iPTMnet Report for O15530 (PDPK1)


Protein Information

Cytoscape View


UniProt AC / UniProt ID O15530 / PDPK1_HUMAN
Protein Name 3-phosphoinositide-dependent protein kinase 1
Gene Name Name: PDPK1
Synonyms:PDK1;
Organism Homo sapiens (Human)

PRO ID PR:O15530
DAG View
PRO Name 3-phosphoinositide-dependent protein kinase 1 (human)
Definition A 3-phosphoinositide-dependent protein kinase 1 that is encoded in the genome of human.
Short Label hPDPK1
Category organism-gene

Interactive Sequence View

Select/align proteoforms across species


O15530 (PDPK1) as Substrate



Site PTM Type PTM Enzyme Score Source PMID
T5 O-Glycosylation GlyGen 36580660
S6 O-Glycosylation GlyGen 36580660
Y9 Phosphorylation neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 11481331, 16314505, 15302935, ...14585963, 12738763, 18024423, 21537480
Y9 Phosphorylation P07949 (RET) neXtProt   phospho.ELM   PhosphoSitePlus 12738763
Y9 Phosphorylation P12931 (SRC) neXtProt   phospho.ELM   Signor 11481331, 14585963
S22 O-Glycosylation GlyGen 36580660
S22 Phosphorylation PhosphoSitePlus
S25 Phosphorylation neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 10455013
S25 Phosphorylation O15530 (PDPK1) PhosphoSitePlus   Signor 10455013
T33 Phosphorylation neXtProt   PhosphoSitePlus 11481331
T33 Phosphorylation P12931 (SRC) neXtProt 11481331
S36 Phosphorylation PhosphoSitePlus
T37 Phosphorylation phospho.ELM   PhosphoSitePlus 18669648
S45 O-Glycosylation GlyGen 36580660
S45 Phosphorylation PhosphoSitePlus
S64 Phosphorylation PhosphoSitePlus
K83 Ubiquitination PhosphoSitePlus
S92 O-Glycosylation GlyGen 36580660
S92 Phosphorylation PhosphoSitePlus
S105 O-Glycosylation GlyGen 36580660
K111 Ubiquitination PhosphoSitePlus
K123 Ubiquitination PhosphoSitePlus
T148 Phosphorylation PhosphoSitePlus
Y156 Phosphorylation PhosphoSitePlus
S160 Phosphorylation PhosphoSitePlus
Y161 Phosphorylation PhosphoSitePlus
S176 Phosphorylation PhosphoSitePlus
T180 Phosphorylation PhosphoSitePlus
C181 Methylation IEDB 31844290
T222 O-Glycosylation GlyGen 36580660
S231 Phosphorylation PhosphoSitePlus
S234 Phosphorylation PhosphoSitePlus
K235 Ubiquitination PhosphoSitePlus
S241 Phosphorylation neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 21565140, 12177059, 10455013, ...11481331, 22039492, 15302935, 18669648, 16780920, 15772071
S241 Phosphorylation O15530 (PDPK1) neXtProt   PhosphoSitePlus   Signor   UniProt 12177059, 16780920, 10455013, ...11481331
T245 Phosphorylation phospho.ELM   PhosphoSitePlus 17081983
Y248 Phosphorylation PhosphoSitePlus
S250 Phosphorylation PhosphoSitePlus
T255 Phosphorylation PhosphoSitePlus
K257 Ubiquitination PhosphoSitePlus
Y288 Phosphorylation PhosphoSitePlus
K304 Acetylation PhosphoSitePlus
K304 Ubiquitination PhosphoSitePlus
K315 Ubiquitination PhosphoSitePlus
K323 Ubiquitination PhosphoSitePlus
K337 Ubiquitination PhosphoSitePlus
T354 O-Glycosylation GlyGen 36580660
T354 Phosphorylation neXtProt   PhosphoSitePlus   UniProt 22544756
T354 Phosphorylation Q14680 (MELK) neXtProt 22544756
S366 O-Glycosylation GlyGen 36580660
S366 Phosphorylation PhosphoSitePlus
Y373 Phosphorylation phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 11481331, 16314505, 15302935, ...14585963, 20044479, 18024423, 11054117, 20643654, 21537480
Y373 Phosphorylation P08069 (IGF1R) PhosphoSitePlus   Signor 20044479, 20643654
Y373 Phosphorylation P12931 (SRC) phospho.ELM   PhosphoSitePlus   Signor 18024423, 11054117, 20643654, ...11481331
Y376 Phosphorylation neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 11481331, 16314505, 15302935, ...14585963, 20044479, 18024423, 20643654, 21537480
Y376 Phosphorylation P08069 (IGF1R) PhosphoSitePlus   Signor 20044479, 20643654
Y376 Phosphorylation P12931 (SRC) neXtProt   phospho.ELM   PhosphoSitePlus   Signor 18024423, 20643654, 11481331, ...14585963
S381 O-Glycosylation GlyGen 36580660
S390 O-Glycosylation GlyGen 36580660
S392 O-Glycosylation GlyGen 36580660
S393 Phosphorylation phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 10455013
S393 Phosphorylation O15530 (PDPK1) PhosphoSitePlus   Signor 10455013
S394 O-Glycosylation GlyGen 36580660
S394 Phosphorylation neXtProt   PhosphoSitePlus   RLIMS-P   UniProt 19920149, 22544756
S394 Phosphorylation Q99683 (MAP3K5) neXtProt   PhosphoSitePlus 19920149, 22544756
S396 O-Glycosylation GlyGen 36580660
S396 Phosphorylation neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 15743829, 10455013
S396 Phosphorylation O15530 (PDPK1) PhosphoSitePlus   Signor 15743829, 10455013
S398 Phosphorylation neXtProt   PhosphoSitePlus   RLIMS-P   UniProt 19920149, 22544756
S398 Phosphorylation Q99683 (MAP3K5) neXtProt   PhosphoSitePlus 19920149, 22544756
S400 O-Glycosylation GlyGen 36580660
T402 O-Glycosylation GlyGen 36580660
S408 O-Glycosylation GlyGen 36580660
S410 Phosphorylation neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 10455013, 11481331
S410 Phosphorylation O15530 (PDPK1) PhosphoSitePlus   Signor 10455013
S423 O-Glycosylation GlyGen 36580660
K441 Ubiquitination PhosphoSitePlus
Y485 Phosphorylation neXtProt   PhosphoSitePlus 11481331
Y485 Phosphorylation P12931 (SRC) neXtProt 11481331
Y486 Phosphorylation PhosphoSitePlus
K495 Ubiquitination PhosphoSitePlus
S501 Phosphorylation neXtProt   PhosphoSitePlus 19047061
S501 Phosphorylation Q04759 (PRKCQ) neXtProt 19047061
K509 Ubiquitination PhosphoSitePlus
T513 Phosphorylation neXtProt   phospho.ELM   PhosphoSitePlus   UniProt 16780920, 11481331
T513 Phosphorylation O15530 (PDPK1) PhosphoSitePlus   UniProt 16780920
T513 Phosphorylation P12931 (SRC) neXtProt 11481331
S529 Phosphorylation neXtProt 19047061
S529 Phosphorylation O15530 (PDPK1) neXtProt 19047061
S529 Phosphorylation Q04759 (PRKCQ) neXtProt 19047061
K534 Acetylation PhosphoSitePlus
S549 Phosphorylation PhosphoSitePlus
Site PTM Type PTM Enzyme Score Source PMID
Y9 Phosphorylation HPRD 11481331
Y9 Phosphorylation P12931 (SRC) HPRD 11481331
S25 Phosphorylation HPRD 10455013
T33 Phosphorylation HPRD 11481331
T33 Phosphorylation O15530 (PDPK1) HPRD 11481331
T37 Phosphorylation HPRD 19413330, 18691976, 18669648
S241 Phosphorylation HPRD 19413330, 18691976, 10455013, ...20230923, 20058876, 19664994, 19664995, 15144186, 18452278, 19651622, 18669648, 20166139, 19415658, 20068231
S241 Phosphorylation O15530 (PDPK1) HPRD 19413330, 18691976, 10455013, ...20230923, 20058876, 19664994, 19664995, 15144186, 18452278, 19651622, 18669648, 20166139, 19415658, 20068231
T245 Phosphorylation HPRD 17081983
Y373 Phosphorylation HPRD 11481331
Y373 Phosphorylation P12931 (SRC) HPRD 11481331
S393 Phosphorylation HPRD 10455013
S396 Phosphorylation HPRD 10455013
S410 Phosphorylation HPRD 10455013
Y485 Phosphorylation HPRD 11481331
Y485 Phosphorylation P12931 (SRC) HPRD 11481331
S501 Phosphorylation HPRD 19664994
T513 Phosphorylation HPRD 11481331
T513 Phosphorylation O15530 (PDPK1) HPRD 11481331
Site PTM Type PTM Enzyme Score Source PMID
Y9 Phosphorylation HPRD 11481331
Y9 Phosphorylation P12931 (SRC) HPRD 11481331
S25 Phosphorylation HPRD 10455013
T33 Phosphorylation HPRD 11481331
T33 Phosphorylation O15530 (PDPK1) HPRD 11481331
T37 Phosphorylation HPRD   PhosphoSitePlus 19413330, 18691976, 18669648
S114 Phosphorylation HPRD   PhosphoSitePlus 19413330, 10455013, 20230923, ...19664994, 20058876, 19664995, 15144186, 18452278, 19651622, 18691976, 18669648, 20166139, 19415658, 20068231
S114 Phosphorylation O15530 (PDPK1) HPRD 19413330, 18691976, 10455013, ...20230923, 20058876, 19664994, 19664995, 15144186, 18452278, 19651622, 18669648, 20166139, 19415658, 20068231
T118 Phosphorylation HPRD 17081983
Y246 Phosphorylation HPRD 11481331
Y246 Phosphorylation P12931 (SRC) HPRD 11481331
S266 Phosphorylation HPRD 10455013
S269 Phosphorylation HPRD 10455013
S283 Phosphorylation HPRD 10455013
K314 Ubiquitination PhosphoSitePlus
Y358 Phosphorylation HPRD 11481331
Y358 Phosphorylation P12931 (SRC) HPRD 11481331
S374 Phosphorylation HPRD 19664994
T386 Phosphorylation HPRD 11481331
T386 Phosphorylation O15530 (PDPK1) HPRD 11481331
K407 Acetylation PhosphoSitePlus


O15530 (PDPK1) as PTM Enzyme



Substrate Site Score Source PMID
O00141 (SGK1) T256 HPRD   neXtProt   PhosphoSitePlus   RLIMS-P+   Signor 10357815, 15209375, 25384981, ...10191262
O00141 (SGK1) S422 HPRD   neXtProt 10191262
O14920 (IKBKB) S181 neXtProt 16207722
O15530 (PDPK1) S25 PhosphoSitePlus   Signor 10455013
O15530-4 (PDPK1) T33 HPRD 11481331
O15530-4 (PDPK1) S114 HPRD 19413330, 18691976, 10455013, ...20230923, 20058876, 19664994, 19664995, 15144186, 18452278, 19651622, 18669648, 20166139, 19415658, 20068231
O15530 (PDPK1) S241 HPRD   neXtProt   PhosphoSitePlus   Signor   UniProt 19413330, 18691976, 12177059, ...10455013, 11481331, 20230923, 20058876, 19664994, 19664995, 15144186, 18452278, 19651622, 18669648, 16780920, 20166139, 19415658, 20068231
O15530-4 (PDPK1) T386 HPRD 11481331
O15530 (PDPK1) S393 PhosphoSitePlus   Signor 10455013
O15530 (PDPK1) S396 PhosphoSitePlus   Signor 15743829, 10455013
O15530 (PDPK1) S410 PhosphoSitePlus   Signor 10455013
O15530-1 (PDPK1) T513 HPRD   PhosphoSitePlus   UniProt 16780920, 11481331
O15530 (PDPK1) S529 neXtProt 19047061
P05106-1 (ITGB3) T779 HPRD   PhosphoSitePlus 18578522, 10896934
P05771 (PRKCB) T500 neXtProt   Signor 17115692, 9889098
P08559 (PDHA1) S232 PhosphoSitePlus 11485553
P08559 (PDHA1) S293 PhosphoSitePlus 11485553
P08559 (PDHA1) S300 PhosphoSitePlus 11485553
P17612 (PRKACA) T198 neXtProt 9707564
P23443-2 (RPS6KB1) T229 PhosphoSitePlus 19570988
P23443 (RPS6KB1) T252 HPRD   neXtProt   PhosphoSitePlus   Signor 9427642, 19864428, 12183455, ...9445476, 9271440, 10601311
P23443 (RPS6KB1) T412 HPRD   PhosphoSitePlus   Signor 9271440, 12183455, 9445476, ...10601311
P31749 (AKT1) T34 RLIMS-P+ 25912234
P31749 (AKT1) T308 HPRD   neXtProt   PhosphoSitePlus   RLIMS-P+   Signor 24352480, 10226025, 19602588, ...10698939, 12167717, 25912234, 11896604, 16306447, 20361045, 21798082, 26235620, 9445477, 9368760, 9094314, 15718470, 20643654, 12000750, 12808134
P31749-1 (AKT1) S473 HPRD   PhosphoSitePlus   Signor 10226025, 11896604
P31751 (AKT2) T309 HPRD   PhosphoSitePlus   RLIMS-P+   Signor 15191648, 12048203, 15743829, ...9512493, 9374542
P31751-1 (AKT2) S474 HPRD   Signor 15743829, 12048203, 9374542
P36507 (MAP2K2) S222 neXtProt 15175348
P36507 (MAP2K2) S226 neXtProt   PhosphoSitePlus   Signor 15175348
P51812 (RPS6KA3) S227 neXtProt   Signor 10480933, 19956600
P51812 (RPS6KA3) S386 neXtProt 10480933
Q02156 (PRKCE) T566 HPRD   neXtProt   PhosphoSitePlus   Signor 11964154, 10945988, 11062054, ...12640464
Q02750 (MAP2K1) S218 neXtProt 15175348
Q02750 (MAP2K1) S222 neXtProt   PhosphoSitePlus   Signor 15175348
Q05513-2 (PRKCZ) T227 HPRD 10357815, 11222751, 14500673, ...9748166, 18691976
Q05513 (PRKCZ) T410 HPRD   neXtProt   PhosphoSitePlus   Signor 11141077, 11222751, 14500673, ...9748166, 18691976, 10357815, 9768361, 17335005, 12887331
Q05655-1 (PRKCD) T507 HPRD   neXtProt   Signor 9748166, 20058876, 11381116, ...19664995, 18669648, 11781095
Q13153-1 (PAK1) T423 HPRD   neXtProt   PhosphoSitePlus 10995762
Q15349 (RPS6KA2) S218 neXtProt 10480933
Q15418 (RPS6KA1) S221 HPRD   neXtProt 10480933, 18767875, 20058876, ...9430688
Q15418-2 (RPS6KA1) S230 HPRD 10480933, 18767875, 20058876, ...9430688
Q16512 (PKN1) S773 PhosphoSitePlus 10792047
Q16512 (PKN1) T774 HPRD   neXtProt   PhosphoSitePlus   Signor 10753910, 10792047, 18669648
Q16512-2 (PKN1) T780 HPRD 10792047, 18669648
Q16513-1 (PKN2) T816 HPRD   neXtProt   PhosphoSitePlus   Signor 10753910, 10764742, 18669648
Q16513-1 (PKN2) T951 HPRD 10753910
Q96BR1 (SGK3) T320 HPRD   PhosphoSitePlus   Signor 10548550, 16790420
Q96BR1-2 (SGK3) S454 HPRD 10548550
Q96BR1-1 (SGK3) S486 HPRD 10548550
Q99683 (MAP3K5) S966 neXtProt   PhosphoSitePlus 19920149
Q99814 (EPAS1) T324 PhosphoSitePlus 17024177
Q9HBY8-2 (SGK2) T193 HPRD 10548550
Q9HBY8-2 (SGK2) S356 HPRD 10548550
Q9UBS0 (RPS6KB2) T228 HPRD   Signor 15209375, 9445476, 11733037
Q9UBS0-1 (RPS6KB2) S370 HPRD 11733037
Q9UBS0-1 (RPS6KB2) T388 HPRD 11733037
Q9Y243 (AKT3) T305 HPRD   PhosphoSitePlus   Signor 12162751, 9512493


PTM-dependent PPI

PTM type Substrate Site Interactant Association type Source PMID
Phosphorylation O15530 (PDPK1) Y9 P12931 (SRC) unknown eFIP 18024423
Phosphorylation P31749 (AKT1) T308 O15530 (PDPK1) unknown eFIP 19402821


PTM sites affected in variants

Site Variant Source PMID Disease [Sample source]
S64 F64 Biomuta DOID:4362 / cervical cancer [ cosmic, icgc ]
S92 F92 Biomuta DOID:4159 / skin cancer [ cosmic ]
Y248 H248 Biomuta DOID:363 / uterine cancer [ cosmic, icgc ]
S250 Y250 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
S381 N381 Biomuta DOID:5041 / esophageal cancer [ cosmic ]
T386 S386 Biomuta DOID:363 / uterine cancer [ cosmic, icgc ]
S390 L390 Biomuta DOID:363 / uterine cancer [ cosmic, icgc ] DOID:4159 / skin cancer [ cosmic ] DOID:1781 / thyroid gland cancer [ cosmic ] DOID:11054 / urinary bladder cancer [ cosmic ]
S394 L394 Biomuta DOID:1324 / lung cancer [ cosmic ]
S398 L398 Biomuta DOID:4606 / bile duct cancer [ cosmic ]
S400 Y400 Biomuta DOID:1612 / breast cancer [ cosmic, icgc ]
T402 K402 Biomuta DOID:4159 / skin cancer [ cosmic ]
T513 S513 Biomuta DOID:363 / uterine cancer [ cosmic, icgc ]
S549 N549 Biomuta DOID:9256 / colorectal cancer [ cosmic ]