PMID: 26985018 RLIMS-P 1 eFIP 0 miRTex 0 eGARD 0 Issue Report
Title
| 1. | Tension on JAM-A activates RhoA via GEF-H1 and p115 RhoGEF. |
Abstract
| 2. | Junctional adhesion molecule A (JAM-A) is a broadly expressed adhesion molecule that regulates cell-cell contacts and facilitates leukocyte transendothelial migration. |
| 3. | The latter occurs through interactions with the integrin LFA-1. |
| 4. | Although we understand much about JAM-A, little is known regarding the protein's role in mechanotransduction or as a modulator of RhoA signaling. |
| 5. | We found that tension imposed on JAM-A activates RhoA, which leads to increased cell stiffness. |
| 6. | Activation of RhoA in this system depends on PI3K-mediated activation of GEF-H1 and p115 RhoGEF. |
| 7. | These two GEFs are further regulated by FAK/ERK and Src family kinases, respectively. |
| 8. | Finally, we show that phosphorylation of JAM-A at Ser-284 is required for RhoA activation in response to tension. |
| 9. | These data demonstrate a direct role of JAM-A in mechanosignaling and control of RhoA and implicate Src family kinases in the regulation of p115 RhoGEF. |
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Legend: SUBSTRATE KINASE INTERACTANT SITE GENE MIRNA ANOMALY EXPRESSION DISEASE OUTCOME/RESPONSE SR_DRUG DRUG CELL TRIGGER Normalized
Tool: RLIMS-P
| PTM enzyme | Substrate | Site | Sentence |
|---|---|---|---|
| JAM-A (Q9Y624) | Ser-284 | 8 |