TI - IL6ST contributes to AblPP -induced cell detachment . AB - Based on the pathway analysis of the shRNA screening results , we decided to validate the IL6ST out of the candidate genes , because it has been shown to regulate cell cell adhesion in cultured cardiomyocytes [33] . To validate the function of IL6ST in our study , we used new shRNA sequences and infected AblPP cells with a lentiviral vector to express these shRNAs ( see Methods for details ) . Quantitative PCR results indicated that the mRNA level of IL6ST was significantly reduced ( Fig 3A ) . The cells depleted of IL6ST were resistant to AblPP -induced cell detachment (Fig 3B) , and this phenotype was not due to either a loss of AblPP expression or a loss of PHOSphorylation in AblPP cells (Fig 3C) , suggesting that IL6ST is a bona fide effector in Abl kinase -induced cell detachment pathway .