iPTMnet Report for P04406 (GAPDH)


Protein Information

Cytoscape View


UniProt AC / UniProt ID P04406 / G3P_HUMAN
Protein Name Glyceraldehyde-3-phosphate dehydrogenase
Gene Name Name: GAPDH
Synonyms:GAPD;ORFNames:CDABP0047,OK/SW-cl.12;
Organism Homo sapiens (Human)

PRO ID PR:P04406
DAG View
PRO Name glyceraldehyde-3-phosphate dehydrogenase (human)
Definition A glyceraldehyde-3-phosphate dehydrogenase that is encoded in the genome of human.
Short Label hGAPDH
Category organism-gene

Interactive Sequence View

Select/align proteoforms across species


P04406 (GAPDH) as Substrate



Site PTM Type PTM Enzyme Score Source PMID
K5 Acetylation PhosphoSitePlus
K5 Methylation PhosphoSitePlus   UniProt 18183946
K5 Ubiquitination PhosphoSitePlus
R13 Methylation PhosphoSitePlus
S25 Phosphorylation PhosphoSitePlus
K27 Acetylation PhosphoSitePlus
K27 Ubiquitination PhosphoSitePlus
Y42 Phosphorylation P12931 (SRC) PhosphoSitePlus   UniProt 15592455, 17488287
Y45 Phosphorylation PhosphoSitePlus
Y49 Phosphorylation PhosphoSitePlus
S51 Phosphorylation PhosphoSitePlus
T52 Phosphorylation PhosphoSitePlus
K55 Acetylation PhosphoSitePlus
K55 Ubiquitination PhosphoSitePlus
T59 Phosphorylation PhosphoSitePlus
K61 Acetylation PhosphoSitePlus   UniProt 19608861
K61 Sumoylation PhosphoSitePlus
K61 Ubiquitination PhosphoSitePlus
K66 Acetylation PhosphoSitePlus
K66 Methylation PhosphoSitePlus   UniProt 18183946
K66 Ubiquitination PhosphoSitePlus
T75 Phosphorylation PhosphoSitePlus   UniProt 18183946, 20068231
R80 Methylation PhosphoSitePlus
S83 O-Glycosylation GlyGen 30444036, 35138101, 29351928, ...16408927, 34725712, 32119511, 21300897, 30397120, 34931806, 20305658, 28657654, 33214551, 28510447, 24126823, 30379171, 26374642, 18369606, 21740066, 35254053, 23576270, 34019948, 35083852, 18984734, 23332158
S83 Phosphorylation phospho.ELM   PhosphoSitePlus   UniProt 21406692, 23186163, 17081983, ...18669648, 20068231
K84 Acetylation PhosphoSitePlus
K84 Ubiquitination PhosphoSitePlus
K86 Acetylation PhosphoSitePlus
K86 Ubiquitination PhosphoSitePlus
Y94 Phosphorylation PhosphoSitePlus
S98 Phosphorylation PhosphoSitePlus
T99 Phosphorylation PhosphoSitePlus   RLIMS-P 26629320
T103 Phosphorylation PhosphoSitePlus
T104 Phosphorylation PhosphoSitePlus
K107 Acetylation PhosphoSitePlus
K107 Ubiquitination PhosphoSitePlus
K117 Acetylation PhosphoSitePlus
K117 Ubiquitination PhosphoSitePlus
S122 Phosphorylation Q13131 (PRKAA1) PhosphoSitePlus   RLIMS-P   UniProt 18183946, 26626483
S125 Phosphorylation PhosphoSitePlus
K139 Acetylation PhosphoSitePlus
K139 Ubiquitination PhosphoSitePlus
Y140 Phosphorylation PhosphoSitePlus
S143 Phosphorylation PhosphoSitePlus
K145 Acetylation PhosphoSitePlus
K145 Ubiquitination PhosphoSitePlus
S148 Phosphorylation PhosphoSitePlus   UniProt 18183946
N149 N-Glycosylation GlyGen 29391424
S151 Phosphorylation PhosphoSitePlus   UniProt 18669648, 23186163
C152 S-Nitrosylation dbSNO 25040305, 20140087
T153 Phosphorylation PhosphoSitePlus   UniProt 23186163
T154 Phosphorylation PhosphoSitePlus
C156 S-Nitrosylation dbSNO 25040305
K162 Acetylation PhosphoSitePlus
K162 Methylation PhosphoSitePlus
K162 Sumoylation PhosphoSitePlus
K162 Ubiquitination PhosphoSitePlus
T176 Phosphorylation PhosphoSitePlus
T177 Phosphorylation PhosphoSitePlus   UniProt 24275569
T182 Phosphorylation PhosphoSitePlus   UniProt 24275569
T184 Phosphorylation PhosphoSitePlus   UniProt 20068231, 18669648, 19690332, ...24275569
K186 Acetylation PhosphoSitePlus
K186 Ubiquitination PhosphoSitePlus
T187 Phosphorylation PhosphoSitePlus
S192 Phosphorylation PhosphoSitePlus
K194 Acetylation PhosphoSitePlus   UniProt 19608861
K194 Methylation PhosphoSitePlus   UniProt 18183946
K194 Sumoylation PhosphoSitePlus
K194 Ubiquitination PhosphoSitePlus
R200 Methylation PhosphoSitePlus
S210 O-Glycosylation GlyGen 30444036, 35138101, 29351928, ...16408927, 34725712, 31373491, 32119511, 21300897, 30397120, 34931806, 20305658, 28657654, 33214551, 28510447, 24126823, 30379171, 26374642, 18369606, 21740066, 35254053, 23576270, 34019948, 35083852, 18984734, 23332158
S210 Phosphorylation PhosphoSitePlus
T211 Phosphorylation PhosphoSitePlus   UniProt 19690332
K215 Acetylation PhosphoSitePlus
K215 Methylation UniProt 18183946
K215 Sumoylation PhosphoSitePlus
K215 Ubiquitination PhosphoSitePlus
K219 Acetylation PhosphoSitePlus   UniProt 19608861
K219 Methylation PhosphoSitePlus
K219 Sumoylation PhosphoSitePlus
K219 Ubiquitination PhosphoSitePlus
K227 Acetylation PhosphoSitePlus   UniProt 19608861
K227 Methylation PhosphoSitePlus   UniProt 18183946
K227 Sumoylation PhosphoSitePlus
K227 Ubiquitination PhosphoSitePlus
T229 Phosphorylation PhosphoSitePlus   UniProt 18183946, 23186163
R234 Methylation PhosphoSitePlus
T237 Phosphorylation P31751 (AKT2) PhosphoSitePlus   RLIMS-P   UniProt 18183946, 21979951
N239 N-Glycosylation GlyGen 29391424
S241 Phosphorylation PhosphoSitePlus   UniProt 24275569
T246 Phosphorylation Q05655 (PRKCD) PhosphoSitePlus   RLIMS-P 23653351
C247 Methylation IEDB 31844290
C247 S-Nitrosylation dbSNO 2212679, 22126794, 26338901, ...20140087
R248 Methylation PhosphoSitePlus
K251 Acetylation PhosphoSitePlus
K251 Ubiquitination PhosphoSitePlus
K254 Acetylation PhosphoSitePlus   UniProt 19608861
K254 Sumoylation PhosphoSitePlus
K254 Ubiquitination PhosphoSitePlus
Y255 Phosphorylation PhosphoSitePlus
K259 Acetylation PhosphoSitePlus
K259 Sumoylation PhosphoSitePlus
K259 Ubiquitination PhosphoSitePlus
K260 Acetylation PhosphoSitePlus
K260 Methylation PhosphoSitePlus   UniProt 18183946
K260 Sumoylation PhosphoSitePlus
K260 Ubiquitination PhosphoSitePlus
K263 Acetylation PhosphoSitePlus
K263 Methylation PhosphoSitePlus   UniProt 18183946
K263 Sumoylation PhosphoSitePlus
K263 Ubiquitination PhosphoSitePlus
S266 Phosphorylation PhosphoSitePlus
K271 Ubiquitination PhosphoSitePlus
Y276 Phosphorylation PhosphoSitePlus
S284 Phosphorylation PhosphoSitePlus
S312 Phosphorylation PhosphoSitePlus   UniProt 18183946, 19690332
Y314 Phosphorylation PhosphoSitePlus
Y320 Phosphorylation PhosphoSitePlus
S321 Phosphorylation PhosphoSitePlus
R323 Methylation PhosphoSitePlus
S333 Phosphorylation PhosphoSitePlus   UniProt 23186163
K334 Acetylation PhosphoSitePlus
K334 Methylation PhosphoSitePlus   UniProt 18183946
K334 Ubiquitination PhosphoSitePlus
Site PTM Type PTM Enzyme Score Source PMID
S25 Phosphorylation HPRD 20058876
Y42 Phosphorylation HPRD 15592455
T59 Phosphorylation HPRD 20068231
T75 Phosphorylation HPRD 20068231
S83 Phosphorylation HPRD 19651622, 18669648, 17081983, ...20068231
S151 Phosphorylation HPRD 19413330, 18669648, 20068231
T154 Phosphorylation HPRD 19413330
T176 Phosphorylation HPRD 20068231
T182 Phosphorylation HPRD 20166139, 18669648
T184 Phosphorylation HPRD 20230923, 19562805, 18077418, ...18669648, 20166139, 20068231
S210 Phosphorylation HPRD 20068231
T211 Phosphorylation HPRD 19413330, 18669648, 20068231
T246 Phosphorylation HPRD 18452278


PTM-dependent PPI

PTM type Substrate Site Interactant Association type Source PMID
Phosphorylation P40429 (RPL13A) S77 P04406 (GAPDH) unknown eFIP 29152905


PTM sites affected in variants

Site Variant Source PMID Disease [Sample source]
S51 F51 Biomuta DOID:4159 / skin cancer [ cosmic ]
T52 N52 Biomuta DOID:1324 / lung cancer [ cosmic ]
K55 E55 Biomuta DOID:11054 / urinary bladder cancer [ cosmic, icgc ]
R80 P80 Biomuta DOID:1324 / lung cancer [ cosmic ]
R80 * Biomuta DOID:363 / uterine cancer [ cosmic ]
S83 Y83 Biomuta DOID:4159 / skin cancer [ cosmic ]
T99 S99 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
K107 R107 Biomuta DOID:363 / uterine cancer [ cosmic, icgc ]
K117 R117 Biomuta DOID:10534 / stomach cancer [ cosmic ]
Y140 * Biomuta DOID:363 / uterine cancer [ cosmic ]
T184 A184 Biomuta DOID:1612 / breast cancer [ cosmic, icgc ]
R200 H200 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
R200 L200 Biomuta DOID:4362 / cervical cancer [ cosmic, icgc ]
S210 F210 Biomuta DOID:8618 / oral cavity cancer [ cosmic ]
T211 A211 Biomuta DOID:10534 / stomach cancer [ cosmic, icgc ]
R234 H234 Biomuta DOID:10534 / stomach cancer [ cosmic ]
N239 K239 Biomuta DOID:1319 / brain cancer [ cosmic ]
S266 L266 Biomuta DOID:1324 / lung cancer [ cosmic, icgc ]
S284 F284 Biomuta DOID:1612 / breast cancer [ cosmic, icgc ]
S284 C284 Biomuta DOID:8618 / oral cavity cancer [ cosmic ] DOID:10534 / stomach cancer [ cosmic ] DOID:11934 / head and neck cancer [ icgc ]
S321 R321 Biomuta DOID:1781 / thyroid gland cancer [ cosmic ] DOID:1781 / thyroid cancer [ icgc ]
R323 K323 Biomuta DOID:1324 / lung cancer [ cosmic ]
S333 Y333 Biomuta DOID:4159 / skin cancer [ cosmic, icgc ]