TI - c-Abl is required for oxidative stress-induced phosphorylation of caveolin-1 on tyrosine 14 . AB - Caveolin-1 is phosphorylated at tyrosine 14 in response to cellular stress . Tyrosine 14 is a consensus Abl phosphorylation site suggesting that caveolin-1 may be an Abl substrate . We report here that expression of c-Abl is required for oxidative stress-induced caveolin-1 phosphorylation . In contrast , c-Src expression is not required . Phosphocaveolin is one of only two phosphotyrosine signals missing in lysates from the Abl(-/-) cells , indicating that these cells still respond to oxidative stress . Oxidative stress-induced tyrosine phosphorylation of caveolin-1 occurs only at the Abl site , tyrosine 14 . Caveolin-1 is also a major phosphotyrosine signal detected in cells over-expressing c-Abl . Our results show that Abl activation leads to phosphorylation of caveolin-1 on tyrosine 14 . Both Abl and caveolin have been linked to the actin cytoskeleton , and oxidative stress-induced phosphocaveolin is enriched at focal contacts . This suggests that phosphocaveolin regulates these structures , perhaps through recruiting and activating SH2-domain proteins such as Csk .