TI - FZC18 suppresses Wnt/beta-catenin signaling . AB - FZC18 suppressed CRT and total and non-PHOSphorylated beta-catenin stabilization in a dose -dependent manner ( Figure 5 , A and B ) . In addition , FZC18 downregulated cyclin D1 promoter activity ( Figure 5C ) and decreased colony formation ( Figure 5D ) by 75% compared with cells expressing vector alone . In contrast , V2Nter induced a 21% increase in colony formation with respect to empty vector ( Figure 5D ) , consistently with data on CRT ( Figures 2G and S1F ) and cyclin D1 promoter activity (Figure S1I) .