TI - Conclusion . AB - In summary , we have shown that a novel variant of ER-alpha66 , ER-alpha36 is localized on the plasma membrane of endometrial cancer Hec1A cells . We demonstrated that testosterone induces ERK and Akt PHOSphorylation via ER-alpha36 -mediated membrane-initiated pathways . The present study thus shed new light on understanding testosterone-stimulated endometrial carcinogenesis . Further research of ER-alpha36 functions may provide novel information for designing new drugs for the treatment of endometrial cancer .