PMID: 15225637 RLIMS-P 3 Check other iTextMine results Issue Report
Title
| 1. | Phosphorylation of the regulatory beta-subunit of protein kinase CK2 by checkpoint kinase Chk1: identification of the in vitro CK2beta phosphorylation site. |
Abstract
| 2. | The regulatory beta-subunit of protein kinase CK2 mediates the formation of the CK2 tetrameric form and it has functions independent of CK2 catalytic subunit through interaction with several intracellular proteins. |
| 3. | Recently, we have shown that CK2beta associates with the human checkpoint kinase Chk1. |
| 4. | In this study, we show that Chk1 specifically phosphorylates in vitro the regulatory beta-subunit of CK2. |
| 5. | Chymotryptic peptides and mutational analyses have revealed that CK2beta is phosphorylated at Thr213. |
| 6. | Formation of a stable complex between CK2beta and Chk1 is not affected by the modification of Thr213 but it does require the presence of an active Chk1 kinase. |
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