Protein Information

Cytoscape View


UniProt AC / UniProt ID P10398 / ARAF_HUMAN
Protein Name Serine/threonine-protein kinase A-Raf
Gene Name Name: ARAF
Synonyms:ARAF1,PKS,PKS2;
Organism Homo sapiens (Human)

PRO ID PR:P10398
DAG View
PRO Name serine/threonine-protein kinase A-Raf (human)
Definition A serine/threonine-protein kinase A-Raf that is encoded in the genome of human.
Short Label hARAF
Category organism-gene

Interactive Sequence View

Select/align proteoforms across species


P10398 (ARAF) as Substrate



Site PTM Type PTM Enzyme Score Source PMID
S15 Phosphorylation PhosphoSitePlus
T20 Phosphorylation PhosphoSitePlus
Y24 Phosphorylation PhosphoSitePlus
K28 Ubiquitination PhosphoSitePlus
Y42 Phosphorylation PhosphoSitePlus
Y155 Phosphorylation PhosphoSitePlus
S157 Phosphorylation PhosphoSitePlus   UniProt 23186163
S162 Phosphorylation PhosphoSitePlus   UniProt 23186163
T181 Phosphorylation PhosphoSitePlus   UniProt 24275569
S186 Phosphorylation phospho.ELM   PhosphoSitePlus   UniProt 18691976, 17081983, 24275569
S212 Phosphorylation phospho.ELM   PhosphoSitePlus 18669648
T213 Phosphorylation phospho.ELM   PhosphoSitePlus 18220336
S214 Phosphorylation phospho.ELM   PRO   PhosphoSitePlus 18669648
T215 Phosphorylation phospho.ELM   PhosphoSitePlus 18669648, 18220336
S222 Phosphorylation phospho.ELM   PhosphoSitePlus 18669648
T223 Phosphorylation phospho.ELM   PhosphoSitePlus 18669648
T224 Phosphorylation phospho.ELM   PhosphoSitePlus 18669648
S229 Phosphorylation PhosphoSitePlus
S250 Phosphorylation PhosphoSitePlus
T253 Phosphorylation PhosphoSitePlus   UniProt 23186163
S257 Phosphorylation phospho.ELM   PhosphoSitePlus   RLIMS-P   UniProt 23186163, 16964243, 24275569, ...31541179
S259 Phosphorylation PhosphoSitePlus
S262 Phosphorylation PhosphoSitePlus
S264 Phosphorylation PhosphoSitePlus
S265 Phosphorylation PhosphoSitePlus
S269 Phosphorylation PhosphoSitePlus   UniProt 23186163
S272 Phosphorylation PhosphoSitePlus
S274 Phosphorylation PhosphoSitePlus
K282 Ubiquitination PhosphoSitePlus
S283 Phosphorylation PhosphoSitePlus
K292 Ubiquitination PhosphoSitePlus
Y296 Phosphorylation PhosphoSitePlus
S299 Phosphorylation Q13153 (PAK1) phospho.ELM   PRO   PhosphoSitePlus   Signor 15710605
Y301 Phosphorylation P12931 (SRC) phospho.ELM   PhosphoSitePlus 9020159
Y302 Phosphorylation P12931 (SRC) phospho.ELM   PRO   PhosphoSitePlus 9020159
K314 Ubiquitination PhosphoSitePlus
T318 Phosphorylation PhosphoSitePlus   UniProt 19690332
S320 Phosphorylation PhosphoSitePlus
T323 Phosphorylation PhosphoSitePlus
K339 Ubiquitination PhosphoSitePlus
S341 Phosphorylation PhosphoSitePlus
T344 Phosphorylation PhosphoSitePlus
K352 Ubiquitination PhosphoSitePlus
T413 Phosphorylation PhosphoSitePlus
Y419 Phosphorylation PhosphoSitePlus
K423 Ubiquitination PhosphoSitePlus
K431 Ubiquitination PhosphoSitePlus
S432 Phosphorylation PhosphoSitePlus
T442 Phosphorylation PhosphoSitePlus
T452 Phosphorylation PRO   PhosphoSitePlus
K454 Ubiquitination PhosphoSitePlus
T455 Phosphorylation PRO   PhosphoSitePlus
S458 Phosphorylation PhosphoSitePlus
Y526 Phosphorylation PhosphoSitePlus
S528 Phosphorylation PhosphoSitePlus
S532 Phosphorylation PhosphoSitePlus
S535 Phosphorylation PhosphoSitePlus
S536 Phosphorylation PhosphoSitePlus
K540 Ubiquitination PhosphoSitePlus
S547 Phosphorylation PhosphoSitePlus
K551 Ubiquitination PhosphoSitePlus
S573 Phosphorylation PhosphoSitePlus
K576 Ubiquitination PhosphoSitePlus
S580 Phosphorylation PhosphoSitePlus
S582 Phosphorylation phospho.ELM   PRO   PhosphoSitePlus 17081983
S585 Phosphorylation PhosphoSitePlus
T589 Phosphorylation PhosphoSitePlus
S600 Phosphorylation PhosphoSitePlus
Site PTM Type PTM Enzyme Score Source PMID
T181 Phosphorylation HPRD 18691976, 17081983
S186 Phosphorylation HPRD 19651622, 18691976, 17081983, ...20068231
S212 Phosphorylation HPRD 18691976, 18669648, 20068231
S214 Phosphorylation HPRD 18669648, 20058876
T215 Phosphorylation HPRD 18669648, 18491316
S222 Phosphorylation HPRD 18669648
T223 Phosphorylation HPRD 18669648
T224 Phosphorylation HPRD 18669648
T253 Phosphorylation HPRD 18691976
S257 Phosphorylation HPRD 18691976, 16964243, 19691289
S259 Phosphorylation HPRD 18691976
S269 Phosphorylation HPRD 20068231
S272 Phosphorylation HPRD 20068231
S580 Phosphorylation HPRD 19651622, 18691976
S582 Phosphorylation HPRD 18691976, 17081983, 18767875, ...19691289, 19651622, 20166139, 19415658, 18212344


P10398 (ARAF) as PTM Enzyme



Substrate Site Score Source PMID
Q02750 (MAP2K1) S218 HPRD   neXtProt   PhosphoSitePlus   Signor 7731720, 8157000, 8621729, ...8394352
Q02750 (MAP2K1) S222 HPRD   neXtProt   PhosphoSitePlus   Signor 7731720, 8157000, 8621729, ...8394352
Q15599 (SLC9A3R2) S303 PhosphoSitePlus 26310448
Q92934 (BAD) S75 PhosphoSitePlus 19667065
Q92934 (BAD) S99 PhosphoSitePlus 19667065
Q92934 (BAD) S118 PhosphoSitePlus 19667065


Proteoforms

PRO ID (Short Label) Sites PTM Enzyme Source PMID
PR:000046371 (hARAF/Phos:1) pS214,pS582 PRO
PR:000046372 (hARAF/Phos:2) pS299,pY302,pT452,pT455,pS582 PRO
PR:000046373 (hARAF/Phos:3) pS582 PRO


PTM sites affected in variants

Site Variant Source PMID Disease [Sample source]
T213 M213 Biomuta DOID:9256 / colorectal cancer [ icgc, tcga ]
S214 F214 Biomuta DOID:1909 / melanoma [ icgc, tcga ] DOID:1324 / lung cancer [ tcga ]
S214 C214 Biomuta DOID:1324 / lung cancer [ civic, cosmic, tcga ]
S214 T214 Biomuta DOID:1324 / lung cancer [ cosmic, tcga ]
S214 Y214 Biomuta DOID:2531 / hematologic cancer [ icgc ]
T215 P215 Biomuta DOID:1324 / lung cancer [ cosmic ]
S262 I262 Biomuta DOID:2394 / ovarian cancer [ icgc ] DOID:2994 / germ cell cancer [ cosmic ]
S265 L265 Biomuta DOID:363 / uterine cancer [ tcga ]
Y296 C296 Biomuta DOID:363 / uterine cancer [ tcga ]
S299 * Biomuta DOID:4362 / cervical cancer [ cosmic, icgc, tcga ]
T318 M318 Biomuta DOID:363 / uterine cancer [ cosmic, icgc, tcga ]
K431 N431 Biomuta DOID:363 / uterine cancer [ tcga ]
S432 Y432 Biomuta DOID:363 / uterine cancer [ tcga ]
S432 P432 Biomuta DOID:363 / uterine cancer [ cosmic, icgc, tcga ]
K576 N576 Biomuta DOID:3963 / thyroid carcinoma [ cosmic ]