOXO3) T179 PhosphoSitePlus 22137581
O43524 (FOXO3) S399 PhosphoSitePlus 22137581
O43524 (FOXO3) S413 neXtProt   PhosphoSitePlus 17711846, 22137581
O43524 (FOXO3) S555 PhosphoSitePlus 22137581
O43524 (FOXO3) S588 neXtProt   PhosphoSitePlus 17711846, 22137581
O43524 (FOXO3) S626 PhosphoSitePlus 22137581
O60825 (PFKFB2) S466 HPRD   neXtProt   Signor 19413330, 11069105, 18767875, ...19651622, 18669648, 12853467, 20068231, 17924679
O75385 (ULK1) S317 Signor 19584320, 21205641
O75385 (ULK1) S556 Signor 19584320, 21205641
O75385 (ULK1) S638 Signor 19584320, 21205641
P05549 (TFAP2A) S219 PhosphoSitePlus 22561688
P06241 (FYN) T12 neXtProt 20558612
P08151 (GLI1) S102 PhosphoSitePlus 26190112
P08151 (GLI1) S408 PhosphoSitePlus 26190112
P08151 (GLI1) T1074 PhosphoSitePlus 26190112
P09874 (PARP1) S177 PhosphoSitePlus 23382195
P28562 (DUSP1) S334 PhosphoSitePlus 25799226
P29474 (NOS3) S633 neXtProt 19131647
P29474 (NOS3) S1177 neXtProt 19131647
P30260 (CDC27) S379 PhosphoSitePlus   Signor 22137581
P35222 (CTNNB1) S552 neXtProt 20361929
P36956 (SREBF1) S396 Signor 21459323
P41235-3 (HNF4A) S313 HPRD 12740371
P49815 (TSC2) S1387 Signor 16959574
P50552 (VASP) T278 Signor 17082196
P50552 (VASP) S322 Signor 21945940
P55011 (SLC12A2) S77 RLIMS-P+ 20442269
P55011 (SLC12A2) S242 RLIMS-P+ 20442269
Q13085-2 (ACACA) S22 HPRD 2900138, 18212344, 18767875, ...19651622, 18669648, 18707149, 20166139, 11052978, 20068231
Q13085 (ACACA) S78 neXtProt 17519230
Q13085-1 (ACACA) S80 HPRD   PhosphoSitePlus   Signor 2900138, 12015362, 18212344, ...18767875, 15371448, 19651622, 18669648, 18707149, 20166139, 7907095, 20068231
Q13085-4 (ACACA) S117 HPRD 2900138, 18212344, 18767875, ...19651622, 18669648, 18707149, 20166139, 11052978, 20068231
Q13177 (PAK2) S20 PhosphoSitePlus   Signor 22137581
Q13393 (PLD1) S505 PhosphoSitePlus   Signor 20231899
Q15121 (PEA15) S116 RLIMS-P+ 25096718
Q53ET0 (CRTC2) S171 Signor 16148943, 16308421, 20577053
Q86TI0 (TBC1D1) S237 neXtProt 20837646
Q8IY63 (AMOTL1) S793 RLIMS-P+ 25373897
Q8NFG4 (FLCN) S62 RLIMS-P+ 19914239
Q92819 (HAS2) T110 PhosphoSitePlus 21228273
Q96EB6 (SIRT1) T344 RLIMS-P+ 24959379, 22728651
Q9BZL4 (PPP1R12C) S452 PhosphoSitePlus   Signor 22137581
Q9UQB8 (BAIAP2) S366 PhosphoSitePlus   Signor 22137581, 19933840
Q9UQL6 (HDAC5) S259 PhosphoSitePlus 18184930
Q9UQL6 (HDAC5) S498 PhosphoSitePlus 18184930
Q9Y2I7 (PIKFYVE) S307 RLIMS-P+ 24070423


Proteoforms

PRO ID (Short Label) Sites PTM Enzyme Source PMID
PR:000048902 (hPRKAA2/Phos:1) pT172 PRO


PTM-dependent PPI

PTM type Substrate Site Interactant Association type Source PMID
Phosphorylation O70405 (Ulk1) S757 P54646 (PRKAA2) inhibited_association eFIP 33562139
Phosphorylation O75385 (ULK1) S758 P54646 (PRKAA2) unknown eFIP 21383122
Phosphorylation P35222 (CTNNB1) S552 P54646 (PRKAA2) unknown eFIP 30854043
Phosphorylation Q14457 (BECN1) S93 P54646 (PRKAA2) unknown eFIP 25356873
Phosphorylation Q8NFG4 (FLCN) S302 P54646 (PRKAA2) increased_association eFIP 33981707


PTM sites affected in variants

Site Variant Source PMID Disease [Sample source]
K45 R45 Biomuta DOID:363 / uterine cancer [ cosmic, icgc, tcga ]
S165 L165 Biomuta DOID:1324 / lung cancer [ cosmic, tcga ]
Y324 H324 Biomuta DOID:1324 / lung cancer [ cosmic ]
S377 I377 Biomuta DOID:363 / uterine cancer [ cosmic, icgc, tcga ]
S377 N377 Biomuta DOID:1909 / melanoma [ icgc, tcga ]
K391 R391 Biomuta DOID:10534 / stomach cancer [ tcga ]
S484 P484 Biomuta DOID:2394 / ovarian cancer [ icgc ] DOID:2994 / germ cell cancer [ cosmic ]
S501 C501 Biomuta DOID:10534 / stomach cancer [ cosmic, icgc, tcga ]
S515 F515 Biomuta DOID:9256 / colorectal cancer [ tcga ]