iPTMnet Report for P29474 (NOS3)


Protein Information

Cytoscape View


UniProt AC / UniProt ID P29474 / NOS3_HUMAN
Protein Name Nitric oxide synthase 3
Gene Name Name: NOS3
Organism Homo sapiens (Human)

PRO ID PR:P29474
DAG View
PRO Name nitric oxide synthase, endothelial (human)
Definition A nitric oxide synthase, endothelial that is encoded in the genome of human.
Short Label hNOS3
Category organism-gene

Interactive Sequence View

Select/align proteoforms across species


P29474 (NOS3) as Substrate



Site PTM Type PTM Enzyme Score Source PMID
K5 Methylation PhosphoSitePlus
T33 Phosphorylation PhosphoSitePlus   UniProt 24275569
S53 Phosphorylation PhosphoSitePlus
S78 O-Glycosylation GlyGen 30459171
Y81 Phosphorylation phospho.ELM   PhosphoSitePlus   RLIMS-P 18083107, 32653904
C94 S-Nitrosylation dbSNO 15774480, 17941803, 22178444
C99 S-Nitrosylation dbSNO 17941803, 22178444, 16081307
S102 Phosphorylation PhosphoSitePlus
S114 Phosphorylation PhosphoSitePlus   RLIMS-P   Signor   UniProt 23624269, 20213743, 16270749, ...32590330, 23264539
S114 Phosphorylation Q00535 (CDK5) PhosphoSitePlus   Signor 20213743
C184 S-Nitrosylation dbSNO 22178444, 16081307
C201 S-Nitrosylation dbSNO 22178444, 16081307
Y210 Phosphorylation PhosphoSitePlus
C212 S-Nitrosylation dbSNO 22178444, 16081307
S260 Phosphorylation PhosphoSitePlus
T495 Phosphorylation neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 29566307, 32781622, 32863226, ...32705193, 27170438, 19100855, 33899122, 26890696, 35258406, 35884891, 24008236, 35300809, 22542797, 23624269, 15721870, 10671564, 21063103, 17651694, 12482742, 25419657, 18931031, 22469105, 24379783, 23836447, 25407137, 32161546, 20211863, 28870504, 35617030, 28000882, 15710249, 25020117, 16126727, 35074481, 32590330, 18622039, 21147698, 17784906, 27593859, 29410170, 29466710, 33221570, 28559108, 25869503, 28581447, 11397791, 32872351, 36312762, 23804534, 21383022, 23959544, 30048241, 16344378
T495 Phosphorylation O75116 (ROCK2) neXtProt 17651694
T495 Phosphorylation P05129 (PRKCG) Signor 24379783
T495 Phosphorylation P05771 (PRKCB) Signor 24379783
T495 Phosphorylation P17252 (PRKCA) PhosphoSitePlus   Signor 18622039, 24379783
T495 Phosphorylation P17612 (PRKACA) PhosphoSitePlus 10671564
T495 Phosphorylation P24723 (PRKCH) Signor 24379783
T495 Phosphorylation P41743 (PRKCI) Signor 24379783
T495 Phosphorylation Q02156 (PRKCE) Signor 24379783
T495 Phosphorylation Q04759 (PRKCQ) Signor 24379783
T495 Phosphorylation Q05513 (PRKCZ) Signor 24379783
T495 Phosphorylation Q05655 (PRKCD) Signor 24379783
T495 Phosphorylation Q13131 (PRKAA1) Signor 24379783
T495 Phosphorylation Q13237 (PRKG2) PhosphoSitePlus 10671564
S508 Phosphorylation PhosphoSitePlus
T512 Phosphorylation PhosphoSitePlus
S600 Phosphorylation PhosphoSitePlus
S615 O-Glycosylation GlyGen 32863226
S615 Phosphorylation neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor 10671564, 12171920, 32863226, ...24379783
S615 Phosphorylation P17612 (PRKACA) neXtProt   PhosphoSitePlus   Signor 12171920, 10671564, 24379783
S615 Phosphorylation P31749 (AKT1) neXtProt   PhosphoSitePlus   Signor 12171920, 24379783
S615 Phosphorylation P31751 (AKT2) Signor 24379783
S615 Phosphorylation Q9Y243 (AKT3) Signor 24379783
S617 Phosphorylation RLIMS-P 32554303
S633 Phosphorylation neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 10671564, 32781622, 28000882, ...12171920, 32863226, 20299462, 24379783, 19131647, 25170746, 32590330, 24275569, 35182051, 20881236, 32606304, 20479254, 16344378
S633 Phosphorylation P17612 (PRKACA) neXtProt   PhosphoSitePlus   Signor 12171920, 10671564, 24379783
S633 Phosphorylation P54646 (PRKAA2) neXtProt 19131647
S633 Phosphorylation Q13131 (PRKAA1) neXtProt   PhosphoSitePlus 20479254, 19131647
S633 Phosphorylation Q13237 (PRKG2) PhosphoSitePlus 10671564
S634 Phosphorylation PhosphoSitePlus
T636 Phosphorylation PhosphoSitePlus
S638 Phosphorylation PhosphoSitePlus   UniProt 24275569
Y657 Phosphorylation PhosphoSitePlus   Signor 18483407, 19934023
Y657 Phosphorylation Q14289 (PTK2B) PhosphoSitePlus   Signor 18483407, 19934023
C661 S-Nitrosylation dbSNO 17941803, 22178444
S738 Phosphorylation PhosphoSitePlus
K773 Ubiquitination PhosphoSitePlus
C802 S-Nitrosylation dbSNO 17941803, 22178444
K834 Ubiquitination PhosphoSitePlus
S836 Phosphorylation PhosphoSitePlus   UniProt 24275569
C853 S-Nitrosylation dbSNO 17941803, 22178444
T854 Phosphorylation PhosphoSitePlus
T866 O-Glycosylation GlyGen 33168911, 34050207, 32863226
S870 Phosphorylation PhosphoSitePlus
K904 Ubiquitination PhosphoSitePlus
C976 S-Nitrosylation dbSNO 17941803, 22178444
C991 S-Nitrosylation dbSNO 17941803, 22178444
K1035 Ubiquitination PhosphoSitePlus
C1048 S-Nitrosylation dbSNO 17941803, 22178444
C1050 S-Nitrosylation dbSNO 17941803, 22178444
K1085 Ubiquitination PhosphoSitePlus
T1094 Phosphorylation PhosphoSitePlus
C1114 S-Nitrosylation dbSNO 17941803, 22178444
T1175 Phosphorylation PhosphoSitePlus
S1177 O-Glycosylation GlyGen   PhosphoSitePlus 33168911, 34050207, 32863226
S1177 Phosphorylation neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 29566307, 32781622, 34026871, ...31754254, 28912066, 22728282, 32676485, 20479254, 28725180, 19320971, 18303014, 31973180, 22542797, 20375905, 20416296, 10671564, 29632805, 20299462, 11029403, 11934843, 12482742, 17276402, 29888556, 20881236, 27463705, 11826414, 29104511, 28000882, 15710249, 29374229, 11729179, 32927592, 18622039, 17242275, 19881293, 35874500, 28581447, 24804268, 26286023, 11397791, 36312762, 27235860, 25170746, 21757745, 15933265, 30513637, 18551195, 22120969, 28067284, 32863226, 11319202, 24827148, 23264539, 14661033, 15381390, 35300809, 26662566, 16410224, 22294688, 19262472, 33168911, 24376548, 16545819, 15319431, 27773804, 22469105, 27279530, 32544536, 17229561, 12511559, 19131647, 28627664, 12644458, 21741389, 16643490, 21147698, 29466710, 16036314, 25869503, 29899706, 21139021, 27170438, 19073885, 10908731, 32977740, 30048241, 16344378, 27563480, 10376603, 23204109, 15737995, 18062898, 18564176, 33899122, 22323292, 16840783, 23186163, 24914683, 15733940, 21063103, 28801524, 25419657, 28214453, 17016497, 22037549, 33506378, 11701613, 23836447, 12171920, 25133540, 23624269, 16946136, 12446767, 31747921, 18326799, 20108211, 23284736, 28409476, 31744135, 32590330, 35157107, 17784906, 27593859, 24587005, 34954278, 27923677, 18242595, 19342481, 16497987, 32566092, 24971375, 12791703, 17572401, 28870504, 33320446, 24887036, 32251485, 21234858, 11696579, 33129903, 19501907, 32872351, 16043647, 19351806, 32606304, 35217004, 19465546, 35195045, 18931031, 31871538, 24319055, 31584065, 19136580, 25407137, 32161546, 24757494, 30471424, 23328297, 19188511, 16126727, 22219320, 16270749, 19632222, 24520379, 32653904, 16873402, 26297582, 18414637, 23804534, 28559108, 35884891, 23604495, 36305972, 32696742, 14985356, 27002278, 29563255, 32106617, 23959544, 29410170
S1177 Phosphorylation P17612 (PRKACA) neXtProt   PhosphoSitePlus   Signor 12171920, 10671564, 11729179
S1177 Phosphorylation P22612 (PRKACG) Signor 11729179
S1177 Phosphorylation P31749 (AKT1) neXtProt   PhosphoSitePlus   Signor 10376603, 12446767, 12511559, ...11729179, 18622039, 12171920
S1177 Phosphorylation P54646 (PRKAA2) neXtProt 19131647
S1177 Phosphorylation Q13131 (PRKAA1) PhosphoSitePlus   Signor 20479254, 18303014, 17276402, ...12791703
S1177 Phosphorylation Q13237 (PRKG2) neXtProt   PhosphoSitePlus 18551195, 10671564
S1177 Phosphorylation Q9Y478 (PRKAB1) Signor 11729179
S1179 Phosphorylation phospho.ELM   PhosphoSitePlus   RLIMS-P+   RLIMS-P 10376602, 31781259, 12501214, ...18617528, 23796957, 15784500, 15737995, 26304753, 27613870, 12591925, 24928905, 11988487, 22801313, 23148920, 16864653, 25020117, 11729179, 18435486, 16737962, 18242595, 35409341, 21963997, 29059213, 15642349
S1179 Phosphorylation P31749 (AKT1) RLIMS-P+ 11988487
Site PTM Type PTM Enzyme Score Source PMID
Y81 Phosphorylation HPRD 18083107
S633 Phosphorylation HPRD 20166139
T1175 Phosphorylation HPRD 20166139
S1177 Phosphorylation HPRD 20166139, 10376603, 11696579
S1177 Phosphorylation P31749 (AKT1) HPRD 20166139, 10376603, 11696579


PTM-dependent PPI

PTM type Substrate Site Interactant Association type Source PMID
Phosphorylation P29474 (NOS3) S1179 Q9NYG2 (ZDHHC3) unknown eFIP 16864653
Phosphorylation P29474 (NOS3) S1177 P07900 (HSP90AA1) decreased_association eFIP 28870504
Phosphorylation Q03135 (CAV1) Y14 P29474 (NOS3) increased_association eFIP 22323292


PTM sites affected in variants

Site Variant Source PMID Disease [Sample source]
K5 N5 Biomuta DOID:11054 / urinary bladder cancer [ cosmic, icgc ]
S53 G53 Biomuta DOID:10534 / stomach cancer [ cosmic ]
S53 I53 Biomuta [ cosmic ] DOID:1115 / sarcoma [ icgc ]
S102 F102 Biomuta DOID:4159 / skin cancer [ cosmic ]
S600 F600 Biomuta DOID:4159 / skin cancer [ cosmic, icgc ]
S617 F617 Biomuta DOID:4159 / skin cancer [ cosmic, icgc ]
S617 P617 Biomuta DOID:10534 / stomach cancer [ cosmic ]
Y657 C657 Biomuta DOID:1612 / breast cancer [ cosmic, icgc ]
K904 N904 Biomuta DOID:4362 / cervical cancer [ cosmic, icgc ]
C1048 Y1048 Biomuta DOID:10534 / stomach cancer [ cosmic ]