PMID: 29735970 RLIMS-P 1 Check other iTextMine results Issue Report
Title
| 1. | Jinfu'an Decoction Inhibits Invasion and Metastasis in Human Lung Cancer Cells (H1650) via p120ctn-Mediated Induction and Kaiso. |
Abstract
| 2. | BACKGROUND Previous research showed that Jin-Fu-An decoction has a significant effect on lung cancer. |
| 3. | However, it remains unclear whether p120ctn and its transcription factor Kaiso play a role in lung cancer cell proliferation, adhesion, migration, and metastasis. |
| 4. | MATERIAL AND METHODS Proliferation inhibition was detected by CCK-8 assay. |
| 5. | The migration and invasion were detected using Transwell assay. |
| 6. | The location and expression of p120ctn and Kaiso were monitored by immunofluorescence staining. |
| 7. | The expression changes of p120ctn, its isoform 1A, its S288 phosphorylation, and Kaiso were measured by Western blot assay. |
| 8. | RESULTS The lung cancer cell line H1650 administered Jin-Fu-An decoction had significantly reduced the growth in dose-dependent and time-dependent manners. |
| 9. | Migration and metastasis were significantly inhibited by application of Jin-Fu-An decoction in a dose-dependent manner. |
| 10. | Additionally, Jin-Fu-An decoction decreased the expressions of p120ctn, its isoform 1A, and its S288 phosphorylation, but the protein level of Kaiso was elevated. |
| 11. | CONCLUSIONS Jin-Fu-An decoction inhibits the proliferation, adhesion, migration, and metastasis though down-regulation of p120ctn or its isoform 1A expression, mediating the up-regulation of Kaiso. |
| 12. | The underlying mechanism of Jin-Fu-An decoction might involve targeting the lower expression of p120ctn S288 phosphorylation, which suggests that Jin-Fu-An decoction may be a potential therapeutic measure as prevention and control of recurrence and metastasis of lung cancer. |
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Legend: SUBSTRATE KINASE INTERACTANT SITE GENE MIRNA ANOMALY EXPRESSION DISEASE OUTCOME/RESPONSE SR_DRUG DRUG CELL TRIGGER Normalized
Tool: RLIMS-P
| PTM enzyme | Substrate | Site | Sentence |
|---|---|---|---|
| p120ctn (O60716) | Ser-288 | 7, 10, 12 |