TI - Up-regulation of SOCS3 inhibited cell migration and associated PYK2 expression , Tyr402 and ERK1/2 PHOSphorylations in A549 cells . AB - To investigate whether SOCS3 involves in regulating cell migration , the number of migrated A549 cells with 5-aza-2'-deoxycytidine treatment or not was evaluated . The numbers of migrated cells with or without 5-aza-2'-deoxycytidine treated and with beta-lactacystin pretreatment were 9.2 + - 3.4 , 16.9 + - 3.6 and 13.4 + - 3.3 , respectively ( p lt 005 , Fig 4c ) . We also identified that PYK2 expression , Tyr402 and ERK1/2 phosphorylaTIONs were suppressed upon SOCS3 up-regulation by 5-aza-2'-deoxycytidine treatment ( p lt 005 , Fig 4a ) and the decreased Tyr402 PHOSphorylation was in parallel with the reduced PYK2 expression which was possibly attributed to the process of proteolytic degradation , because elevated PYK2 expression was detected in cells pretreated with the proteasome inhibitor beta-lactacystin ( p lt 005 , Fig 4a ) , and PYK2 mRNA levels were unaffected ( p gt 005 , Fig 4b ) .