Regulation of miR-200c by nuclear receptors PPARalpha, LRH-1 and SHP . We investigated regulation of miR-200c expression by nuclear receptors. Ectopic expression of miR-200c inhibited MHCC97H cell migration, which was abrogated by the synergistic effects of PPARalpha and LRH-1 siRNAs. The expression of miR-200c was decreased by PPARalpha/LRH-1 siRNAs and increased by SHP siRNAs, and overexpression of the receptors reversed the effects of their respective siRNAs. SHP siRNAs also drastically enhanced the ability of the LRH-1 agonist RJW100 to induce miR-200c and downregulate ZEB1 and ZEB2 proteins . Co-expression of PPARalpha and LRH-1 moderately transactivated the miR-200c promoter, which was repressed by SHP co-expression. RJW100 caused strong activation of the miR-200c promoter . This is the first report to demonstrate that miR-200c expression is controlled by nuclear receptors.