iPTMnet Report for P15056 (BRAF)


Protein Information

Cytoscape View


UniProt AC / UniProt ID P15056 / BRAF_HUMAN
Protein Name Serine/threonine-protein kinase B-raf
Gene Name Name: BRAF
Synonyms:BRAF1,RAFB1;
Organism Homo sapiens (Human)

PRO ID PR:P15056
DAG View
PRO Name serine/threonine-protein kinase B-raf (human)
Definition A B-Raf proto-oncogene serine/threonine-protein kinase that is encoded in the genome of human.
Short Label hBRAF
Category organism-gene

Interactive Sequence View

Select/align proteoforms across species


P15056 (BRAF) as Substrate



Site PTM Type PTM Enzyme Score Source PMID
S76 Phosphorylation PhosphoSitePlus
T119 Phosphorylation HPRD   PhosphoSitePlus 16858395
S147 Phosphorylation HPRD   PhosphoSitePlus 17192257
S147 Phosphorylation P15056 (BRAF) HPRD 17192257
S151 Phosphorylation HPRD   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P+   RLIMS-P   Signor   UniProt 22292133, 23186163, 29335436, ...17192257, 27034005, 24275569, 20068231, 21478863
S151 Phosphorylation P15056 (BRAF) HPRD 17192257, 20068231
S151 Phosphorylation P27361 (MAPK3) RLIMS-P+   Signor 21478863
S151 Phosphorylation P28482 (MAPK1) RLIMS-P+   Signor 21478863
K164 Ubiquitination PhosphoSitePlus
T244 Phosphorylation PhosphoSitePlus
K253 Acetylation PhosphoSitePlus
K253 Ubiquitination PhosphoSitePlus
S314 Phosphorylation PhosphoSitePlus
S316 Phosphorylation PhosphoSitePlus
S317 Phosphorylation PhosphoSitePlus
S319 Phosphorylation HPRD   phospho.ELM   PhosphoSitePlus 18669648
S333 Phosphorylation HPRD   phospho.ELM   PhosphoSitePlus 18669648
S335 Phosphorylation HPRD   phospho.ELM   PhosphoSitePlus 18669648
S339 Phosphorylation PRO   PhosphoSitePlus
S363 Phosphorylation HPRD   PhosphoSitePlus 18691976, 20058876, 20068231
S364 Phosphorylation HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor 18451171, 18669648, 10869359, ...11410590
S364 Phosphorylation O00141 (SGK1) neXtProt 11410590
S364 Phosphorylation P31751 (AKT2) Signor 10869359
S364 Phosphorylation Q9Y243 (AKT3) Signor 10869359
S365 Phosphorylation HPRD   neXtProt   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P   Signor   UniProt 18451171, 10869359, 23186163, ...18220336, 20230923, 20058876, 17192257, 18452278, 19651622, 18669648, 31453322, 11410590, 20068231
S365 Phosphorylation O00141 (SGK1) HPRD 10869359, 20230923, 20058876, ...18452278, 19651622, 18669648, 11410590, 20068231
S365 Phosphorylation P31749 (AKT1) HPRD   neXtProt   PhosphoSitePlus   Signor 10869359, 20230923, 20058876, ...18452278, 19651622, 18669648, 11410590, 20068231
S365 Phosphorylation Q9Y243 (AKT3) neXtProt   PhosphoSitePlus 18451171
T373 Phosphorylation HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   UniProt 20230923, 18220336, 17192257, ...1508179, 20068231
T373 Phosphorylation P15056 (BRAF) HPRD   neXtProt   PhosphoSitePlus   UniProt 1508179, 17192257, 20068231, ...20230923
S394 Phosphorylation PhosphoSitePlus
T396 Phosphorylation PhosphoSitePlus
S399 Phosphorylation phospho.ELM   PhosphoSitePlus 15302935
T401 Phosphorylation HPRD   neXtProt   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P   UniProt 23186163, 16964243, 15302935, ...17192257, 18669648, 27034005, 21135229, 26898828, 20068231, 29335436
T401 Phosphorylation P27361 (MAPK3) neXtProt   PhosphoSitePlus 21135229
T401 Phosphorylation P28482 (MAPK1) PhosphoSitePlus 21135229
S405 Phosphorylation PhosphoSitePlus   RLIMS-P 26898828
K418 Acetylation PhosphoSitePlus
S419 Phosphorylation HPRD   phospho.ELM   PhosphoSitePlus   RLIMS-P 27034005, 17192257, 17924679
S419 Phosphorylation P15056 (BRAF) HPRD 17192257, 17924679
S428 Phosphorylation PhosphoSitePlus   RLIMS-P   Signor 18451171, 10869359
S428 Phosphorylation P31751 (AKT2) Signor 10869359
S428 Phosphorylation Q9Y243 (AKT3) Signor 10869359
S429 Phosphorylation HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   Signor 18451171, 10869359, 11510412
S429 Phosphorylation P31749 (AKT1) HPRD   neXtProt   PhosphoSitePlus   Signor 10869359
S429 Phosphorylation Q9Y243 (AKT3) neXtProt   PhosphoSitePlus 18451171
S430 Phosphorylation HPRD   PhosphoSitePlus 11510412
S430 Phosphorylation P17612 (PRKACA) HPRD 11510412
S431 Phosphorylation PhosphoSitePlus
S432 Phosphorylation PhosphoSitePlus
T440 Phosphorylation HPRD   phospho.ELM   PhosphoSitePlus   Signor 10869359
T440 Phosphorylation P31751 (AKT2) Signor 10869359
S446 Phosphorylation HPRD   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P   UniProt 16702958, 23186163, 15710605, ...18691976, 32782246, 17192257, 18452278, 19651622, 18669648, 16803888, 20166139, 20068231
S446 Phosphorylation Q13153 (PAK1) phospho.ELM   PhosphoSitePlus 15710605
S446 Phosphorylation Q15382 (RHEB) HPRD 18691976, 18452278, 19651622, ...18669648, 16803888, 20166139, 20068231
S447 Phosphorylation HPRD   phospho.ELM   PhosphoSitePlus   RLIMS-P   UniProt 32782246, 20058876, 19651622, ...18669648, 20068231
T458 Phosphorylation PhosphoSitePlus
S465 Phosphorylation PhosphoSitePlus   RLIMS-P 27034005
S467 Phosphorylation PhosphoSitePlus
T470 Phosphorylation PhosphoSitePlus
K473 Ubiquitination PhosphoSitePlus
K475 Ubiquitination PhosphoSitePlus
Y566 Phosphorylation PhosphoSitePlus
K578 Ubiquitination PhosphoSitePlus   UniProt 23907581
S579 Phosphorylation phospho.ELM   PhosphoSitePlus 16093354
T599 Phosphorylation HPRD   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P 26744778, 22506009, 29844492, ...23907581, 11032810, 15258589
K601 Acetylation PhosphoSitePlus
K601 Ubiquitination PhosphoSitePlus
S602 Phosphorylation HPRD   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P 26744778, 29844492, 23907581, ...11032810, 15258589
S605 Phosphorylation IEDB   PhosphoSitePlus   RLIMS-P 27034005, 11120772
S614 Phosphorylation PhosphoSitePlus   RLIMS-P+ 27345148
S614 Phosphorylation P15056 (BRAF) RLIMS-P+ 27345148
R671 Methylation UniProt 21917714
S675 Phosphorylation PhosphoSitePlus
K687 Ubiquitination PhosphoSitePlus
S727 Phosphorylation phospho.ELM 15302935
S729 Phosphorylation HPRD   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P   UniProt 18691976, 21406692, 23186163, ...15302935, 32782246, 17192257, 33375360, 19651622, 18669648, 20068231, 24095280, 20166139, 31453322, 27399332
S729 Phosphorylation Q13131 (PRKAA1) PhosphoSitePlus 24095280
S732 Phosphorylation PhosphoSitePlus
Y746 Phosphorylation PhosphoSitePlus
S750 Phosphorylation HPRD   neXtProt   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P 29335436, 17192257, 14654779
S750 Phosphorylation P15056 (BRAF) HPRD 17192257
S750 Phosphorylation P28482 (MAPK1) neXtProt 14654779
T753 Phosphorylation neXtProt   PRO   PhosphoSitePlus   RLIMS-P   Signor   UniProt 19710016, 29335436, 14654779, ...16508002, 19933846
T753 Phosphorylation P27361 (MAPK3) PhosphoSitePlus   Signor 19933846, 16508002
T753 Phosphorylation P28482 (MAPK1) neXtProt   PhosphoSitePlus 16508002, 14654779
Y760 Phosphorylation PhosphoSitePlus


P15056 (BRAF) as PTM Enzyme



Substrate Site Score Source PMID
P15056 (BRAF) S147 HPRD 17192257
P15056 (BRAF) S151 HPRD 17192257, 20068231
P15056 (BRAF) T373 HPRD   neXtProt   PhosphoSitePlus   UniProt 1508179, 17192257, 20068231, ...20230923
P15056 (BRAF) S419 HPRD 17192257, 17924679
P15056 (BRAF) S614 RLIMS-P+ 27345148
P15056 (BRAF) S750 HPRD 17192257
P33981 (TTK) S281 PhosphoSitePlus 22430208
P33981 (TTK) S436 PhosphoSitePlus 22430208
P36507 (MAP2K2) S222 neXtProt   PhosphoSitePlus   Signor 17667937, 17210721, 8668348
P36507 (MAP2K2) S226 neXtProt   PhosphoSitePlus   Signor 17667937, 17210721, 8668348
P68104 (EEF1A1) S21 PhosphoSitePlus 22378069
P68104 (EEF1A1) T88 PhosphoSitePlus 22378069
Q02750 (MAP2K1) S218 HPRD   neXtProt   PhosphoSitePlus 17667937, 17210721, 8394352, ...7731720, 8157000, 8621729
Q02750-1 (MAP2K1) S222 HPRD   neXtProt   PhosphoSitePlus 17667937, 17210721, 8394352, ...7731720, 8157000, 8621729
Q05639 (EEF1A2) S21 PhosphoSitePlus 22378069
Q92934 (BAD) S75 neXtProt   PhosphoSitePlus 19667065
Q92934 (BAD) S99 neXtProt   PhosphoSitePlus 19667065
Q92934 (BAD) S118 neXtProt   PhosphoSitePlus 19667065
Q92934 (BAD) S134 PhosphoSitePlus 21317286


Proteoforms

PRO ID (Short Label) Sites PTM Enzyme Source PMID
PR:000046506 (hBRAF/InitMet-/Phos:2) pS151,pT401,pS446,pT599,pS602,pS729,pS750,pT753 PRO
PR:000046507 (hBRAF/InitMet-/Phos:3) pS339 PRO
PR:000046508 (hBRAF/InitMet-/Phos:4) pS365,pS446,pS729 PRO
PR:000046509 (hBRAF/InitMet-/Phos:5) pS446,pT599,pS602,pS729 PRO
PR:000046510 (hBRAF/InitMet-/Phos:6) pS446,pS729 PRO


PTM-dependent PPI

PTM type Substrate Site Interactant Association type Source PMID
Phosphorylation P12931 (SRC) S17 P15056 (BRAF) unknown eFIP 12221082
Phosphorylation P15056 (BRAF) S729 Q8IVT5 (KSR1) inhibited_association eFIP 24095280
Phosphorylation Q16584 (MAP3K11) S705 P15056 (BRAF) unknown eFIP 29084209


Proteoform PPIs

Protein 1 Relation Protein 2 Source PMID
PR:000044747 (mBAD/iso:1/UnMod) Interaction PR:P15056 (hBRAF) PRO 17011751


PTM sites affected in variants

Site Variant Source PMID Disease [Sample source]
T119 S119 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
S147 N147 Biomuta DOID:10283 / prostate cancer [ cosmic ]
S151 A151 Biomuta DOID:10283 / prostate cancer [ cosmic ]
T244 I244 Biomuta DOID:4159 / skin cancer [ cosmic ]
S316 P316 Biomuta DOID:11054 / urinary bladder cancer [ cosmic ]
S317 F317 Biomuta DOID:363 / uterine cancer [ cosmic ]
S333 G333 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
S339 F339 Biomuta DOID:4159 / skin cancer [ cosmic ]
S363 F363 Biomuta DOID:4159 / skin cancer [ cosmic ]
S364 L364 Biomuta DOID:11054 / urinary bladder cancer [ cosmic ]
S365 L365 Biomuta DOID:4159 / skin cancer [ cosmic ]
S394 T394 Biomuta DOID:2531 / hematologic cancer [ cosmic ]
S394 * Biomuta DOID:1324 / lung cancer [ cosmic ]
T401 I401 Biomuta DOID:4362 / cervical cancer [ cosmic ]
T401 A401 Biomuta DOID:10283 / prostate cancer [ cosmic ]
S419 Y419 Biomuta DOID:363 / uterine cancer [ cosmic ]
S428 * Biomuta DOID:363 / uterine cancer [ cosmic ]
S428 T428 Biomuta DOID:363 / uterine cancer [ cosmic ]
S428 L428 Biomuta DOID:10283 / prostate cancer [ cosmic ]
T440 P440 Biomuta DOID:1324 / lung cancer [ cosmic ]
S446 L446 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
S447 N447 Biomuta DOID:10534 / stomach cancer [ cosmic ]
S447 C447 Biomuta DOID:10534 / stomach cancer [ cosmic ]
S465 F465 Biomuta [ cosmic ] DOID:9256 / colorectal cancer [ cosmic ]
S467 L467 Biomuta [ cosmic ] DOID:4159 / skin cancer [ cosmic ] DOID:1324 / lung cancer [ cosmic ]
K473 M473 Biomuta DOID:1612 / breast cancer [ cosmic ]
K475 M475 Biomuta DOID:4159 / skin cancer [ cosmic ]
Y566 C566 Biomuta DOID:4159 / skin cancer [ cosmic ]
S579 G579 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
T599 I599 Biomuta DOID:4159 / skin cancer [ cosmic ] DOID:9256 / colorectal cancer [ cosmic ] DOID:1324 / lung cancer [ cosmic ] DOID:11054 / urinary bladder cancer [ cosmic ]
T599 R599 Biomuta DOID:9256 / colorectal cancer [ cosmic ] DOID:1781 / thyroid gland cancer [ cosmic ]
K601 T601 Biomuta [ cosmic ] DOID:1793 / pancreatic cancer [ cosmic ] DOID:10534 / stomach cancer [ cosmic ] DOID:2531 / hematologic cancer [ cosmic ]
K601 N601 Biomuta [ cosmic ] DOID:10283 / prostate cancer [ cosmic ] DOID:1324 / lung cancer [ cosmic ] DOID:2531 / hematologic cancer [ cosmic ] DOID:1612 / breast cancer [ cosmic ] DOID:4159 / skin cancer [ cosmic ] DOID:2394 / ovarian cancer [ cosmic ] DOID:9256 / colorectal cancer [ cosmic ] DOID:4606 / bile duct cancer [ cosmic ]
K601 E601 Biomuta [ cosmic ] DOID:10283 / prostate cancer [ cosmic ] DOID:1324 / lung cancer [ cosmic ] DOID:2531 / hematologic cancer [ cosmic ] DOID:1612 / breast cancer [ cosmic ] DOID:363 / uterine cancer [ cosmic ] DOID:2394 / ovarian cancer [ cosmic ] DOID:9256 / colorectal cancer [ cosmic ] DOID:4159 / skin cancer [ cosmic ] DOID:10534 / stomach cancer [ cosmic ] DOID:1781 / thyroid gland cancer [ cosmic ] DOID:8618 / oral cavity cancer [ cosmic ] DOID:9253 / gastrointestinal stromal tumor [ cosmic ] DOID:4606 / bile duct cancer [ cosmic ]
K601 I601 Biomuta DOID:4159 / skin cancer [ cosmic ]
S602 Y602 Biomuta DOID:363 / uterine cancer [ cosmic ] DOID:9256 / colorectal cancer [ cosmic ]
S605 N605 Biomuta DOID:4159 / skin cancer [ cosmic ]
S605 G605 Biomuta DOID:4159 / skin cancer [ cosmic ]
S605 R605 Biomuta DOID:4159 / skin cancer [ cosmic ]
S614 P614 Biomuta DOID:4159 / skin cancer [ cosmic ] DOID:1319 / brain cancer [ cosmic ]
S614 Y614 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
R671 Q671 Biomuta [ cosmic ] DOID:363 / uterine cancer [ cosmic ] DOID:4159 / skin cancer [ cosmic ]
R671 * Biomuta DOID:10534 / stomach cancer [ cosmic ]
R671 L671 Biomuta DOID:1324 / lung cancer [ cosmic ]
S727 T727 Biomuta DOID:1324 / lung cancer [ cosmic ]
S727 I727 Biomuta DOID:4159 / skin cancer [ cosmic ]
S727 G727 Biomuta DOID:1793 / pancreatic cancer [ cosmic ]
S732 F732 Biomuta DOID:4159 / skin cancer [ cosmic ]
Y760 C760 Biomuta DOID:5041 / esophageal cancer [ cosmic ]