iPTMnet Report for Q8NB16 (MLKL)


Protein Information

Cytoscape View


UniProt AC / UniProt ID Q8NB16 / MLKL_HUMAN
Protein Name Mixed lineage kinase domain-like protein
Gene Name Name: MLKL
Organism Homo sapiens (Human)

PRO ID PR:Q8NB16
DAG View
PRO Name mixed lineage kinase domain-like protein (human)
Definition A mixed lineage kinase domain-like protein that is encoded in the genome of human.
Short Label hMLKL
Category organism-gene

Interactive Sequence View

Select/align proteoforms across species


Q8NB16 (MLKL) as Substrate



Site PTM Type PTM Enzyme Score Source PMID
K40 Ubiquitination PhosphoSitePlus
K50 Ubiquitination PhosphoSitePlus
S52 Phosphorylation PhosphoSitePlus
K57 Ubiquitination PhosphoSitePlus
T59 Phosphorylation PhosphoSitePlus
K66 Ubiquitination PhosphoSitePlus
K78 Ubiquitination PhosphoSitePlus
C86 Methylation IEDB 31844290
S92 Phosphorylation PhosphoSitePlus
S106 Phosphorylation PhosphoSitePlus
S125 Phosphorylation phospho.ELM   PhosphoSitePlus   UniProt 18691976, 18669648
S128 Phosphorylation PhosphoSitePlus
K157 Ubiquitination PhosphoSitePlus
S161 Phosphorylation PhosphoSitePlus
K173 Ubiquitination PhosphoSitePlus
K183 Ubiquitination PhosphoSitePlus
K198 Ubiquitination PhosphoSitePlus
K219 Ubiquitination PhosphoSitePlus
K230 Ubiquitination PhosphoSitePlus
T246 Phosphorylation PhosphoSitePlus
K249 Ubiquitination PhosphoSitePlus
T253 O-Glycosylation GlyGen 29237092
S259 O-Glycosylation GlyGen 29237092
T302 Phosphorylation PhosphoSitePlus
K331 Ubiquitination PhosphoSitePlus
S334 Phosphorylation PhosphoSitePlus
T340 Phosphorylation IEDB 32387766
K354 Acetylation PhosphoSitePlus
K354 Ubiquitination PhosphoSitePlus
T357 O-Glycosylation GlyGen 29237092
T357 Phosphorylation Q9Y572 (RIPK3) PhosphoSitePlus   RLIMS-P   UniProt 24703947, 31839993, 26184911, ...22265413, 31727943
S358 O-Glycosylation GlyGen 29237092
S358 Phosphorylation Q9Y572 (RIPK3) PhosphoSitePlus   RLIMS-P   UniProt 29104146, 24901049, 31914150, ...30157175, 24703947, 31839993, 29883610, 26184911, 27362805, 22265413, 31727943
T364 Phosphorylation PhosphoSitePlus
K372 Ubiquitination PhosphoSitePlus
S373 Phosphorylation PhosphoSitePlus
T374 Phosphorylation phospho.ELM   PhosphoSitePlus 18669648
Y376 Phosphorylation PhosphoSitePlus
S393 Phosphorylation PhosphoSitePlus
S417 Phosphorylation PhosphoSitePlus
S467 Phosphorylation PhosphoSitePlus
Site PTM Type PTM Enzyme Score Source PMID
S125 Phosphorylation HPRD 18691976, 18669648, 20068231
T374 Phosphorylation HPRD 18669648


PTM-dependent PPI

PTM type Substrate Site Interactant Association type Source PMID
Phosphorylation Q9Y572 (RIPK3) S227 Q8NB16 (MLKL) unknown eFIP 26866270
Phosphorylation Q9Y572 (RIPK3) S227 Q8NB16 (MLKL) unknown eFIP 23612963


PTM sites affected in variants

Site Variant Source PMID Disease [Sample source]
K40 E40 Biomuta DOID:11054 / urinary bladder cancer [ cosmic, icgc ]
S52 N52 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
C86 F86 Biomuta DOID:184 / bone cancer [ cosmic ]
S92 N92 Biomuta DOID:9256 / colorectal cancer [ cosmic, icgc ]
S106 N106 Biomuta DOID:363 / uterine cancer [ icgc ]
S161 L161 Biomuta DOID:363 / uterine cancer [ cosmic, icgc ]
K230 Q230 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
T246 S246 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
T253 N253 Biomuta DOID:1612 / breast cancer [ cosmic ]
T340 I340 Biomuta DOID:363 / uterine cancer [ cosmic, icgc ]
T364 M364 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
T364 K364 Biomuta [ cosmic ]
S373 C373 Biomuta DOID:11054 / urinary bladder cancer [ cosmic, icgc ]
T374 R374 Biomuta DOID:10534 / stomach cancer [ cosmic ]
S467 F467 Biomuta DOID:3571 / liver cancer [ cosmic ]