iPTMnet Report for O75116 (ROCK2)


Protein Information

Cytoscape View


UniProt AC / UniProt ID O75116 / ROCK2_HUMAN
Protein Name Rho-associated protein kinase 2
Gene Name Name: ROCK2
Synonyms:KIAA0619;
Organism Homo sapiens (Human)

PRO ID PR:O75116
DAG View
PRO Name Rho-associated protein kinase 2 (human)
Definition A Rho-associated protein kinase 2 that is encoded in the genome of human.
Short Label hROCK2
Category organism-gene

Interactive Sequence View

Select/align proteoforms across species


O75116 (ROCK2) as Substrate



Site PTM Type PTM Enzyme Score Source PMID
T16 Phosphorylation PhosphoSitePlus
S25 Phosphorylation PhosphoSitePlus
K29 Ubiquitination PhosphoSitePlus
Y92 Phosphorylation PhosphoSitePlus
K96 Ubiquitination PhosphoSitePlus
K121 Ubiquitination PhosphoSitePlus
K125 Ubiquitination PhosphoSitePlus
S134 Phosphorylation PhosphoSitePlus
K216 Ubiquitination PhosphoSitePlus
C236 Methylation IEDB 31844290
T249 Phosphorylation PhosphoSitePlus
Y256 Phosphorylation RLIMS-P 28956212
S298 Phosphorylation PhosphoSitePlus
K323 Ubiquitination PhosphoSitePlus
K355 Ubiquitination PhosphoSitePlus
K391 Ubiquitination PhosphoSitePlus
T396 Phosphorylation PhosphoSitePlus
T414 Phosphorylation O75116 (ROCK2) neXtProt 19099536
S423 O-Glycosylation GlyGen 23301498, 30379171, 35254053
S423 Phosphorylation PhosphoSitePlus
S425 O-Glycosylation GlyGen 23301498, 30379171, 35254053
S425 Phosphorylation HPRD   PhosphoSitePlus 17322306, 18691976, 18669648
S427 O-Glycosylation GlyGen 23301498, 30379171, 35254053
S433 O-Glycosylation GlyGen 23301498, 30379171, 35254053
S436 O-Glycosylation GlyGen 23301498, 30379171, 35254053
K448 Ubiquitination PhosphoSitePlus
K482 Ubiquitination PhosphoSitePlus
K492 Ubiquitination PhosphoSitePlus
K505 Ubiquitination PhosphoSitePlus
K511 Ubiquitination PhosphoSitePlus
K537 Ubiquitination PhosphoSitePlus
S550 Phosphorylation PhosphoSitePlus
S553 Phosphorylation PhosphoSitePlus
K556 Ubiquitination PhosphoSitePlus
T567 Phosphorylation HPRD   PhosphoSitePlus 20068231
T573 Phosphorylation PhosphoSitePlus
S575 Phosphorylation PhosphoSitePlus
T577 Phosphorylation PhosphoSitePlus
K590 Ubiquitination PhosphoSitePlus
K605 Ubiquitination PhosphoSitePlus
K618 Ubiquitination PhosphoSitePlus
K669 Ubiquitination PhosphoSitePlus
K709 Ubiquitination PhosphoSitePlus
K720 Ubiquitination PhosphoSitePlus
Y722 Phosphorylation P12931 (SRC) HPRD   neXtProt   PhosphoSitePlus   UniProt 20826462, 18559669, 18707149
S724 Phosphorylation PhosphoSitePlus
K729 Ubiquitination PhosphoSitePlus
K749 Ubiquitination PhosphoSitePlus
K759 Ubiquitination PhosphoSitePlus
K795 Ubiquitination PhosphoSitePlus
K860 Ubiquitination PhosphoSitePlus
K877 Ubiquitination PhosphoSitePlus
S930 Phosphorylation PhosphoSitePlus
Y936 Phosphorylation PhosphoSitePlus
S937 Phosphorylation PhosphoSitePlus
K946 Acetylation PhosphoSitePlus
K951 Ubiquitination PhosphoSitePlus
K964 Ubiquitination PhosphoSitePlus
T967 Phosphorylation P53350 (PLK1) neXtProt 17446864
K989 Ubiquitination PhosphoSitePlus
K995 Ubiquitination PhosphoSitePlus
K997 Ubiquitination PhosphoSitePlus
K1007 Ubiquitination PhosphoSitePlus
K1017 Ubiquitination PhosphoSitePlus
K1022 Ubiquitination PhosphoSitePlus
K1071 Ubiquitination PhosphoSitePlus
T1078 Phosphorylation HPRD   phospho.ELM   PhosphoSitePlus 17525332
K1083 Ubiquitination PhosphoSitePlus
K1086 Ubiquitination PhosphoSitePlus
S1099 Phosphorylation P53350 (PLK1) neXtProt 17446864
S1114 Phosphorylation PhosphoSitePlus
S1121 Phosphorylation HPRD 18691976
S1132 Phosphorylation HPRD   phospho.ELM   PhosphoSitePlus 18669648, 20068231
S1133 Phosphorylation P53350 (PLK1) HPRD   neXtProt   phospho.ELM   PhosphoSitePlus 18691976, 18669648, 17446864, ...20068231
S1134 Phosphorylation HPRD   phospho.ELM   PhosphoSitePlus 18669648, 15302935, 20068231
S1137 Phosphorylation HPRD   phospho.ELM   PhosphoSitePlus   UniProt 18691976, 21406692, 15302935, ...18669648, 19690332, 20068231
T1165 Phosphorylation PhosphoSitePlus
K1181 Ubiquitination PhosphoSitePlus
S1187 Phosphorylation PhosphoSitePlus
S1194 Phosphorylation PhosphoSitePlus
Y1197 Phosphorylation PhosphoSitePlus
T1212 Phosphorylation PhosphoSitePlus   UniProt 23186163
K1222 Ubiquitination PhosphoSitePlus
Y1232 Phosphorylation PhosphoSitePlus
S1238 Phosphorylation PhosphoSitePlus
K1252 Ubiquitination PhosphoSitePlus
Y1255 Phosphorylation PhosphoSitePlus
C1315 Methylation IEDB 31844290
K1316 Ubiquitination PhosphoSitePlus
Y1318 Phosphorylation PhosphoSitePlus
Y1319 Phosphorylation PhosphoSitePlus
T1334 Phosphorylation PhosphoSitePlus
K1339 Ubiquitination PhosphoSitePlus
K1350 Acetylation PhosphoSitePlus
K1351 Acetylation PhosphoSitePlus
K1351 Ubiquitination PhosphoSitePlus
S1361 Phosphorylation HPRD   PhosphoSitePlus 18691976, 17081983
S1362 Phosphorylation HPRD   PhosphoSitePlus   UniProt 23186163, 17081983
T1365 Phosphorylation PhosphoSitePlus
S1366 Phosphorylation PhosphoSitePlus   RLIMS-P 32647287
K1368 Methylation PhosphoSitePlus
K1368 Ubiquitination PhosphoSitePlus
S1374 Phosphorylation P53350 (PLK1) HPRD   neXtProt   phospho.ELM   PhosphoSitePlus   UniProt 17446864, 23186163, 18212344, ...16964243
S1379 Phosphorylation IEDB   PhosphoSitePlus   RLIMS-P 35494241, 24610576
S1388 Phosphorylation PhosphoSitePlus
Site PTM Type PTM Enzyme Score Source PMID
T1078 Phosphorylation PRO 17525332
S1134 Phosphorylation PRO 17446864, 15302935
S1137 Phosphorylation PRO 17446864, 15302935


O75116 (ROCK2) as PTM Enzyme



Substrate Site Score Source PMID
O14974 (PPP1R12A) T443 neXtProt 10579722
O14974 (PPP1R12A) T696 neXtProt   PhosphoSitePlus 20801872, 10579722
O14974 (PPP1R12A) S852 neXtProt 11331307, 10579722
O14974-1 (PPP1R12A) T853 HPRD 10601309, 12220642, 11931630, ...12563012, 12600888
O75116 (ROCK2) T414 neXtProt 19099536
P05067-4 (APP) T654 PhosphoSitePlus 24305806
P08670 (VIM) S39 neXtProt 9565595
P08670 (VIM) S72 neXtProt 9565595
P15311 (EZR) T567 neXtProt 9456324
P19634 (SLC9A1) T653 PhosphoSitePlus 25578862
P24844 (MYL9) T19 Signor 8702756
P24844 (MYL9) S20 neXtProt   Signor 17015463, 8702756
P26038 (MSN) T558 neXtProt 9456324
P29474 (NOS3) T495 neXtProt 17651694
P35241 (RDX) T564 neXtProt 9456324
P35568 (IRS1) S636 neXtProt 16098829
P35568 (IRS1) S974 neXtProt 16098829
P35611 (ADD1) T445 neXtProt 10209029
P35611 (ADD1) T480 neXtProt 10209029
P51911 (CNN1) T170 neXtProt 10873572
P51911 (CNN1) S175 neXtProt 10873572
P51911 (CNN1) T180 neXtProt 10873572
P51911 (CNN1) T184 neXtProt 10873572
P51911 (CNN1) T259 neXtProt 10873572
P53671 (LIMK2) T505 neXtProt   PhosphoSitePlus 19099536
P56817 (BACE1) S498 PhosphoSitePlus 24305806
P68104 (EEF1A1) T432 PhosphoSitePlus 24405853
Q15306 (IRF4) S447 Signor 20697158
Q15306 (IRF4) S448 Signor 20697158
Q15417 (CNN3) S293 PhosphoSitePlus 20861310
Q16555 (DPYSL2) T555 neXtProt   phospho.ELM   PhosphoSitePlus   Signor 10818093
Q92673 (SORL1) S2206 PhosphoSitePlus 21147781
Q96A00 (PPP1R14A) T38 neXtProt   PhosphoSitePlus   Signor 32471307, 10869555, 12144526
Q96C90 (PPP1R14B) T57 PhosphoSitePlus 12144526
Q99962 (SH3GL2) T14 PhosphoSitePlus   Signor 16164598


Proteoforms

PRO ID (Short Label) Sites PTM Enzyme Source PMID
PR:000036478 (hROCK2/iso:1/Phos:1) pS1134,pS1137 PRO 15302935, 17446864
PR:000036479 (hROCK2/iso:1/Phos:2) pT1078 PRO 17525332


PTM-dependent PPI

PTM type Substrate Site Interactant Association type Source PMID
Phosphorylation P06748 (NPM1) T199 O75116 (ROCK2) increased_association eFIP 26536659
Phosphorylation P10916 (MYL2) S15 O75116 (ROCK2) unknown eFIP 25412762


PTM sites affected in variants

Site Variant Source PMID Disease [Sample source]
S25 N25 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
K121 T121 Biomuta DOID:4159 / skin cancer [ cosmic, icgc ]
K125 T125 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
T577 S577 Biomuta DOID:1319 / brain cancer [ cosmic, icgc ]
K709 E709 Biomuta DOID:363 / uterine cancer [ cosmic, icgc ]
Y722 N722 Biomuta DOID:8618 / oral cavity cancer [ cosmic ]
K729 R729 Biomuta DOID:4362 / cervical cancer [ cosmic, icgc ]
S937 C937 Biomuta DOID:8618 / oral cavity cancer [ cosmic ] DOID:11934 / head and neck cancer [ icgc ] DOID:11054 / urinary bladder cancer [ cosmic, icgc ]
K1017 E1017 Biomuta DOID:1324 / lung cancer [ cosmic ]
S1187 N1187 Biomuta DOID:1612 / breast cancer [ cosmic, icgc, tcga ]
S1194 P1194 Biomuta DOID:4159 / skin cancer [ cosmic ]
K1350 Q1350 Biomuta DOID:10534 / stomach cancer [ cosmic ]
S1361 L1361 Biomuta DOID:1324 / lung cancer [ cosmic, icgc ]
S1362 C1362 Biomuta DOID:11054 / urinary bladder cancer [ cosmic, icgc ]
S1366 * Biomuta DOID:8618 / oral cavity cancer [ cosmic ]
S1379 N1379 Biomuta DOID:1319 / brain cancer [ cosmic, icgc ]