PMID: 23136906    RLIMS-P  7    Check other iTextMine results Issue Report


Title
1.PKC-mediated USP phosphorylation at Ser35 modulates 20-hydroxyecdysone signaling in Drosophila.
Abstract
3.Our previous studies in Drosophila suggest that PKC modulates 20E signaling by phosphorylating EcR-USP.
4.However, the exact phosphorylation sites in EcR and USP have not been identified.
5.Using LC-MS/MS analysis, we first identified Ser35 of USP as a PKC phosphorylation site.
6.Mutation of USP Ser35 to Ala35 in S2 cells not only eliminated USP phosphorylation, but also attenuated the 20E-induced luciferase activity, mimicking the treatment with a PKC-specific inhibitor chelerythrine chloride in Kc cells.
7.In the larval salivary glands (SG), inhibition of PKC activity with the binary GAL4/UAS system reduced USP phosphorylation and down-regulated the 20E primary-response genes, E75B and Br-C, and RNAi knockdown of Rack1 had stronger inhibitory effects than overexpression of PKCi.
8.Moreover, RNAi knockdown of four PKC isozyme genes expressed in the SG exhibited a variety of inhibitory effects on USP phosphorylation and expression of E75B and Br-C, with the strongest inhibitory effects occurring when aPKC was knocked down by RNAi.
9.Taken together, we conclude that PKC-mediated USP phosphorylation at Ser35 modulates 20E signaling in Drosophila.
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Legend:   SUBSTRATE  KINASE  INTERACTANT  SITE  GENE  MIRNA  ANOMALY  EXPRESSION  DISEASE  OUTCOME/RESPONSE  SR_DRUG  DRUG  CELL  TRIGGER  Normalized

Tool: RLIMS-P

PTM enzymeSubstrateSiteSentence
USP (P20153)Ser-351, 6, 9
PKC (P05130)EcR (P34021), USP (P20153)Ser-354, 5
USP (P20153)4, 7
EcR (P34021)4
USP (P20153)Br-C8
PKC (P05130)EcR-USP (P34021)3
USP (P20153)E75B (P17672)8