TI - Note : . AB - After these data were obtained , two studies have reported AlkB -mediated repair of 3-meT ( 35,36 ) and 1-meG ( 35 ) lesions , using experimental strategies rather different from those used here . The reported data are consistent with those obtained in the present study . Koivisto et al. ( 36 ) reported oxidative demethylation by AlkB , hABH2 and hABH3 of 3-meT lesions induced by treatment of poly ( dT ) with methylating agents . Delaney and Essigmann ( 35 ) used single-stranded DNA subSTRates containing a single 1-meG or 3-meT lesion to study the mutagenicity and cytotoxicity of these lesions in wild-type and alkB mutant E.coli , and their results indicated that both 1-meG and 3-meT are AlkB SUBstrates in vivo .