Protein Information

Cytoscape View


UniProt AC / UniProt ID O43683 / BUB1_HUMAN
Protein Name Mitotic checkpoint serine/threonine-protein kinase BUB1
Gene Name Name: BUB1
Synonyms:BUB1L;
Organism Homo sapiens (Human)

PRO ID PR:O43683
DAG View
PRO Name mitotic checkpoint serine/threonine-protein kinase BUB1 (human)
Definition A mitotic checkpoint serine/threonine-protein kinase BUB1 that is encoded in the genome of human.
Short Label hBUB1
Category organism-gene

Interactive Sequence View

Select/align proteoforms across species


O43683 (BUB1) as Substrate



Site PTM Type PTM Enzyme Score Source PMID
S17 Phosphorylation PhosphoSitePlus
T47 Phosphorylation PhosphoSitePlus
K60 Acetylation PhosphoSitePlus
T148 Phosphorylation O43683 (BUB1) PhosphoSitePlus 26399325
T173 Phosphorylation O43683 (BUB1) PhosphoSitePlus 26399325
K175 Ubiquitination PhosphoSitePlus
S176 Phosphorylation O43683 (BUB1) PhosphoSitePlus 26399325
K187 Ubiquitination PhosphoSitePlus
S194 Phosphorylation PhosphoSitePlus
S198 Phosphorylation O43683 (BUB1) PhosphoSitePlus 26399325
K203 Ubiquitination PhosphoSitePlus
T213 Phosphorylation O43683 (BUB1) PhosphoSitePlus 26399325
K216 Ubiquitination PhosphoSitePlus
Y219 Phosphorylation PhosphoSitePlus
S220 Phosphorylation PhosphoSitePlus
K241 Ubiquitination PhosphoSitePlus
S247 Phosphorylation PhosphoSitePlus   RLIMS-P 26399325
S250 Phosphorylation PhosphoSitePlus   RLIMS-P 26399325
K264 Ubiquitination PhosphoSitePlus
K277 Ubiquitination PhosphoSitePlus
K279 Ubiquitination PhosphoSitePlus
K290 Ubiquitination PhosphoSitePlus
K292 Ubiquitination PhosphoSitePlus
K299 Ubiquitination PhosphoSitePlus
T306 Phosphorylation PhosphoSitePlus
S307 Phosphorylation phospho.ELM   PhosphoSitePlus   UniProt 18669648, 23186163
S314 Phosphorylation Q13315 (ATM) phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 19369195, 17525332, 22071147, ...22099307
S331 Phosphorylation PhosphoSitePlus   UniProt 23186163
K353 Ubiquitination PhosphoSitePlus
S375 Phosphorylation PhosphoSitePlus   UniProt 19369195
T378 Phosphorylation PhosphoSitePlus
S402 Phosphorylation PhosphoSitePlus
K403 Ubiquitination PhosphoSitePlus
K410 Ubiquitination PhosphoSitePlus
S411 Phosphorylation PhosphoSitePlus
K416 Ubiquitination PhosphoSitePlus
S419 Phosphorylation O43683 (BUB1) PhosphoSitePlus 26399325
T435 Phosphorylation PhosphoSitePlus
S436 Phosphorylation phospho.ELM   PhosphoSitePlus 18669648
S437 Phosphorylation PhosphoSitePlus
T440 Phosphorylation PhosphoSitePlus
T441 Phosphorylation PhosphoSitePlus
T444 Phosphorylation PhosphoSitePlus
S445 Phosphorylation phospho.ELM   PhosphoSitePlus 18669648
T452 Phosphorylation phospho.ELM   PhosphoSitePlus 22067460
S454 Phosphorylation O43683 (BUB1) PhosphoSitePlus 26399325
K455 Ubiquitination PhosphoSitePlus
S459 Phosphorylation PhosphoSitePlus   RLIMS-P 28072388, 28604727
T461 Phosphorylation PhosphoSitePlus   RLIMS-P 28072388, 28604727
T479 Phosphorylation PhosphoSitePlus
K506 Ubiquitination PhosphoSitePlus
S510 Phosphorylation O43683 (BUB1) PhosphoSitePlus 26399325
S511 Phosphorylation O43683 (BUB1) PhosphoSitePlus 26399325
S525 Phosphorylation O43683 (BUB1) PhosphoSitePlus   UniProt 26399325, 23186163
K535 Ubiquitination PhosphoSitePlus
Y538 Phosphorylation PhosphoSitePlus
T552 Phosphorylation O43683 (BUB1) PhosphoSitePlus 26399325
S557 Phosphorylation PhosphoSitePlus
S563 Phosphorylation O43683 (BUB1) PhosphoSitePlus   UniProt 19369195, 26399325, 19690332
K588 Ubiquitination PhosphoSitePlus
T589 Phosphorylation O43683 (BUB1) phospho.ELM   PhosphoSitePlus   RLIMS-P 18669648, 26399325
S593 Phosphorylation P06493 (CDK1) neXtProt   phospho.ELM   PhosphoSitePlus   UniProt 23186163, 22067460, 18669648, ...16760428, 19690332, 20068231
K595 Ubiquitination PhosphoSitePlus
S596 Phosphorylation phospho.ELM   PhosphoSitePlus   UniProt 18691976, 21406692, 23186163, ...19369195, 22067460, 18669648, 19690332, 20068231
T601 Phosphorylation PhosphoSitePlus
S602 Phosphorylation phospho.ELM   PhosphoSitePlus 18669648
S608 Phosphorylation phospho.ELM   PhosphoSitePlus 18669648
T609 Phosphorylation P06493 (CDK1) neXtProt   PhosphoSitePlus   RLIMS-P   Signor   UniProt 26912231, 16760428
K613 Ubiquitination PhosphoSitePlus
S618 Phosphorylation O43683 (BUB1) PhosphoSitePlus 26399325
K625 Ubiquitination PhosphoSitePlus
S640 Phosphorylation PhosphoSitePlus
K653 Ubiquitination PhosphoSitePlus
S655 Phosphorylation phospho.ELM   PhosphoSitePlus   UniProt 18691976, 18669648, 23186163, ...19369195
K660 Ubiquitination PhosphoSitePlus
S661 Phosphorylation phospho.ELM   PhosphoSitePlus   UniProt 18669648, 23186163
K663 Ubiquitination PhosphoSitePlus
S667 Phosphorylation O43683 (BUB1) PhosphoSitePlus 26399325
S668 Phosphorylation PhosphoSitePlus   UniProt 23186163
Y671 Phosphorylation PhosphoSitePlus
S672 Phosphorylation PhosphoSitePlus   UniProt 23186163
S679 Phosphorylation O43683 (BUB1) PhosphoSitePlus   RLIMS-P 26399325
S752 Phosphorylation O43683 (BUB1) PhosphoSitePlus 26399325
S755 Phosphorylation PhosphoSitePlus
K756 Ubiquitination PhosphoSitePlus
K770 Ubiquitination PhosphoSitePlus
K777 Ubiquitination PhosphoSitePlus
T778 Phosphorylation PhosphoSitePlus
K785 Ubiquitination PhosphoSitePlus
K813 Ubiquitination PhosphoSitePlus
K821 Ubiquitination PhosphoSitePlus
Y869 Phosphorylation PhosphoSitePlus
S870 Phosphorylation PhosphoSitePlus
K958 Ubiquitination PhosphoSitePlus
T960 Phosphorylation O43683 (BUB1) PhosphoSitePlus 26399325
S969 Phosphorylation O43683 (BUB1) phospho.ELM   PhosphoSitePlus   RLIMS-P 22067460, 26399325, 26658523
K1007 Ubiquitination PhosphoSitePlus
K1014 Ubiquitination PhosphoSitePlus
K1056 Ubiquitination PhosphoSitePlus
K1065 Ubiquitination PhosphoSitePlus
K1081 Ubiquitination PhosphoSitePlus
Site PTM Type PTM Enzyme Score Source PMID
Phosphorylation PR:P53350 (hPLK1) , PR:000003271 (CDK1) PRO 16760428
S159 Phosphorylation HPRD 19007248
T306 Phosphorylation HPRD 18691976
S307 Phosphorylation HPRD 18669648
S314 Phosphorylation PR:000035423 (hATM/Phos:2) HPRD   PRO 17525332, 22071147, 22099307
S375 Phosphorylation HPRD 18691976, 20068231
S436 Phosphorylation HPRD 18669648
T440 Phosphorylation HPRD 20068231
S445 Phosphorylation HPRD 18669648, 20068231
T452 Phosphorylation HPRD 19007248
T461 Phosphorylation HPRD 18691976
S511 Phosphorylation HPRD 19691289
S563 Phosphorylation HPRD 20068231
T589 Phosphorylation HPRD 18669648, 20068231
S593 Phosphorylation PR:000003271 (CDK1) HPRD   PRO 18691976, 18669648, 16760428, ...20068231
S596 Phosphorylation HPRD 19651622, 18691976, 18669648, ...20068231
S602 Phosphorylation HPRD 18669648
S608 Phosphorylation HPRD 18669648
T609 Phosphorylation PR:000003271 (CDK1) , P06493 (CDK1) , PR:P53350 (hPLK1) HPRD   PRO 16760428
S655 Phosphorylation HPRD 18691976, 19691289, 19651622, ...17322306, 18669648, 20068231
S661 Phosphorylation HPRD 18669648, 20068231
S755 Phosphorylation HPRD 20068231
S969 Phosphorylation HPRD 19413330


O43683 (BUB1) as PTM Enzyme



Substrate Site Score Source PMID
O43683 (BUB1) T148 PhosphoSitePlus 26399325
O43683 (BUB1) T173 PhosphoSitePlus 26399325
O43683 (BUB1) S176 PhosphoSitePlus 26399325
O43683 (BUB1) S198 PhosphoSitePlus 26399325
O43683 (BUB1) T213 PhosphoSitePlus 26399325
O43683 (BUB1) S419 PhosphoSitePlus 26399325
O43683 (BUB1) S454 PhosphoSitePlus 26399325
O43683 (BUB1) S510 PhosphoSitePlus 26399325
O43683 (BUB1) S511 PhosphoSitePlus 26399325
O43683 (BUB1) S525 PhosphoSitePlus 26399325
O43683 (BUB1) T552 PhosphoSitePlus 26399325
O43683 (BUB1) S563 PhosphoSitePlus 26399325
O43683 (BUB1) T589 PhosphoSitePlus 26399325
O43683 (BUB1) S618 PhosphoSitePlus 26399325
O43683 (BUB1) S667 PhosphoSitePlus 26399325
O43683 (BUB1) S679 PhosphoSitePlus 26399325
O43683 (BUB1) S752 PhosphoSitePlus 26399325
O43683 (BUB1) T960 PhosphoSitePlus 26399325
O43683 (BUB1) S969 PhosphoSitePlus 26658523
O43684 (BUB3) S19 PhosphoSitePlus 20057499
Q12834 (CDC20) S41 HPRD   PhosphoSitePlus   Signor 15525512, 19413330, 18669648
Q12834 (CDC20) S72 HPRD   PhosphoSitePlus   Signor 15525512, 18767875
Q12834 (CDC20) S92 HPRD   PhosphoSitePlus   Signor 15525512
Q12834 (CDC20) S153 HPRD   PhosphoSitePlus   Signor 15525512, 18995837
Q12834 (CDC20) T157 HPRD   PhosphoSitePlus   Signor 15525512
Q12834 (CDC20) S161 HPRD   PhosphoSitePlus   Signor 15525512


Proteoforms

PRO ID (Short Label) Sites PTM Enzyme Source PMID
PR:000035412 (hBUB1/Phos:7) pS593,pT609 PR:000003271 (CDK1) PRO 16760428
PR:000035418 (hBUB1/Phos:8) pT609,p PR:P53350 (hPLK1) PRO 16760428
PR:000035418 (hBUB1/Phos:8) pT609,p PR:000003271 (CDK1) PRO 16760428
PR:000035421 (hBUB1/Phos:10) pS314 PR:000035423 (hATM/Phos:2) PRO 22071147, 22099307


PTM-dependent PPI

PTM type Substrate Site Interactant Association type Source PMID
Phosphorylation O43683 (BUB1) T609 P53350 (PLK1) unknown eFIP 26912231


Proteoform PPIs

Protein 1 Relation Protein 2 Source PMID
PR:000035405 (hBUB1/PhosRes-) NOT Interaction PR:P53350 (hPLK1) PRO 16760428
PR:000035412 (hBUB1/Phos:7) increased Interaction PR:Q9NQS7 (hINCENP) PRO 16760428
PR:000035428 (hBUB1B/Phos:2) increased Interaction PR:O43683 (hBUB1) PRO 11792804
PR:O43683 (hBUB1) Interaction PR:P25054 (hAPC) PRO
PR:O43683 (hBUB1) Interaction PR:P04637-1 (hTP53/iso:1) PRO 19252416
PR:O43683 (hBUB1) Interaction PR:O60566 (hBUB1B) PRO 11792804
PR:O43683 (hBUB1) Interaction PR:Q8NG31 (hKNL1) PRO
PR:O43683 (hBUB1) Interaction PR:P53350 (hPLK1) PRO
PR:O43683 (hBUB1) Interaction PR:O43684 (hBUB3) PRO 10198256, 15525512
PR:O43683 (hBUB1) Interaction PR:Q9Y6D9 (hMAD1L1) PRO 10198256
PR:O43684 (hBUB3) Interaction PR:O43683 (hBUB1) PRO 10198256
PR:O60566 (hBUB1B) Interaction PR:O43683 (hBUB1) PRO 11792804
PR:P04637-1 (hTP53/iso:1) Interaction PR:O43683 (hBUB1) PRO 19252416
PR:P25054 (hAPC) Interaction PR:O43683 (hBUB1) PRO 11283619
PR:P53350 (hPLK1) Interaction PR:O43683 (hBUB1) PRO
PR:Q8NG31 (hKNL1) Interaction PR:O43683 (hBUB1) PRO
PR:Q9Y6D9 (hMAD1L1) Interaction PR:O43683 (hBUB1) PRO 10198256


PTM sites affected in variants

Site Variant Source PMID Disease [Sample source]
S159 * Biomuta DOID:5041 / esophageal cancer [ icgc ]
S176 L176 Biomuta DOID:1909 / melanoma [ tcga ]
S220 F220 Biomuta DOID:3070 / malignant glioma [ tcga ]
S250 P250 Biomuta DOID:1909 / melanoma [ tcga ]
K299 T299 Biomuta DOID:10534 / stomach cancer [ cosmic, icgc, tcga ]
K299 E299 Biomuta DOID:3070 / malignant glioma [ cosmic, icgc, tcga ]
T378 A378 Biomuta DOID:9256 / colorectal cancer [ tcga ] DOID:1909 / melanoma [ cosmic ]
S437 Y437 Biomuta DOID:363 / uterine cancer [ icgc, tcga ] DOID:9256 / colorectal cancer [ tcga ]
T452 M452 Biomuta DOID:3070 / malignant glioma [ tcga ]
S459 P459 Biomuta DOID:263 / kidney cancer [ tcga ]
T461 A461 Biomuta DOID:1909 / melanoma [ cosmic ]
K506 N506 Biomuta DOID:363 / uterine cancer [ tcga ]
S511 T511 Biomuta DOID:3070 / malignant glioma [ tcga ]
S525 F525 Biomuta DOID:9256 / colorectal cancer [ tcga ]
T552 I552 Biomuta DOID:363 / uterine cancer [ cosmic, icgc, tcga ]
T589 N589 Biomuta DOID:3571 / liver cancer [ icgc ]
K595 R595 Biomuta DOID:3571 / liver cancer [ icgc ] DOID:0070003 / blastoma [ cosmic ]
S602 C602 Biomuta DOID:11054 / urinary bladder cancer [ tcga ]
S602 Y602 Biomuta DOID:363 / uterine cancer [ tcga ]
S640 Y640 Biomuta DOID:363 / uterine cancer [ tcga ]
S667 L667 Biomuta DOID:9256 / colorectal cancer [ icgc, tcga ] DOID:10534 / stomach cancer [ tcga ] DOID:1909 / melanoma [ cosmic, tcga ]
S668 F668 Biomuta DOID:1612 / breast cancer [ tcga ]
Y671 H671 Biomuta DOID:5041 / esophageal cancer [ icgc ]
S752 F752 Biomuta DOID:1909 / melanoma [ icgc, tcga ]