iPTMnet Report for O60885 (BRD4)


Protein Information

Cytoscape View


UniProt AC / UniProt ID O60885 / BRD4_HUMAN
Protein Name Bromodomain-containing protein 4
Gene Name Name: BRD4
Synonyms:HUNK1;
Organism Homo sapiens (Human)

PRO ID PR:O60885
DAG View
PRO Name bromodomain-containing protein 4 (human)
Definition A bromodomain-containing protein 4 that is encoded in the genome of human.
Short Label hBRD4
Category organism-gene

Interactive Sequence View

Select/align proteoforms across species


O60885 (BRD4) as Substrate



Site PTM Type PTM Enzyme Score Source PMID
S5 Phosphorylation RLIMS-P 23027873
Y65 Phosphorylation PhosphoSitePlus
K91 Sumoylation PhosphoSitePlus
K91 Ubiquitination PhosphoSitePlus
Y97 Phosphorylation PhosphoSitePlus   RLIMS-P 34290255
Y98 Phosphorylation PhosphoSitePlus   RLIMS-P 34290255
K99 Methylation PhosphoSitePlus
K99 Ubiquitination PhosphoSitePlus
T103 Phosphorylation PhosphoSitePlus
T109 Phosphorylation PhosphoSitePlus
K111 Acetylation PhosphoSitePlus
K111 Ubiquitination PhosphoSitePlus
K155 Ubiquitination PhosphoSitePlus
K177 Methylation PhosphoSitePlus
R179 Methylation PhosphoSitePlus
R181 Methylation PhosphoSitePlus
R183 Methylation PhosphoSitePlus
T204 Phosphorylation PhosphoSitePlus   RLIMS-P 28481868
T221 Phosphorylation IEDB 32387766
T249 Phosphorylation RLIMS-P 32575711
T296 Phosphorylation PhosphoSitePlus
T299 Phosphorylation PhosphoSitePlus
T315 Phosphorylation PhosphoSitePlus
T316 Phosphorylation PhosphoSitePlus
K317 Acetylation PhosphoSitePlus
S324 Phosphorylation PhosphoSitePlus
S325 Phosphorylation PhosphoSitePlus
K333 Acetylation PhosphoSitePlus
S338 Phosphorylation PhosphoSitePlus
K346 Acetylation PhosphoSitePlus
S347 O-Glycosylation GlyGen 34846842, 33214551, 30379171, ...32574038, 26853435, 30059200, 25367160, 35254053, 34019948, 30620550, 29351928, 34229054, 20068230
S348 O-Glycosylation GlyGen 34846842, 33214551, 30379171, ...32574038, 26853435, 30059200, 25367160, 35254053, 34019948, 30620550, 29351928, 34229054, 20068230
S351 Phosphorylation PhosphoSitePlus
S358 Phosphorylation PhosphoSitePlus
K362 Acetylation PhosphoSitePlus
K367 Acetylation PhosphoSitePlus
K368 Acetylation PhosphoSitePlus
K404 Methylation PhosphoSitePlus
K404 Ubiquitination PhosphoSitePlus
K406 Methylation PhosphoSitePlus
R453 Methylation PhosphoSitePlus
S469 Phosphorylation phospho.ELM   PhosphoSitePlus 18669648
S470 Phosphorylation phospho.ELM   PhosphoSitePlus   UniProt 18669648, 15302935, 24275569
S484 Phosphorylation PhosphoSitePlus   RLIMS-P   UniProt 23317504, 28733670, 24466310
S488 Phosphorylation PhosphoSitePlus   RLIMS-P   UniProt 23317504, 28733670, 24466310
S492 Phosphorylation PhosphoSitePlus   RLIMS-P   UniProt 23317504, 31292434, 31832310
S494 Phosphorylation PhosphoSitePlus   RLIMS-P   UniProt 23317504, 31292434, 31832310
S498 Phosphorylation PhosphoSitePlus   UniProt 23317504
S499 Phosphorylation PhosphoSitePlus   UniProt 23317504
T500 Phosphorylation PhosphoSitePlus
S503 Phosphorylation PhosphoSitePlus   UniProt 23317504
T573 O-Glycosylation GlyGen 25367160
S578 Phosphorylation PhosphoSitePlus
S579 Phosphorylation PhosphoSitePlus
S581 Phosphorylation PhosphoSitePlus
S584 Phosphorylation PhosphoSitePlus
T598 Phosphorylation PhosphoSitePlus
Y599 Phosphorylation PhosphoSitePlus
S601 Phosphorylation phospho.ELM   PhosphoSitePlus   UniProt 17287340, 21406692, 23186163, ...20068231
S619 Phosphorylation PhosphoSitePlus
K624 Ubiquitination PhosphoSitePlus
K629 Ubiquitination PhosphoSitePlus
T658 O-Glycosylation GlyGen 34846842, 33214551, 30379171, ...32574038, 26853435, 30059200, 25367160, 35254053, 34019948, 30620550, 29351928, 34229054, 20068230
K660 Ubiquitination PhosphoSitePlus
S673 Phosphorylation PhosphoSitePlus
K685 Acetylation PhosphoSitePlus
K694 Acetylation PhosphoSitePlus
K694 Sumoylation PhosphoSitePlus
K694 Ubiquitination PhosphoSitePlus
S705 Phosphorylation PhosphoSitePlus
K726 Acetylation PhosphoSitePlus
K727 Acetylation PhosphoSitePlus
S858 Phosphorylation PhosphoSitePlus
T942 Phosphorylation PhosphoSitePlus
S1045 Phosphorylation PhosphoSitePlus   RLIMS-P 32575711
K1050 Sumoylation PhosphoSitePlus
S1051 Phosphorylation PhosphoSitePlus
Y1054 Phosphorylation PhosphoSitePlus
S1064 Phosphorylation PhosphoSitePlus
S1070 Phosphorylation PhosphoSitePlus
S1074 Phosphorylation PhosphoSitePlus
T1080 Phosphorylation PhosphoSitePlus
S1083 Phosphorylation PhosphoSitePlus
S1100 Phosphorylation PhosphoSitePlus
K1111 Acetylation PhosphoSitePlus   UniProt 19608861
K1111 Sumoylation PhosphoSitePlus
K1114 Acetylation PhosphoSitePlus
S1117 Phosphorylation phospho.ELM   PhosphoSitePlus   RLIMS-P   UniProt 21406692, 17081983, 18220336, ...32575711, 18669648, 24275569, 20068231
S1126 Phosphorylation PhosphoSitePlus   RLIMS-P   UniProt 32575711, 23186163
T1186 Phosphorylation PhosphoSitePlus
K1197 Sumoylation PhosphoSitePlus
S1201 Phosphorylation PhosphoSitePlus   UniProt 23186163
S1204 Phosphorylation PhosphoSitePlus   UniProt 23186163
T1211 O-Glycosylation GlyGen 34846842, 33214551, 30379171, ...32574038, 26853435, 30059200, 25367160, 35254053, 34019948, 30620550, 29351928, 34229054, 20068230
T1211 Phosphorylation PhosphoSitePlus
T1212 O-Glycosylation GlyGen 34846842, 33214551, 30379171, ...32574038, 26853435, 30059200, 25367160, 35254053, 34019948, 30620550, 29351928, 34229054, 20068230
T1212 Phosphorylation PhosphoSitePlus
S1214 O-Glycosylation GlyGen 34846842, 33214551, 30379171, ...32574038, 26853435, 30059200, 25367160, 35254053, 34019948, 30620550, 29351928, 34229054, 20068230
S1215 O-Glycosylation GlyGen   PhosphoSitePlus 34846842, 33214551, 30379171, ...32574038, 26853435, 30059200, 25367160, 35254053, 34019948, 30620550, 29351928, 34229054, 20068230
T1216 O-Glycosylation GlyGen 34846842, 33214551, 30379171, ...32574038, 26853435, 30059200, 25367160, 35254053, 34019948, 30620550, 29351928, 34229054, 20068230
K1218 Ubiquitination PhosphoSitePlus
S1219 O-Glycosylation GlyGen 34846842, 33214551, 30379171, ...32574038, 26853435, 30059200, 25367160, 35254053, 34019948, 30620550, 29351928, 34229054, 20068230
S1223 O-Glycosylation GlyGen 34846842, 33214551, 30379171, ...32574038, 26853435, 30059200, 25367160, 35254053, 34019948, 30620550, 29351928, 34229054, 20068230
S1223 Phosphorylation PhosphoSitePlus
Site PTM Type PTM Enzyme Score Source PMID
S325 Phosphorylation HPRD 20068231
S469 Phosphorylation HPRD 19413330, 18669648, 18491316, ...18452278
S470 Phosphorylation HPRD 19413330, 18491316, 15302935, ...19664995, 18452278, 18669648, 20068231
S601 Phosphorylation HPRD 19413330, 19651622, 17287340, ...20068231, 18452278
S795 Phosphorylation HPRD 17081983
T803 Phosphorylation HPRD 20068231
S1045 Phosphorylation HPRD 17081983
S1117 Phosphorylation HPRD 18212344, 18767875, 17322306, ...18669648, 20068231, 17924679
Site PTM Type PTM Enzyme Score Source PMID
S325 Phosphorylation HPRD 20068231
S469 Phosphorylation HPRD 19413330, 18669648, 18491316, ...18452278
S470 Phosphorylation HPRD 19413330, 18491316, 15302935, ...19664995, 18452278, 18669648, 20068231
S601 Phosphorylation HPRD 19413330, 19651622, 17287340, ...20068231, 18452278
S701 Phosphorylation PhosphoSitePlus
S706 Phosphorylation PhosphoSitePlus
S711 Phosphorylation PhosphoSitePlus


O60885 (BRD4) as PTM Enzyme



Substrate Site Score Source PMID
P24928 (POLR2A) S1616 PhosphoSitePlus   Signor 22509028


PTM-dependent PPI

PTM type Substrate Site Interactant Association type Source PMID
Phosphorylation O60885 (BRD4) Y98 Q8NFT8 (DNER) decreased_association eFIP 34290255
Phosphorylation O60885 (BRD4) T204 P50750 (CDK9) unknown eFIP 28481868
Phosphorylation O60885 (BRD4) S484 Q13526 (PIN1) unknown eFIP 28481868
Phosphorylation Q8N6T7 (SIRT6) S338 O60885 (BRD4) inhibited_association eFIP 30518851


PTM sites affected in variants

Site Variant Source PMID Disease [Sample source]
S5 I5 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
K155 Q155 Biomuta DOID:363 / uterine cancer [ icgc ]
K177 T177 Biomuta DOID:10534 / stomach cancer [ cosmic ]
R181 P181 Biomuta DOID:10534 / stomach cancer [ cosmic ]
R181 C181 Biomuta DOID:9256 / colorectal cancer [ cosmic ] DOID:2394 / ovarian cancer [ cosmic ]
R181 H181 Biomuta DOID:4362 / cervical cancer [ cosmic ]
T296 P296 Biomuta DOID:1612 / breast cancer [ cosmic, icgc ] DOID:2531 / hematologic cancer [ cosmic ]
T296 I296 Biomuta DOID:1612 / breast cancer [ cosmic ]
K333 N333 Biomuta DOID:1612 / breast cancer [ cosmic ]
K346 N346 Biomuta DOID:11054 / urinary bladder cancer [ cosmic, icgc ]
R453 C453 Biomuta DOID:363 / uterine cancer [ cosmic, icgc ]
S470 F470 Biomuta [ cosmic ] DOID:4159 / skin cancer [ cosmic, icgc ]
S488 G488 Biomuta DOID:9256 / colorectal cancer [ icgc ]
S492 G492 Biomuta [ cosmic ]
S492 N492 Biomuta DOID:9256 / colorectal cancer [ cosmic ]
S499 L499 Biomuta DOID:363 / uterine cancer [ icgc ] DOID:4159 / skin cancer [ cosmic ] DOID:9256 / colorectal cancer [ cosmic ]
S503 C503 Biomuta DOID:1324 / lung cancer [ cosmic ]
T573 M573 Biomuta DOID:363 / uterine cancer [ icgc ] DOID:9256 / colorectal cancer [ cosmic, icgc ]
S579 R579 Biomuta DOID:1319 / brain cancer [ cosmic, icgc ]
T598 M598 Biomuta DOID:9256 / colorectal cancer [ cosmic ] DOID:10534 / stomach cancer [ cosmic, icgc ]
S619 R619 Biomuta DOID:2531 / hematologic cancer [ cosmic ]
S673 F673 Biomuta DOID:4159 / skin cancer [ cosmic ]
K694 * Biomuta DOID:263 / kidney cancer [ cosmic ]
S706 F706 Biomuta [ cosmic ] DOID:4159 / skin cancer [ cosmic, icgc ]
S711 F711 Biomuta DOID:4159 / skin cancer [ cosmic ]
S858 F858 Biomuta DOID:4159 / skin cancer [ cosmic ]
T942 M942 Biomuta [ cosmic ] DOID:4159 / skin cancer [ cosmic, icgc ] DOID:10534 / stomach cancer [ cosmic, icgc ]
S1045 L1045 Biomuta DOID:4159 / skin cancer [ cosmic ]
S1051 L1051 Biomuta DOID:9256 / colorectal cancer [ cosmic ] DOID:1319 / brain cancer [ icgc ]
S1070 F1070 Biomuta DOID:4159 / skin cancer [ cosmic ]
S1074 * Biomuta DOID:1324 / lung cancer [ cosmic ]
S1100 F1100 Biomuta DOID:4159 / skin cancer [ cosmic ] DOID:1324 / lung cancer [ cosmic, icgc ]
S1214 F1214 Biomuta DOID:4159 / skin cancer [ cosmic ]
S1215 F1215 Biomuta DOID:4159 / skin cancer [ cosmic, icgc ]
K1218 T1218 Biomuta DOID:9256 / colorectal cancer [ cosmic ]