iPTMnet Report for P04049 (RAF1)


Protein Information

Cytoscape View


UniProt AC / UniProt ID P04049 / RAF1_HUMAN
Protein Name RAF proto-oncogene serine/threonine-protein kinase
Gene Name Name: RAF1
Synonyms:RAF;
Organism Homo sapiens (Human)

PRO ID PR:P04049
DAG View
PRO Name RAF proto-oncogene serine/threonine-protein kinase (human)
Definition A RAF proto-oncogene serine/threonine-protein kinase that is encoded in the genome of human.
Short Label hRAF1
Category organism-gene

Interactive Sequence View

Select/align proteoforms across species


P04049 (RAF1) as Substrate



Site PTM Type PTM Enzyme Score Source PMID
S12 Phosphorylation PhosphoSitePlus
K18 Methylation PhosphoSitePlus
S29 Phosphorylation P28482 (MAPK1) neXtProt   phospho.ELM   PRO   PhosphoSitePlus 15664191
T31 Phosphorylation PhosphoSitePlus
Y38 Phosphorylation PhosphoSitePlus
S43 Phosphorylation P17252 (PRKCA) , P17612 (PRKACA) neXtProt   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P   Signor   UniProt 7694366, 11971957, 24853429, ...11027949, 15280158, 8607981, 7935389, 8349614, 12801936, 15899852, 9001246, 9272646, 21734462, 10862777
T49 Phosphorylation PhosphoSitePlus
K53 Ubiquitination PhosphoSitePlus
K65 Ubiquitination PhosphoSitePlus
R100 Methylation PhosphoSitePlus
S211 Phosphorylation PhosphoSitePlus
T213 Phosphorylation PhosphoSitePlus
S220 Phosphorylation PhosphoSitePlus
R223 Methylation PhosphoSitePlus
S227 O-Glycosylation GlyGen 31521821, 33909326
R231 Methylation PhosphoSitePlus
Y232 Phosphorylation PhosphoSitePlus
S233 Phosphorylation P17612 (PRKACA) , P17252 (PRKCA) IEDB   neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor 12801936, 12551925, 22922483, ...11997508, 32387766
T234 Phosphorylation PhosphoSitePlus
T239 Phosphorylation PhosphoSitePlus
T242 Phosphorylation PhosphoSitePlus
S244 Phosphorylation PhosphoSitePlus
S247 Phosphorylation PhosphoSitePlus
S250 Phosphorylation PhosphoSitePlus
S252 Phosphorylation phospho.ELM   PhosphoSitePlus   UniProt 17525332
S257 Phosphorylation phospho.ELM   PhosphoSitePlus   RLIMS-P 18220336, 26659599
T258 Phosphorylation phospho.ELM   PhosphoSitePlus   RLIMS-P 18669648, 26659599, 18220336
S259 Phosphorylation P17612 (PRKACA) , P31749 (AKT1) , P04049 (RAF1) , Q13131 (PRKAA1) , O95835 (LATS1) IntAct   neXtProt   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P   Signor   UniProt 17031853, 16630891, 16239230, ...31285551, 11782426, 12087097, 12691603, 29515772, 16854453, 23529931, 26442853, 16760675, 20149136, 11756411, 15899852, 11341776, 11041243, 20212043, 12083801, 14992682, 12181740, 8321321, 18669648, 10801873, 20934465, 24929361, 8349614, 15772344, 29097117, 23554907, 24853429, 20868520, 29917268, 21734462, 16892053, 18220336, 24922062, 12225966, 17064357, 27527217, 11971957, 20688159, 22487193, 20161842, 14688280, 15047712, 15534860, 14755538, 27534532, 10576742, 10576741, 35044719, 15280158, 18055485, 22182854, 12801936, 18374541, 24732797, 30368668, 24625973, 17785857, 20052757, 23487407, 30909892, 29545175, 26659599
T260 Phosphorylation phospho.ELM   PhosphoSitePlus 18220336
S267 Phosphorylation PhosphoSitePlus
T268 Phosphorylation P04049 (RAF1) PhosphoSitePlus   RLIMS-P   UniProt 8349614, 15642359
T269 Phosphorylation Q8IVT5 (KSR1) neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 7477354, 11134016, 15642359, ...19766101, 9094715
S283 Phosphorylation PhosphoSitePlus
S285 Phosphorylation PhosphoSitePlus
S287 Phosphorylation PhosphoSitePlus
S289 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3) neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P   UniProt 16407412, 21917714, 23554907, ...15664191, 20149136, 19690332
S291 Phosphorylation PhosphoSitePlus
S294 Phosphorylation phospho.ELM   PhosphoSitePlus 17081983
S295 Phosphorylation PhosphoSitePlus
S296 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1) neXtProt   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P   UniProt 16407412, 23186163, 15664191, ...15642359, 20149136, 31541179
S301 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3) neXtProt   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P   Signor   UniProt 16407412, 23186163, 17081983, ...21917714, 15664191, 15642359, 20149136, 19690332, 31541179
T303 Phosphorylation PhosphoSitePlus
S306 Phosphorylation PhosphoSitePlus
T310 Phosphorylation PhosphoSitePlus
T324 Phosphorylation PhosphoSitePlus
S338 Phosphorylation Q02750 (MAP2K1) , P04049 (RAF1) , P53350 (PLK1) , Q13153 (PAK1) , O75914 (PAK3) , Q16512 (PKN1) neXtProt   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P+   RLIMS-P   Signor   UniProt 15849194, 24568222, 31285551, ...9823899, 21917714, 12446733, 18775988, 18821707, 20685651, 26058013, 22592532, 19710016, 28947956, 23653349, 15705600, 26154409, 23987506, 19288477, 18465753, 22096607, 31638257, 31883527, 11274179, 16405965, 19717011, 26333361, 15470067, 17055484, 24023871, 28694330, 15601627, 24853429, 12551923, 10205168, 11376654, 17277048, 11782426, 16892053, 17785857, 26242183, 30586319, 11733498, 16129686, 20015475, 10712905, 26565813, 27003818, 10801448, 17064357, 20141835, 27527217, 19133285, 26442853, 26100670, 15899852, 22158035, 17428866, 31213532, 27534532, 11971957, 12719581, 18064632, 15280158, 11948406, 30971268, 27999210, 29662630, 20509944, 25204017, 9794231, 11259591, 22880011, 20868520, 21596018, 31091164, 31541179
S339 Phosphorylation P53350 (PLK1) , O75914 (PAK3) , Q16512 (PKN1) , Q13153 (PAK1) neXtProt   phospho.ELM   PhosphoSitePlus   RLIMS-P+   RLIMS-P   Signor   UniProt 9794231, 15849194, 9823899, ...27003818, 17428866, 18775988
Y340 Phosphorylation P12931 (SRC) neXtProt   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P   Signor   UniProt 23905676, 9794231, 17492661, ...18064632, 11134016, 12551923, 16892053, 26565813, 9020159, 17064357, 7542586, 10998357, 7692235, 14688280
Y341 Phosphorylation P12931 (SRC) neXtProt   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P   Signor   UniProt 23905676, 16892053, 11376654, ...7542586, 9794231, 12446733, 12551923, 17492661, 26565813, 9020159, 17064357, 22592532, 31213532, 10998357, 11259591, 14688280, 10205168, 28694330
T353 Phosphorylation PhosphoSitePlus
S357 Phosphorylation PhosphoSitePlus
S359 Phosphorylation PhosphoSitePlus
T362 Phosphorylation PhosphoSitePlus
K365 Ubiquitination PhosphoSitePlus
K367 Ubiquitination PhosphoSitePlus
K378 Ubiquitination PhosphoSitePlus
Y458 Phosphorylation PhosphoSitePlus
K462 Ubiquitination PhosphoSitePlus
K470 Ubiquitination PhosphoSitePlus
S471 Phosphorylation phospho.ELM   PhosphoSitePlus   UniProt 16093354
T481 Phosphorylation PhosphoSitePlus
T491 Phosphorylation phospho.ELM   PRO   PhosphoSitePlus   UniProt 15258589, 11447113
K493 Ubiquitination PhosphoSitePlus
S494 Phosphorylation phospho.ELM   PRO   PhosphoSitePlus   UniProt 15258589, 11447113
S497 Phosphorylation P05771 (PRKCB) , P05129 (PRKCG) , P17252 (PRKCA) neXtProt   PhosphoSitePlus   RLIMS-P   Signor 9794231, 12551925, 8288587
S499 Phosphorylation P17252 (PRKCA) phospho.ELM   PhosphoSitePlus   RLIMS-P   Signor   UniProt 9794231, 12551925, 8349614, ...8321321
T543 Phosphorylation PhosphoSitePlus
K575 Ubiquitination PhosphoSitePlus
S612 Phosphorylation PhosphoSitePlus
S619 Phosphorylation P05129 (PRKCG) , P17252 (PRKCA) , P05771 (PRKCB) neXtProt   PhosphoSitePlus   Signor 12551925, 8288587
S621 Phosphorylation P17612 (PRKACA) , P04049 (RAF1) , Q13131 (PRKAA1) IntAct   neXtProt   phospho.ELM   PRO   PhosphoSitePlus   RLIMS-P   Signor   UniProt 16892053, 27003818, 11041243, ...24330068, 21917714, 23554907, 29515772, 10801873, 19298812, 8816453, 22182854, 8349614, 9091312, 24922062, 26295714, 11971957, 19595761, 27331409, 14688280
S624 Phosphorylation PhosphoSitePlus
T638 Phosphorylation PhosphoSitePlus
T640 Phosphorylation PhosphoSitePlus
S642 Phosphorylation P28482 (MAPK1) neXtProt   phospho.ELM   PRO   PhosphoSitePlus   UniProt 18669648, 23186163, 15664191
Site PTM Type PTM Enzyme Score Source PMID
S29 Phosphorylation P28482 (MAPK1) HPRD 18691976, 15664191
S43 Phosphorylation P17612 (PRKACA) HPRD 11971957, 11997508, 18691976
S233 Phosphorylation P17612 (PRKACA) HPRD 11997508
S244 Phosphorylation HPRD 18691976, 20068231
S252 Phosphorylation HPRD 17525332
S257 Phosphorylation HPRD 20166139, 18691976, 20068231
T258 Phosphorylation HPRD 18669648
S259 Phosphorylation P31749 (AKT1) , P17612 (PRKACA) HPRD   PRO 18691976, 11971957, 20230923, ...18767875, 18669648, 11997508, 10576742, 14688280, 20068231
T260 Phosphorylation HPRD 20230923
T268 Phosphorylation P04049 (RAF1) HPRD 8349614
T269 Phosphorylation HPRD 7477354
S289 Phosphorylation P28482 (MAPK1) HPRD 18691976, 15664191
S291 Phosphorylation HPRD 18691976
S294 Phosphorylation HPRD 17081983
S295 Phosphorylation HPRD 18691976
S296 Phosphorylation P28482 (MAPK1) HPRD 19651622, 15664191, 20068231
S301 Phosphorylation P28482 (MAPK1) HPRD 18691976, 15664191, 20068231
T303 Phosphorylation HPRD 19651622, 18767875
S338 Phosphorylation Q15382 (RHEB) , Q13153 (PAK1) , O75914 (PAK3) , P12931 (SRC) HPRD   PRO 15849194, 9823899, 17097642, ...11733498, 12551923, 9234708, 16803888, 10205168
S339 Phosphorylation P12931 (SRC) HPRD   PRO 17097642, 15849194, 9234708
Y340 Phosphorylation P12931 (SRC) , O60674 (JAK2) HPRD 17097642, 9689060, 8876196, ...11134016
Y341 Phosphorylation P12931 (SRC) , O60674 (JAK2) HPRD 17097642, 8876196, 10205168
T491 Phosphorylation HPRD 11447113
S494 Phosphorylation HPRD 11447113
S499 Phosphorylation P17252 (PRKCA) HPRD 8321321
S619 Phosphorylation HPRD 20068231
S621 Phosphorylation P04049 (RAF1) , P17612 (PRKACA) , Q13131 (PRKAA1) HPRD   PRO 9710607, 11971957, 19007248, ...9091312, 19651622, 20166139, 14688280, 20068231
S624 Phosphorylation HPRD 20068231
T641 Phosphorylation HPRD 18691976
S642 Phosphorylation P28482 (MAPK1) HPRD 18767875, 18669648, 15664191


P04049 (RAF1) as PTM Enzyme



Substrate Site Score Source PMID
O14974 (PPP1R12A) T696 neXtProt   PhosphoSitePlus 11719507
O43306 (ADCY6) S732 neXtProt 11327830
O43306 (ADCY6) S734 neXtProt 11327830
O43306 (ADCY6) S738 neXtProt 11327830
O43306 (ADCY6) S742 neXtProt 11327830
P04049 (RAF1) S259 PhosphoSitePlus 8321321
P04049 (RAF1) T268 HPRD   UniProt 8349614
P04049 (RAF1) S338 PhosphoSitePlus 18775988
P04049 (RAF1) S621 HPRD   PhosphoSitePlus 9710607, 11971957, 8816453, ...19007248, 9091312, 19651622, 20166139, 19595761, 20068231
P28482-1 (MAPK1) S29 HPRD 15664191
P36507 (MAP2K2) S222 Signor 8157000
P36507 (MAP2K2) S226 Signor 8157000
P45379 (TNNT2) T213 neXtProt 19381846
Q02750 (MAP2K1) S218 HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   Signor 10359597, 16585161, 7731720, ...8157000, 8131746, 11042690, 8621729, 10409742, 8394352
Q02750-1 (MAP2K1) S222 HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   Signor 10359597, 8131746, 7731720, ...16585161, 8157000, 11042690, 8621729, 8394352
Q02750-1 (MAP2K1) T286 HPRD 11684694, 18691976
Q02750-1 (MAP2K1) T292 HPRD 8157000
Q02750-1 (MAP2K1) T386 HPRD 8157000, 18691976
Q05639 (EEF1A2) S21 PhosphoSitePlus 22378069
Q13118 (KLF10) T93 PhosphoSitePlus 23994618
Q92934 (BAD) S75 neXtProt   PhosphoSitePlus   Signor 15849194, 19667065
Q92934 (BAD) S99 neXtProt   PhosphoSitePlus 19667065
Q92934 (BAD) S118 neXtProt   PhosphoSitePlus   Signor 19667065, 17535812


Proteoforms

PRO ID (Short Label) Sites PTM Enzyme Source PMID
PR:000048997 (hRAF1/Phos:3) pS259,pS621 PRO
PR:000048998 (hRAF1/Phos:4) pS29,pS43,pS259,pS296,pS301,pS338,pY341,pT491,pS494,pS621,pS642 PRO
PR:000048999 (hRAF1/Phos:5) pS338 PRO
PR:000049000 (hRAF1/Phos:6) pS338,pY340,pY341 PRO
PR:000049001 (hRAF1/Phos:7) pS338,pY341,pT491,pS494,pS621 PRO
PR:000049002 (hRAF1/Phos:8) pS621 PRO
PR:000049003 (hRAF1/Phos:9) pY341,pT491,pS494,pS621 PRO
PR:000026210 (hRAF1/iso:1/Phos:3) pS259,pS621 PRO 14688280
PR:000036643 (hRAF1/iso:1/Phos:2) pS338,pS339 PRO 15849194


PTM-dependent PPI

PTM type Substrate Site Interactant Association type Source PMID
Phosphorylation P04049 (RAF1) S259 P07948 (LYN) increased_association eFIP 22182854
Phosphorylation P04049 (RAF1) S338 O96017 (CHEK2) unknown eFIP 26333361
Phosphorylation P04049 (RAF1) S43 P01112 (HRAS) decreased_association eFIP 9001246
Phosphorylation P04049 (RAF1) S259 Q13188 (STK3) increased_association eFIP 27527217
Phosphorylation P04049 (RAF1) S43 Q15382 (RHEB) decreased_association eFIP 9001246
Phosphorylation P30086 (PEBP1) S153 P04049 (RAF1) unknown eFIP 19551145
Phosphorylation P30086 (PEBP1) T42 P04049 (RAF1) unknown eFIP 25104559
Phosphorylation P30086 (PEBP1) S153 P04049 (RAF1) unknown eFIP 21689459
Phosphorylation P35232 (PHB1) T258 P04049 (RAF1) unknown eFIP 29784973
Phosphorylation Q13188 (STK3) Y81 P04049 (RAF1) unknown eFIP 22590567
Phosphorylation Q96TC7 (RMDN3) S46 P04049 (RAF1) unknown eFIP 30360441
Phosphorylation Q96TC7 (RMDN3) Y176 P04049 (RAF1) unknown eFIP 28754031


Proteoform PPIs

Protein 1 Relation Protein 2 Source PMID
PR:000036643 (hRAF1/iso:1/Phos:2) Interaction PR:P10415 (hBCL2) PRO 15849194
PR:000044747 (mBAD/iso:1/UnMod) Interaction PR:P04049 (hRAF1) PRO 17011751
PR:O60566 (hBUB1B) Interaction PR:P04049 (hRAF1) PRO 20703093
PR:P04049 (hRAF1) Interaction PR:O60566 (hBUB1B) PRO 20703093


PTM sites affected in variants

Site Variant Source PMID Disease [Sample source]
S12 C12 Biomuta DOID:3571 / liver cancer [ cosmic ]
T31 P31 Biomuta DOID:1612 / breast cancer [ cosmic, icgc ]
S220 F220 Biomuta DOID:4159 / skin cancer [ cosmic ]
R223 M223 Biomuta DOID:10534 / stomach cancer [ cosmic, icgc ]
R231 I231 Biomuta DOID:363 / uterine cancer [ icgc ]
S244 R244 Biomuta DOID:3571 / liver cancer [ cosmic ]
S257 W257 Biomuta DOID:1324 / lung cancer [ cosmic, icgc ]
S257 L257 Biomuta [ cosmic ] DOID:4606 / bile duct cancer [ cosmic ] DOID:1324 / lung cancer [ cosmic, icgc ] DOID:4159 / skin cancer [ cosmic, icgc ] DOID:1245 / vulva cancer [ cosmic ] DOID:1793 / pancreatic cancer [ cosmic ] DOID:10534 / stomach cancer [ cosmic, icgc ] DOID:2394 / ovarian cancer [ cosmic ] DOID:9253 / gastrointestinal stromal tumor [ cosmic ] DOID:9256 / colorectal cancer [ cosmic, icgc ] DOID:11054 / urinary bladder cancer [ cosmic ]
S259 P259 Biomuta [ cosmic ] DOID:4606 / bile duct cancer [ cosmic ] DOID:1324 / lung cancer [ cosmic ] DOID:2531 / hematologic cancer [ icgc ] DOID:9256 / colorectal cancer [ cosmic ] DOID:10534 / stomach cancer [ cosmic ]
S259 F259 Biomuta [ cosmic ] DOID:1324 / lung cancer [ cosmic, icgc ] DOID:4159 / skin cancer [ cosmic, icgc ] DOID:1781 / thyroid gland cancer [ cosmic ] DOID:8618 / oral cavity cancer [ cosmic ] DOID:9253 / gastrointestinal stromal tumor [ cosmic ] DOID:11054 / urinary bladder cancer [ cosmic ]
S259 A259 Biomuta DOID:2394 / ovarian cancer [ cosmic ]
S259 C259 Biomuta DOID:4159 / skin cancer [ cosmic ] DOID:1324 / lung cancer [ cosmic ]
T260 I260 Biomuta DOID:1324 / lung cancer [ cosmic ]
T260 K260 Biomuta DOID:1324 / lung cancer [ cosmic ]
S287 * Biomuta DOID:4159 / skin cancer [ cosmic ]
S289 L289 Biomuta DOID:1324 / lung cancer [ cosmic ]
S291 L291 Biomuta DOID:4159 / skin cancer [ cosmic ] DOID:10534 / stomach cancer [ cosmic ]
S295 G295 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
S295 C295 Biomuta DOID:1324 / lung cancer [ cosmic ]
S301 R301 Biomuta DOID:3571 / liver cancer [ cosmic ]
T303 A303 Biomuta DOID:11054 / urinary bladder cancer [ cosmic, icgc ]
S339 I339 Biomuta DOID:363 / uterine cancer [ icgc ]
T353 I353 Biomuta DOID:2531 / hematologic cancer [ cosmic, icgc ]
S359 C359 Biomuta DOID:1324 / lung cancer [ cosmic ]
K365 R365 Biomuta DOID:363 / uterine cancer [ cosmic ]
Y458 F458 Biomuta DOID:1793 / pancreatic cancer [ cosmic ]
T491 I491 Biomuta DOID:4159 / skin cancer [ cosmic ]
K493 T493 Biomuta DOID:4159 / skin cancer [ cosmic ]
S612 Y612 Biomuta DOID:363 / uterine cancer [ icgc ]