Protein Information

Cytoscape View


UniProt AC / UniProt ID P06400 / RB_HUMAN
Protein Name Retinoblastoma-associated protein
Gene Name Name: RB1
Organism Homo sapiens (Human)

PRO ID PR:P06400
DAG View
PRO Name retinoblastoma-associated protein (human)
Definition A retinoblastoma-associated protein that is encoded in the genome of human.
Short Label hRB1
Category organism-gene

Interactive Sequence View

Select/align proteoforms across species


P06400 (RB1) as Substrate



Site PTM Type PTM Enzyme Score Source PMID
Phosphorylation PRO
T5 Phosphorylation P11802 (CDK4) HPRD   neXtProt   PhosphoSitePlus 9139732, 22094256
S37 Phosphorylation HPRD   phospho.ELM   PhosphoSitePlus   UniProt 19415658, 18669648, 24275569, ...19690332, 20068231
K63 Ubiquitination PhosphoSitePlus
K65 Ubiquitination PhosphoSitePlus
K94 Ubiquitination PhosphoSitePlus
K136 Ubiquitination PhosphoSitePlus
T140 Phosphorylation PhosphoSitePlus
K143 Ubiquitination PhosphoSitePlus
S149 Phosphorylation PhosphoSitePlus
S163 Phosphorylation PhosphoSitePlus
S230 Phosphorylation P24941 (CDK2) HPRD   PhosphoSitePlus 10207050
Y239 Phosphorylation PhosphoSitePlus
T241 Phosphorylation PhosphoSitePlus
S249 Phosphorylation P24941 (CDK2) , P06493 (CDK1) , P11802 (CDK4) , P05771 (PRKCB) HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 21368870, 22279936, 9139732, ...21406692, 23186163, 15809340, 29468545, 1756735, 23304241, 19690332, 10499802, 18220336, 27419601, 20058876, 24815188, 19651622, 15355984, 22094256, 19664994, 19664995, 18669648, 11805327, 9190208, 8626527, 20068231
T252 Phosphorylation P06493 (CDK1) , P11802 (CDK4) , P05771 (PRKCB) , P24941 (CDK2) HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 22094256, 10499802, 23186163, ...18220336, 27419601, 21368870, 1756735, 24815188, 22279936, 18669648, 11805327, 23304241, 15355984, 9139732, 9190208, 8626527, 20068231
K265 Ubiquitination PhosphoSitePlus
K279 Ubiquitination PhosphoSitePlus
K289 Ubiquitination PhosphoSitePlus
K319 Ubiquitination PhosphoSitePlus
Y321 Phosphorylation PhosphoSitePlus
Y325 Phosphorylation PhosphoSitePlus
K327 Ubiquitination PhosphoSitePlus
K341 Ubiquitination PhosphoSitePlus
T345 Phosphorylation PhosphoSitePlus
S347 Phosphorylation PhosphoSitePlus
S350 Phosphorylation PhosphoSitePlus
T353 Phosphorylation HPRD   phospho.ELM   PhosphoSitePlus 19651622, 18669648
T356 Phosphorylation P24941 (CDK2) , P11802 (CDK4) HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   UniProt 22094256, 10499802, 23186163, ...26265441, 18220336, 1756735, 24466208, 18669648, 23304241, 9139732, 9190208, 8626527
K359 Ubiquitination PhosphoSitePlus
S360 Phosphorylation PhosphoSitePlus
T373 Phosphorylation P06493 (CDK1) , P24941 (CDK2) , P11802 (CDK4) HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 22094256, 10499802, 23186163, ...15567975, 27419601, 8626527, 26159177, 1756735, 19690332, 19651622, 18220336, 9139732, 9190208, 12490153, 18768921
K420 Ubiquitination PhosphoSitePlus
K427 Acetylation PhosphoSitePlus
K427 Ubiquitination PhosphoSitePlus
K432 Ubiquitination PhosphoSitePlus
Y454 Phosphorylation PhosphoSitePlus
K537 Ubiquitination PhosphoSitePlus
K548 Acetylation PhosphoSitePlus
S567 Phosphorylation Q16539 (MAPK14) , P24941 (CDK2) HPRD   neXtProt   PhosphoSitePlus   RLIMS-P   Signor   UniProt 10499802, 9190208, 10207050, ...1756735, 8626527, 22279936, 27458253, 20871633
K574 Ubiquitination PhosphoSitePlus
T583 Phosphorylation PhosphoSitePlus
S588 Phosphorylation PhosphoSitePlus
T601 Phosphorylation HPRD   PhosphoSitePlus 20068231
Y606 Phosphorylation HPRD   phospho.ELM   PhosphoSitePlus 18669648
S608 Phosphorylation P24941 (CDK2) , P11802 (CDK4) HPRD   neXtProt   PhosphoSitePlus   RLIMS-P 10499802, 27419601, 27789274, ...10207050, 9010227, 1756735, 8626527, 12895470, 22322860, 23304241, 9190208, 15081408, 20068231
S612 Phosphorylation O96017 (CHEK2) , O14757 (CHEK1) , Q00534 (CDK6) , P11802 (CDK4) , P24941 (CDK2) HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 10499802, 10207050, 18784806, ...16107892, 11416152, 18669648, 29468543, 24466208, 16936279, 17380128, 22322860, 20068231, 19638577, 22682621, 9190208, 8626527, 1756735, 16360038
S618 Phosphorylation PhosphoSitePlus
S624 Phosphorylation PhosphoSitePlus   UniProt 23186163
T625 Phosphorylation PhosphoSitePlus
K640 Ubiquitination PhosphoSitePlus
K720 Sumoylation PhosphoSitePlus
T766 Phosphorylation PhosphoSitePlus
Y771 Phosphorylation PhosphoSitePlus
S773 Phosphorylation PhosphoSitePlus
T774 Phosphorylation PhosphoSitePlus
R775 Methylation PhosphoSitePlus
T778 Phosphorylation PhosphoSitePlus
S780 Phosphorylation P11802 (CDK4) , P30279 (CCND2) , P17612 (PRKACA) , Q00534 (CDK6) , Q96GD4 (AURKB) , P05771 (PRKCB) HPRD   neXtProt   PhosphoSitePlus   RLIMS-P   Signor   UniProt 15489884, 21270107, 27862183, ...10022898, 16936279, 11416152, 24169358, 11811957, 19551155, 24463355, 23505430, 16360038, 25737734, 23186163, 15809340, 18383818, 11306463, 24454925, 19638577, 15817163, 17621266, 27849562, 15547725, 30519351, 27419601, 26159177, 14676836, 20080185, 14732295, 19651622, 10086343, 22682621, 15548374, 19225156, 26203195, 11254678, 22493456, 23336272, 16236452, 23622063, 24466208, 9003781, 11805327, 17478427, 15941859, 29541385, 16326706, 28105458
R787 Methylation PhosphoSitePlus
S788 Phosphorylation Q00534 (CDK6) , P11802 (CDK4) HPRD   neXtProt   PhosphoSitePlus   UniProt 22094256, 23186163, 19664994, ...11416152, 19651622, 16936279, 22682621, 9139732, 9190208, 16360038
Y790 Phosphorylation PhosphoSitePlus
K791 Ubiquitination PhosphoSitePlus
S794 Phosphorylation PhosphoSitePlus
S795 Phosphorylation P05771 (PRKCB) , P11802 (CDK4) , P50750 (CDK9) , Q00534 (CDK6) , Q00535 (CDK5) HPRD   neXtProt   PRO   PhosphoSitePlus   RLIMS-P   Signor   UniProt 16718685, 27862183, 26250117, ...16936279, 15741232, 17896003, 9139732, 24463355, 16360038, 23186163, 15809340, 18383818, 21478909, 15138593, 11306463, 10069453, 15817163, 12490153, 15172998, 26106036, 15547725, 27419601, 26159177, 14676836, 12037672, 11416152, 14732295, 10210318, 22682621, 22094256, 15567975, 23336272, 12754735, 19664994, 18784806, 11805327, 15941859, 9190208, 22450712
R798 Methylation PhosphoSitePlus
Y805 Phosphorylation P00519 (ABL1) PhosphoSitePlus   Signor 16158058
S807 Phosphorylation P24941 (CDK2) , P50750 (CDK9) , Q00534 (CDK6) , P11802 (CDK4) , Q00526 (CDK3) , Q00535 (CDK5) , P06493 (CDK1) HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 24274149, 18938028, 28734226, ...16936279, 10499802, 16360038, 15741232, 31042625, 21115810, 24463355, 20703084, 25522274, 23186163, 15809340, 18383818, 1756735, 11306463, 25695870, 17568194, 19690332, 15084261, 12037672, 28169375, 20058876, 11416152, 15548374, 15194426, 22682621, 19651622, 22094256, 16236452, 9139732, 23622063, 18784806, 18669648, 22787429, 15941859, 9190208, 8626527, 20068231
K810 Methylation PhosphoSitePlus   UniProt 22787429
K810 Ubiquitination PhosphoSitePlus
S811 Phosphorylation Q00534 (CDK6) , P06493 (CDK1) , P24941 (CDK2) , P11802 (CDK4) , Q00526 (CDK3) , P50750 (CDK9) , Q00535 (CDK5) , Q13131 (PRKAA1) HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 18938028, 28734226, 16936279, ...10499802, 16360038, 15741232, 31042625, 9139732, 24463355, 20703084, 25522274, 23186163, 15809340, 18383818, 1756735, 11306463, 25695870, 17568194, 12037672, 15084261, 26159177, 28169375, 11416152, 15548374, 15194426, 22682621, 19217427, 19651622, 22094256, 16236452, 23622063, 21115810, 18784806, 18669648, 22787429, 15941859, 9190208, 8626527, 20068231
Y813 Phosphorylation HPRD   phospho.ELM   PhosphoSitePlus 18669648
K814 Ubiquitination PhosphoSitePlus
S816 Phosphorylation PhosphoSitePlus
T821 Phosphorylation P24941 (CDK2) , Q00534 (CDK6) , P05771 (PRKCB) HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 19228744, 16107892, 16936279, ...28105458, 9139732, 16360038, 23186163, 15809340, 18784806, 1756735, 29468543, 23315497, 19413330, 10499802, 11416152, 19651622, 20010939, 9315635, 22682621, 19664994, 18669648, 11805327, 9190208, 8626527, 20068231
T823 Phosphorylation HPRD   phospho.ELM   PhosphoSitePlus   UniProt 19413330, 23186163, 19664994, ...19651622, 18669648, 20068231
K824 Ubiquitination PhosphoSitePlus
T826 Phosphorylation Q00534 (CDK6) , P11802 (CDK4) , P24941 (CDK2) HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 1756735, 22094256, 10499802, ...23186163, 15809340, 9315635, 19664994, 25695870, 11416152, 19651622, 16936279, 18669648, 28105458, 22682621, 9139732, 9190208, 23737759, 8626527, 20068231, 16360038
S829 Phosphorylation PhosphoSitePlus
S834 Phosphorylation PhosphoSitePlus
S838 Phosphorylation PhosphoSitePlus
T841 Phosphorylation PhosphoSitePlus   UniProt 19690332
S842 Phosphorylation PhosphoSitePlus
K844 Ubiquitination PhosphoSitePlus
K847 Ubiquitination PhosphoSitePlus
S855 Phosphorylation HPRD   phospho.ELM   PhosphoSitePlus   UniProt 18669648, 23186163
R857 Methylation PhosphoSitePlus
K860 Methylation UniProt 20870719
K860 Ubiquitination PhosphoSitePlus
S862 Phosphorylation PhosphoSitePlus
S866 Phosphorylation PhosphoSitePlus
K870 Ubiquitination PhosphoSitePlus
K873 Acetylation PhosphoSitePlus   UniProt 20940255
K874 Acetylation PhosphoSitePlus   UniProt 20940255
S882 Phosphorylation PhosphoSitePlus
S895 Phosphorylation PhosphoSitePlus
K896 Acetylation PhosphoSitePlus
K896 Ubiquitination PhosphoSitePlus
K900 Ubiquitination PhosphoSitePlus
T905 Phosphorylation PhosphoSitePlus
S919 Phosphorylation PhosphoSitePlus
K925 Acetylation PhosphoSitePlus
Site PTM Type PTM Enzyme Score Source PMID
K873 Acetylation PR:000007102 (EP300) PRO 16374512
K874 Acetylation PR:000007102 (EP300) PRO 16374512


Proteoforms

PRO ID (Short Label) Sites PTM Enzyme Source PMID
PR:000049016 (hRB1/InitMet-/Phos:2) pS795 PRO
PR:000049017 (hRB1/InitMet-/PhosRes+) p PRO
PR:000028788 (hRB1/iso:1/Ac:1) acK873,acK874 PR:000007102 (EP300) PRO 16374512


PTM-dependent PPI

PTM type Substrate Site Interactant Association type Source PMID
Phosphorylation P06400 (RB1) S811 P00519 (ABL1) unknown eFIP 21445688
Phosphorylation P06400 (RB1) S608 Q13526 (PIN1) unknown eFIP 22322860
Phosphorylation Q01094 (E2F1) S337 P06400 (RB1) increased_association eFIP 20616879
Phosphorylation Q01094 (E2F1) S332 P06400 (RB1) inhibited_association eFIP 25101673
Phosphorylation Q01094 (E2F1) S375 P06400 (RB1) increased_association eFIP 7824278


Proteoform PPIs

Protein 1 Relation Protein 2 Source PMID
PR:P29590-3 (hPML/iso:3) Interaction PR:P06400 (hRB1) PRO 9448006
PR:P29590-5 (hPML/iso:5) Interaction PR:P06400 (hRB1) PRO 9448006


PTM sites affected in variants

Site Variant Source PMID Disease [Sample source]
T5 S5 Biomuta DOID:1909 / melanoma [ cosmic ]
K63 T63 Biomuta DOID:363 / uterine cancer [ tcga ]
K63 I63 Biomuta DOID:1909 / melanoma [ cosmic ]
K63 * Biomuta DOID:1612 / breast cancer [ cosmic ]
K65 N65 Biomuta DOID:9256 / colorectal cancer [ tcga ] DOID:1909 / melanoma [ cosmic ]
K94 N94 Biomuta DOID:363 / uterine cancer [ cosmic, icgc, tcga ]
S149 * Biomuta DOID:11054 / urinary bladder cancer [ tcga ]
S163 N163 Biomuta DOID:1115 / sarcoma [ tcga ]
S230 * Biomuta DOID:1612 / breast cancer [ cosmic, icgc ]
S249 * Biomuta DOID:1909 / melanoma [ icgc, tcga ]
K289 * Biomuta DOID:1909 / melanoma [ tcga ] DOID:1324 / lung cancer [ cosmic ]
Y321 * Biomuta DOID:1612 / breast cancer [ cosmic ]
K341 * Biomuta DOID:1793 / pancreatic cancer [ icgc ]
S350 I350 Biomuta DOID:1324 / lung cancer [ tcga ]
K427 * Biomuta DOID:1324 / lung cancer [ tcga ]
K432 * Biomuta DOID:3571 / liver cancer [ icgc ] DOID:0070003 / blastoma [ cosmic ]
Y454 H454 Biomuta DOID:363 / uterine cancer [ tcga ]
Y454 * Biomuta DOID:1324 / lung cancer [ cosmic ]
K548 T548 Biomuta DOID:10283 / prostate cancer [ cosmic, icgc ]
K548 N548 Biomuta DOID:1909 / melanoma [ cosmic ]
S567 L567 Biomuta DOID:1324 / lung cancer [ cosmic ] DOID:11054 / urinary bladder cancer [ tcga ]
S567 * Biomuta DOID:1324 / lung cancer [ cosmic ]
S588 F588 Biomuta DOID:1793 / pancreatic cancer [ icgc ] DOID:1324 / lung cancer [ cosmic ]
Y606 * Biomuta DOID:1612 / breast cancer [ cosmic, icgc, tcga ]
S612 P612 Biomuta DOID:10283 / prostate cancer [ cosmic ]
S618 * Biomuta DOID:1324 / lung cancer [ cosmic ]
S624 Y624 Biomuta DOID:363 / uterine cancer [ tcga ]
K720 * Biomuta DOID:1324 / lung cancer [ cosmic ]
T778 A778 Biomuta DOID:3070 / malignant glioma [ clinvar ]
S780 * Biomuta DOID:1324 / lung cancer [ cosmic, tcga ] DOID:11054 / urinary bladder cancer [ tcga ]
R787 Q787 Biomuta DOID:363 / uterine cancer [ cosmic, icgc, tcga ]
R787 * Biomuta DOID:1612 / breast cancer [ tcga ] DOID:1324 / lung cancer [ tcga ] DOID:3070 / malignant glioma [ clinvar ] DOID:4362 / cervical cancer [ tcga ] DOID:2394 / ovarian cancer [ icgc, tcga ] DOID:1115 / sarcoma [ tcga ]
S788 N788 Biomuta DOID:10534 / stomach cancer [ cosmic, icgc, tcga ]
Y790 * Biomuta DOID:5041 / esophageal cancer [ icgc ] DOID:1324 / lung cancer [ cosmic ]
K791 * Biomuta DOID:1324 / lung cancer [ cosmic ]
S794 I794 Biomuta DOID:11054 / urinary bladder cancer [ cosmic ]
S795 * Biomuta DOID:3070 / malignant glioma [ cosmic, icgc, tcga ]
R798 W798 Biomuta DOID:3963 / thyroid carcinoma [ cosmic, icgc, tcga ] DOID:2994 / germ cell cancer [ cosmic ]
R798 Q798 Biomuta DOID:1909 / melanoma [ cosmic ]
S816 * Biomuta DOID:11054 / urinary bladder cancer [ tcga ]
S829 * Biomuta DOID:1612 / breast cancer [ icgc, tcga ] DOID:1324 / lung cancer [ cosmic ] DOID:11054 / urinary bladder cancer [ tcga ]
S834 * Biomuta DOID:4362 / cervical cancer [ tcga ] DOID:3571 / liver cancer [ icgc ] DOID:1324 / lung cancer [ cosmic ] DOID:11054 / urinary bladder cancer [ tcga ]
S838 L838 Biomuta DOID:1612 / breast cancer [ cosmic, icgc ]
K844 * Biomuta DOID:11054 / urinary bladder cancer [ cosmic, tcga ]
R857 H857 Biomuta DOID:363 / uterine cancer [ cosmic, icgc, tcga ]
R857 C857 Biomuta DOID:363 / uterine cancer [ tcga ]
K860 R860 Biomuta DOID:9256 / colorectal cancer [ tcga ]
K873 T873 Biomuta DOID:1909 / melanoma [ cosmic ]
K873 E873 Biomuta DOID:1612 / breast cancer [ cosmic, icgc, tcga ]