Protein Information

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UniProt AC / UniProt ID P45983 / MK08_HUMAN
Protein Name Mitogen-activated protein kinase 8
Gene Name Name: MAPK8
Synonyms:JNK1,PRKM8,SAPK1,SAPK1C;
Organism Homo sapiens (Human)

PRO ID PR:P45983
DAG View
PRO Name mitogen-activated protein kinase 8 (human)
Definition A mitogen-activated protein kinase 8 that is encoded in the genome of human.
Short Label hMAPK8
Category organism-gene

Interactive Sequence View

Select/align proteoforms across species


P45983 (MAPK8) as Substrate



Site PTM Type PTM Enzyme Score Source PMID
S2 Phosphorylation RLIMS-P 24567356
C116 S-Nitrosylation dbSNO 11121042, 22178444, 15688001
S129 Phosphorylation Q05655 (PRKCD) neXtProt   RLIMS-P 23056407, 16483929
S144 Phosphorylation PhosphoSitePlus
K153 Ubiquitination PhosphoSitePlus
S155 Phosphorylation PhosphoSitePlus
K166 Ubiquitination PhosphoSitePlus
T178 Phosphorylation PhosphoSitePlus
S179 Phosphorylation PhosphoSitePlus
T183 Phosphorylation P45985 (MAP2K4) , O14733 (MAP2K7) , Q99683 (MAP3K5) , O95382 (MAP3K6) neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 17192257, 27698876, 20649571, ...27590675, 10506143, 11390361, 9625770, 26987398, 7839144, 9724739, 22216254, 27221050, 17567906, 17205142, 27732954, 15755722, 15911620, 23056407, 23157661, 24533778, 15389828, 18436531, 20581839, 18436711, 14612908, 24244431, 11971971, 19749799, 19258436, 19806201, 19732446, 11062067, 23559002, 27602114, 23566222, 28176854, 9207092, 9312068, 12221076, 18669648, 17210579, 27367023, 18286207, 21040800, 15138488
Y185 Phosphorylation P45985 (MAP2K4) , P07949 (RET) , Q99683 (MAP3K5) , O14733 (MAP2K7) , O95382 (MAP3K6) neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 17192257, 27698876, 20649571, ...27590675, 10506143, 11390361, 9625770, 26987398, 7839144, 9724739, 22216254, 27221050, 17567906, 17205142, 27732954, 15755722, 15911620, 23056407, 23157661, 24533778, 15389828, 18436531, 20581839, 14579416, 18436711, 14612908, 24244431, 11971971, 19749799, 19258436, 19806201, 19732446, 11062067, 23559002, 27602114, 23566222, 28176854, 9207092, 9312068, 18083107, 12221076, 18669648, 16153436, 27367023, 18286207, 21040800, 17210579, 15138488
T188 Phosphorylation PhosphoSitePlus
T243 Phosphorylation PhosphoSitePlus
T255 Phosphorylation PhosphoSitePlus
T258 Phosphorylation PhosphoSitePlus
Y259 Phosphorylation PhosphoSitePlus
S292 Phosphorylation PhosphoSitePlus
K300 Ubiquitination PhosphoSitePlus
S307 Phosphorylation RLIMS-P 25966993, 27034691, 22046423, ...17095576, 23566306
K308 Acetylation PhosphoSitePlus
K308 Ubiquitination PhosphoSitePlus
Y357 Phosphorylation PhosphoSitePlus
T367 Phosphorylation PhosphoSitePlus
S377 Phosphorylation phospho.ELM 18669648
Site PTM Type PTM Enzyme Score Source PMID
T183 Phosphorylation O14733 (MAP2K7) HPRD 18691976, 18669648, 11062067
Y185 Phosphorylation P45985 (MAP2K4) , P07949 (RET) HPRD 18669648, 16153436
T255 Phosphorylation HPRD 17924679
T258 Phosphorylation HPRD 17924679
Y259 Phosphorylation HPRD 17924679
Y357 Phosphorylation HPRD 17924679
T367 Phosphorylation HPRD 17924679
S377 Phosphorylation HPRD 15345747
Site PTM Type PTM Enzyme Score Source PMID
T183 Phosphorylation O14733 (MAP2K7) phospho.ELM   PhosphoSitePlus 18669648, 11062067
Y185 Phosphorylation P45985 (MAP2K4) phospho.ELM   PhosphoSitePlus 18669648, 11062067
K308 Acetylation PhosphoSitePlus
S377 Phosphorylation PhosphoSitePlus
Site PTM Type PTM Enzyme Score Source PMID
T183 Phosphorylation O14733 (MAP2K7) , P45985 (MAP2K4) HPRD   PhosphoSitePlus 18691976, 18669648, 23416355, ...11062067
Y185 Phosphorylation P07949 (RET) , P45985 (MAP2K4) HPRD   PhosphoSitePlus 18669648, 16153436, 23416355
T228 Phosphorylation P45985 (MAP2K4) PhosphoSitePlus 23416355
T255 Phosphorylation HPRD 17924679
T258 Phosphorylation HPRD 17924679
Y259 Phosphorylation HPRD 17924679
S284 Phosphorylation P45985 (MAP2K4) PhosphoSitePlus 23416355
Y357 Phosphorylation HPRD 17924679
T367 Phosphorylation HPRD 17924679
S377 Phosphorylation P45985 (MAP2K4) HPRD   PhosphoSitePlus 19651622, 18669648, 23416355, ...18691976


P45983 (MAPK8) as PTM Enzyme



Substrate Site Score Source PMID
O00418 (EEF2K) S396 HPRD 12171600
O43521-2 (BCL2L11) S44 phospho.ELM   PhosphoSitePlus 12591950
O43521-17 (BCL2L11) T56 HPRD   PhosphoSitePlus 12591950
O43521-2 (BCL2L11) S58 HPRD   PhosphoSitePlus 12591950
O43521-1 (BCL2L11) S59 HPRD 12818176
O43521-1 (BCL2L11) S69 HPRD   neXtProt 12818176, 12591950
O43521-1 (BCL2L11) S77 HPRD 18691976, 12818176
O43521-1 (BCL2L11) S104 HPRD   neXtProt 12591950, 12818176
O43521 (BCL2L11) T116 HPRD   neXtProt   Signor 12591950, 12818176
O43521-1 (BCL2L11) S118 HPRD   neXtProt 12591950, 12818176
O43524 (FOXO3) S574 PhosphoSitePlus 23857333
O43561-2 (LAT) T155 PhosphoSitePlus 15192708
O43561 (LAT) T184 neXtProt   Signor 15192708
O95644 (NFATC1) S172 PhosphoSitePlus 24863062
P01106 (MYC) T58 neXtProt 10551811
P01106 (MYC) S62 neXtProt   PhosphoSitePlus 10551811
P01106 (MYC) S71 neXtProt   PhosphoSitePlus 10551811
P01106-2 (MYC) S77 HPRD 10551811, 19651622, 18669648, ...1748630, 20068231
P01106-2 (MYC) S86 HPRD 10551811, 18767875, 18669648
P04150 (NR3C1) S226 HPRD   neXtProt   PhosphoSitePlus   Signor 18669648, 9199329, 18337589, ...19812390, 12351702
P04637 (TP53) S20 PhosphoSitePlus   Signor 11896587
P04637 (TP53) S37 neXtProt 9393873
P04637-1 (TP53) T81 HPRD   PhosphoSitePlus   Signor 11283254, 12531896
P05067-4 (APP) T668 PhosphoSitePlus 14970211
P05067 (APP) T743 neXtProt   Signor 12917434, 24610780, 14970211
P05412 (JUN) S63 HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   UniProt 19413330, 8224842, 9687508, ...18707149, 18212344, 16009721, 14617628, 21212517, 20058876, 8137421, 19664995, 18452278, 18669648, 11948398, 17671211, 8654373, 11896587, 12900420, 19118012, 20068231, 7737130
P05412 (JUN) S73 HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   UniProt 8224842, 12531896, 16009721, ...14739463, 19664995, 8137421, 12740383, 18669648, 18707149, 22327296, 9687508, 19118012, 7737130
P05412 (JUN) T91 PhosphoSitePlus 17920329
P05412 (JUN) T93 PhosphoSitePlus 17920329
P05787 (KRT8) S74 HPRD   phospho.ELM   PhosphoSitePlus   Signor 9211903, 18691976, 19007248, ...11781324, 11788583
P05787 (KRT8) S432 PhosphoSitePlus 21840417
P08047 (SP1) T278 neXtProt   PhosphoSitePlus   Signor   UniProt 18199680, 19245816
P08047 (SP1) T739 neXtProt   PhosphoSitePlus   Signor   UniProt 18199680, 19245816, 11904305
P10275 (AR) S651 PhosphoSitePlus 16282370
P10276 (RARA) T181 neXtProt   PhosphoSitePlus 15657432
P10276 (RARA) S445 neXtProt   PhosphoSitePlus 15657432
P10276 (RARA) S461 neXtProt   PhosphoSitePlus 15657432
P10415-1 (BCL2) T69 HPRD   PhosphoSitePlus   Signor   UniProt 18570871, 19029119, 10567572
P10415 (BCL2) S70 HPRD   phospho.ELM   PhosphoSitePlus   Signor   UniProt 18570871, 19029119, 10567572, ...12039864
P10415 (BCL2) S87 HPRD   phospho.ELM   PhosphoSitePlus   Signor   UniProt 15225643, 23272207, 12039864, ...19029119, 10567572, 18570871
P10636-8 (MAPT) T181 PhosphoSitePlus 12801934
P10636-8 (MAPT) S202 PhosphoSitePlus 12801934
P10636-8 (MAPT) T231 PhosphoSitePlus 12801934
P10636-8 (MAPT) S396 PhosphoSitePlus 16478768
P10636 (MAPT) T498 neXtProt 15228592
P10636 (MAPT) S516 neXtProt 15228592
P10636 (MAPT) S519 neXtProt 15228592
P10636 (MAPT) T522 neXtProt 15228592
P10636 (MAPT) T529 neXtProt 15228592
P10636 (MAPT) T534 neXtProt 15228592
P10636 (MAPT) S713 neXtProt 15228592
P10636 (MAPT) S721 neXtProt 15228592
P10636 (MAPT) S739 neXtProt 15228592
P14618-2 (PKM) T365 PhosphoSitePlus 26258887
P15336 (ATF2) T69 PhosphoSitePlus   Signor 15304344, 10777545, 11078583, ...7737129, 12110590, 7824938, 7737130
P15336 (ATF2) T71 PhosphoSitePlus   Signor 15304344, 10777545, 11078583, ...7737129, 12110590, 7824938, 7737130
P15336 (ATF2) S90 PhosphoSitePlus 11078583, 15304344, 7737130
P16104 (H2AFX) S140 neXtProt   Signor 19234442, 16818236
P16949 (STMN1) S25 neXtProt 20630875
P16949 (STMN1) S38 neXtProt   Signor 20630875
P17275 (JUNB) T102 HPRD   neXtProt   PhosphoSitePlus 18669648, 9889198, 20068231, ...8917518
P17275 (JUNB) T104 HPRD   neXtProt   PhosphoSitePlus 18669648, 9889198, 20068231, ...8917518
P17535 (JUND) S90 neXtProt 12052834
P17535 (JUND) S100 neXtProt   PhosphoSitePlus   Signor 22327296, 12052834
P17535 (JUND) T117 neXtProt 12052834
P19419 (ELK1) S383 Signor   UniProt 8846788, 10637505
P19419 (ELK1) S389 Signor 7651411, 8846788
P19793 (RXRA) S56 neXtProt 10383391
P19793 (RXRA) S70 neXtProt 10383391
P19793 (RXRA) T82 neXtProt 10383391
P19793 (RXRA) S260 neXtProt   PhosphoSitePlus   Signor 16551633, 10383391
P22736 (NR4A1) S95 neXtProt   PhosphoSitePlus   Signor 17023523
P23443 (RPS6KB1) S434 neXtProt 11279232
P29353 (SHC1) S36 neXtProt 11602589
P30305-2 (CDC25B) S101 PhosphoSitePlus 19638579
P30305-2 (CDC25B) S103 PhosphoSitePlus 21807946, 19638579
P30305 (CDC25B) S115 neXtProt 19638579
P30305 (CDC25B) S117 neXtProt 19638579
P30307 (CDC25C) S168 neXtProt   PhosphoSitePlus   Signor 20220133
P31946 (YWHAB) S186 neXtProt   phospho.ELM   PhosphoSitePlus   Signor 15696159
P31947 (SFN) S186 neXtProt   phospho.ELM   PhosphoSitePlus   Signor 15071501
P35222 (CTNNB1) S33 PhosphoSitePlus 19667122
P35222 (CTNNB1) S37 neXtProt   PhosphoSitePlus 19667122
P35222 (CTNNB1) T41 neXtProt   PhosphoSitePlus 19667122
P35568 (IRS1) S307 Signor 12510059
P35568 (IRS1) S312 HPRD   neXtProt   PhosphoSitePlus   Signor   UniProt 12351658, 11606564, 15001544, ...18498745, 10722755, 18728222
P35568 (IRS1) S315 Signor 12510059
P35568 (IRS1) S616 Signor 12510059
P35568 (IRS1) S636 Signor 12510059
P36956-3 (SREBF1) T81 PhosphoSitePlus 25353341
P36956-3 (SREBF1) S93 PhosphoSitePlus 25353341
P36956 (SREBF1) S117 PhosphoSitePlus 22384276
P37231-2 (PPARG) S84 HPRD 9030579
P37231 (PPARG) S112 HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   Signor 9030579
P38936 (CDKN1A) T57 HPRD   neXtProt   PhosphoSitePlus 12058028, 19822666
P38936 (CDKN1A) S130 HPRD   neXtProt   PhosphoSitePlus   Signor 12058028, 19664994, 18669648, ...16964243
P40763-2 (STAT3) S726 HPRD 19413330, 18691976, 12763138, ...9343414, 12576423, 20058876, 11350938, 9872331, 10521505, 18669648, 18491316, 14551213, 20068231
P40763 (STAT3) S727 HPRD   neXtProt   PhosphoSitePlus   Signor 19413330, 18691976, 12763138, ...10446219, 12576423, 20058876, 11350938, 9872331, 10521505, 18669648, 18491316, 20068231, 14551213, 23255093, 12562765, 9343414
P41970 (ELK3) S357 HPRD   neXtProt 11042694
P41970 (ELK3) S363 neXtProt 11042694
P41970 (ELK3) S396 neXtProt 11042694
P41970 (ELK3) S401 neXtProt 11042694
P42224 (STAT1) S727 neXtProt 14963018
P42226 (STAT6) S707 neXtProt   PhosphoSitePlus   Signor 21123173
P46937 (YAP1) T119 UniProt 20068231
P46937 (YAP1) S138 UniProt 18669648
P46937 (YAP1) T154 UniProt 18669648
P46937 (YAP1) S367 UniProt 18669648
P46937 (YAP1) T412 UniProt 21364637
P49023 (PXN) S178 HPRD   neXtProt   PhosphoSitePlus 12853963, 20068231
P54259 (ATN1) S739 PhosphoSitePlus   Signor   UniProt 12812981
P55957 (BID) T59 neXtProt   PhosphoSitePlus 25077544
P61978 (HNRNPK) S216 PhosphoSitePlus   Signor 11259409
P61978 (HNRNPK) S353 phospho.ELM   PhosphoSitePlus   Signor 11259409
P63104-1 (YWHAZ) S184 HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   Signor   UniProt 15696159, 15071501, 16009721
P68431 (HIST1H3J) S29 neXtProt 11278789
P78352 (DLG4) S295 neXtProt 17988632
P98177 (FOXO4) T227 neXtProt   PhosphoSitePlus 20959475
P98177 (FOXO4) S230 neXtProt   PhosphoSitePlus 20959475
P98177 (FOXO4) T451 neXtProt   phospho.ELM   PhosphoSitePlus   Signor 15538382, 20959475
P98177 (FOXO4) T455 neXtProt   phospho.ELM   PhosphoSitePlus   Signor 15538382, 20959475
Q00613-1 (HSF1) S363 HPRD   neXtProt   Signor   UniProt 18767875, 10747973, 19651622, ...18669648, 20166139, 20068231
Q01844 (EWSR1) T79 neXtProt   PhosphoSitePlus 19076070
Q05639 (EEF1A2) S205 PhosphoSitePlus 23608534
Q05639 (EEF1A2) S358 PhosphoSitePlus 23608534
Q06481 (APLP2) T736 neXtProt   phospho.ELM   PhosphoSitePlus   Signor 14970211
Q07817 (BCL2L1) T47 phospho.ELM   PhosphoSitePlus 10617621
Q07817 (BCL2L1) S62 phospho.ELM   PhosphoSitePlus   Signor 22617334, 12633850
Q07817 (BCL2L1) T115 phospho.ELM   PhosphoSitePlus 10617621
Q07820 (MCL1) S64 neXtProt   PhosphoSitePlus 17463001
Q07820 (MCL1) S121 PhosphoSitePlus 12223490
Q07820 (MCL1) T163 PhosphoSitePlus   Signor 18676833, 12223490
Q12904 (AIMP1) S140 PhosphoSitePlus   Signor 20510162
Q12904 (AIMP1) T164 neXtProt 20510162
Q13043 (STK4) S82 neXtProt   PhosphoSitePlus   Signor 20028971
Q13363 (CTBP1) S422 neXtProt   PhosphoSitePlus   Signor 16984892
Q13950 (RUNX2) S118 PhosphoSitePlus 22247071
Q14653 (IRF3) S173 neXtProt   PhosphoSitePlus   Signor 19153595
Q14934 (NFATC4) S213 neXtProt   PhosphoSitePlus   UniProt 17875713
Q14934 (NFATC4) S217 neXtProt   PhosphoSitePlus   UniProt 17875713
Q15672 (TWIST1) S68 neXtProt   PhosphoSitePlus   Signor 21502402
Q16621 (NFE2) S157 PhosphoSitePlus 19966288
Q5JR12 (PPM1J) S93 neXtProt   Signor 18553930
Q5JR12 (PPM1J) T201 neXtProt 18553930
Q5JR12 (PPM1J) T204 neXtProt 18553930
Q6JBY9 (RCSD1) S68 UniProt 23186163
Q6JBY9 (RCSD1) S83 UniProt 23186163
Q6JBY9 (RCSD1) S216 UniProt 19369195
Q8N122 (RPTOR) S696 PhosphoSitePlus   UniProt 22493283, 23186163
Q8N122 (RPTOR) T706 PhosphoSitePlus   UniProt 22493283, 19864431
Q8N122 (RPTOR) S863 PhosphoSitePlus   UniProt 22493283, 16964243
Q8WYK2-1 (JDP2) T148 HPRD   neXtProt   UniProt 12225289, 11602244, 23186163
Q92934 (BAD) S75 neXtProt 11983683, 16166315
Q92934 (BAD) S91 neXtProt 11983683, 16166315
Q93045 (STMN2) S62 neXtProt 16618812
Q93045 (STMN2) S73 neXtProt 16618812
Q96EB6 (SIRT1) S27 neXtProt   PhosphoSitePlus   Signor   UniProt 23186163, 20027304
Q96EB6 (SIRT1) S47 neXtProt   PhosphoSitePlus   Signor   UniProt 21540183, 16964243, 20027304
Q96EB6 (SIRT1) T530 neXtProt   PhosphoSitePlus   Signor   UniProt 19690332, 20027304
Q96J02 (ITCH) S240 neXtProt   Signor 16446428
Q96J02 (ITCH) T263 neXtProt   Signor 16446428
Q96J02 (ITCH) S273 neXtProt   Signor 16446428
Q96LC9-2 (BMF) S74 HPRD   PhosphoSitePlus 12591950
Q96LC9-2 (BMF) S77 HPRD 12591950
Q99607 (ELF4) S641 PhosphoSitePlus 21465527
Q99640 (PKMYT1) S83 neXtProt 19204086
Q9H211 (CDT1) T29 neXtProt   PhosphoSitePlus   UniProt 21856198
Q9H211 (CDT1) S93 UniProt 21856198
Q9H211 (CDT1) S318 UniProt 19690332
Q9H2B2 (SYT4) S135 PhosphoSitePlus 18046461
Q9NPI6 (DCP1A) S315 PhosphoSitePlus 21859862
Q9NR28 (DIABLO) S6 PhosphoSitePlus 25310587
Q9NR28 (DIABLO) S9 PhosphoSitePlus 25310587
Q9NRA8 (EIF4ENIF1) S301 PhosphoSitePlus   Signor 22966201
Q9NRA8 (EIF4ENIF1) S374 PhosphoSitePlus   Signor 22966201
Q9NRA8 (EIF4ENIF1) S513 PhosphoSitePlus   Signor 22966201
Q9NRA8 (EIF4ENIF1) S587 PhosphoSitePlus   Signor 22966201
Q9NRA8 (EIF4ENIF1) S693 PhosphoSitePlus   Signor 22966201
Q9NRA8 (EIF4ENIF1) S752 PhosphoSitePlus   Signor 22966201
Q9UPT6-1 (MAPK8IP3) T265 HPRD   neXtProt   Signor 15767678, 10523642, 10629060
Q9UPT6-1 (MAPK8IP3) T275 HPRD   neXtProt   Signor 15767678, 10523642, 10629060
Q9UPT6 (MAPK8IP3) T286 HPRD   neXtProt   Signor 15767678, 10523642, 10629060
Q9UQF2 (MAPK8IP1) S15 HPRD 12756254
Q9UQF2 (MAPK8IP1) S29 HPRD 12756254
Q9UQF2 (MAPK8IP1) T103 HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   Signor   UniProt 12756254
Q9UQF2 (MAPK8IP1) S197 HPRD 12756254
Q9UQF2 (MAPK8IP1) T205 HPRD   neXtProt   PhosphoSitePlus   UniProt 12756254
Q9UQF2 (MAPK8IP1) T284 HPRD 12756254
Q9UQF2 (MAPK8IP1) S341 HPRD 12756254
Q9UQF2 (MAPK8IP1) S421 HPRD 12756254
Q9Y4H2 (IRS2) T350 neXtProt 17050683
Q9Y4H2 (IRS2) S491 neXtProt 18029441
Q9Y5Q3 (MAFB) T62 neXtProt 19932079
Q9Y6Q9 (NCOA3) T24 neXtProt   PhosphoSitePlus 15383283
Q9Y6Q9 (NCOA3) S505 neXtProt   PhosphoSitePlus 15383283
Q9Y6Q9 (NCOA3) S543 neXtProt   PhosphoSitePlus 15383283
Q9Y6Q9 (NCOA3) S860 neXtProt   PhosphoSitePlus 15383283
Q9Y6Q9 (NCOA3) S867 neXtProt   PhosphoSitePlus 15383283


PTM-dependent PPI

PTM type Substrate Site Interactant Association type Source PMID
Phosphorylation P31749 (AKT1) S473 P45983 (MAPK8) unknown eFIP 22771387
Phosphorylation Q9NZC7 (WWOX) Y33 P45983 (MAPK8) unknown eFIP 14555208


Proteoform PPIs

Protein 1 Relation Protein 2 Source PMID
PR:O95644-1 (hNFATC1/iso:C-alpha) Interaction PR:P45983 (hMAPK8) PRO 10866678