Increased levels of microRNA-424 are associated with the pathogenesis of fetal growth restriction. Studies show a number of miRNAs are expressed in the placenta including miRNA-424, a hypoxia-regulated miRNA, suggesting miRNA-424 may have a potential regulatory role in fetal growth restriction (FGR). The levels of miRNA-424 and its target gene, mitogen-activated protein kinase 1 (MEK1), and fibroblast growth factor receptor 1 (FGFR1) were examined. The levels of miRNA-424 were significantly increased in placentae from women with FGR . Both mRNA levels and protein levels of MEK1 and FGFR1 were significantly reduced in placentae complicated with FGR . Furthermore, the increased levels of miRNA-424 were negatively correlated with the mRNA levels of MEK1 in placentae complicated with FGR . Our study suggests upregulated miRNA-424 may be associated with the pathogenesis of FGR .