PMID: 36255272 RLIMS-P 2 Check other iTextMine results Issue Report
Title
| 1. | The OPEN STOMATA1-SPIRAL1 module regulates microtubule stability during abscisic acid-induced stomatal closure in Arabidopsis. |
Abstract
| 2. | Drought stress triggers abscisic acid (ABA) signaling in guard cells and induces stomatal closure to prevent water loss in land plants. |
| 3. | Stomatal movement is accompanied by reorganization of the cytoskeleton. |
| 4. | Cortical microtubules disassemble in response to ABA, which is required for stomatal closure. |
| 5. | However, how ABA signaling regulates microtubule disassembly is unclear, and the microtubule-associated proteins (MAPs) involved in this process remain to be identified. |
| 6. | In this study, we show that OPEN STOMATA 1 (OST1), a central component in ABA signaling, mediates microtubule disassembly during ABA-induced stomatal closure in Arabidopsis thaliana. |
| 7. | We identified the MAP SPIRAL1 (SPR1) as the substrate of OST1. |
| 8. | OST1 interacts with and phosphorylates SPR1 at Ser6, which promotes the disassociation of SPR1 from microtubules and facilitates microtubule disassembly. |
| 9. | Compared to the wild type, the spr1 mutant exhibited significantly greater water loss and reduced ABA responses, including stomatal closure and microtubule disassembly in guard cells. |
| 10. | These phenotypes were restored by introducing the phosphorylated active form of SPR1. |
| 11. | Our findings demonstrate that SPR1 positively regulate microtubule disassembly during ABA-induced stomatal closure, which depends on OST1-mediated phosphorylation. |
| 12. | These findings reveal a specific connection between a core component of ABA signaling and MAPs. |
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